Anti lysozyme antibody

Polyclonal anti-mouse SPRR2A was produced in rabbits by Pacific Immunology (Ramona, CA). The synthesized peptide (CLPSVWPGP) was conjugated to a carrier protein and the conjugate was used to generate the antibody. Other antibodies were purchased from various vendors: anti-human SPRR2A antibody (Abcam, ab125385), anti-MUC2 antibody (Thermo Fisher, MA5-12345), anti-Lysozyme antibody (Santa Cruz, sc-27958), anti-DCLK1 antibody (Abcam, ab202754), anti-GAPDH antibody (Thermo Fisher, PA1-988), anti-β-actin antibody (Cell Signaling, 8457S), anti-β-tubulin (Cell Signaling, 2128S). Fluorescein isothiocyanate (FITC)-conjugated UEA-1 lectin was purchased from GeneTex (GTX01512).

Fetal bovine serum (FBS), Dulbecco’s Modified Eagle’s Medium (DMEM), Minimum Essential Medium Eagle (MEM), palmitic acid (PA), Lipopolysaccharide (LPS), muramyl dipeptide (MDP), n-acetylcysteine (NAC), neurotensin (NT), PKCτ and the non-target control (NTC) MISSION shRNA bacterial glycerol stock and β-actin antibody were from Sigma-Aldrich (St. Louis, MO). Alexa Fluor 594-conjugated NF-κB p65 antibody and anti-Lysozyme antibody for immunohistochemical (IHC) staining were from Santa Cruz (Dallas, TX). anti-Lysozyme antibody for immunofluorescent (IF) staining was from Agilent (Santa Clara, CA). Noggin-conditioned medium was purchased from U-Protein Express BV (Netherlands). Advanced DMEM/F12 medium, growth factor-reduced Matrigel, B-27 Supplement, N-2 Supplement, HEPES, GlutaMAX and Zeocin were from ThermoFisher (Grand Island, NY). Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Alexa Fluor 488 Secondary Antibody and Alexa Fluor 594 Phalloidin were from ThermoFisher. Mouse epidermal growth factor (EGF) and human fibroblast growth factor 9 (FGF9) were from PeproTech (Rocky Hill, NJ). All inhibitors were from TOCRIS (Minneapolis, MN).