Uas rab7 gfp

W1118, GMR-Gal4(9146), long-GMR-Gal4(8121), UAS-rac1W(28874), rac1^J11 (6674), rac1^J11, rac2^Δ(6677), rac1^J10, rac2^Δ,, mtl^Δ,(6679), cdc42¹(7337), Rho1^E3.10(3176), UAS-rac1-RNAi(34910), UAS-YFP-rab21(23241), UAS-rab21-RNAi(29403), UAS-rab7-GFP(42706), UAS-rab7-RNAi(27051), vav^KG02022(14176), UAS-cdc42-RNAi-1#(29004), UAS-cdc42-RNAi-2#(37477), UAS-Rho1-RNAi-1#(9909), UAS-Rho1-RNAi-2#(29002), UAS-trio-RNAi-3#(27732), UAS-trio-RNAI-4#(43549), UAS-sos-RNAi-3#(31275), UAS-sos-RNAi-4#(34833), and UAS-vav-RNAi(39059) lines were provided by Bloomington Drosophila Stock Center, Bloomington, IN, USA. UAS-rac1-RNAi (v49246), UAS-egr-RNAI-1#(v45252), UAS-egr-RNAi-2#(v45253), UAS-trio-RNAi-1#(v40137), UAS-trio-RNAi-2#(v40138), UAS-sos-RNAi-1#(v42848), UAS-sos-RNAi-2#(v106925), UAS-dLRRK-RNAi-1#(v22139), and UAS-dLRRK-RNAi-2#(v22140) were provided by Vienna Drosophila RNAi Center, Vienna, Austria. UAS-egr was provided by Dr. Konrad Basler.^{6 (link)} sqh-PAK1^RBD-GFP was provided by Dr. Susan Parkhurst.^{22 (link)} TRE-GFP was provided by Dr. Dirk Bohmann.^{23 (link)} UAS-GFP-myc-2xFYVE was provided by Dr. Hugo Bellen (Baylor College of Medicine, Houston, TX, USA). UAS-dLRRK-WT, UAS-dLRRK-I1915T, and UAS-dLRRK-3KD were provided by Dr. Bingwei Lu.^{43 (link)}

Canton- S, UAS Actin GFP (III) (Bloomington 9257); UAS TubulinGFP (Bloomington 7373); UAS Rac1N17 (Bloomington 6292); UAS Cdc42N17 (Bloomington 6288); UAS RhoAN19 (Bloomington 7328); UAS snsRNAi; UAS dufRNAi, UAS jagunalRNAi (VDRC 108991), UAS tubulin RNAi (VDRC 33427), UAS DotGal4 (Bloomington 6903), UAS Rab5GFP, UAS Rab7GFP, UAS Rab11GFP (kind gift from Prof. Marcos Gonzalez Gaitan, University of Geneva), P{sqh-EYFP-Golgi}3 (Bloomington 7193).

The Sap-r^2-2 mutant was generated as described by Parks et al. (2004) (link) using the lines d00389 and e01294 from the Exelixis collection, Harvard. Mutant candidates were selected by white eye color and confirmed by genomic PCR and real-time RT-PCR. The stock was kept balanced with TM6B GFP, and homozygous animals were recognized by the absence of GFP fluorescence. Heterozygous animals were generated by crossing w¹¹¹⁸ virgins with Sap-r^2.2/TM6B-GFP balanced males and collecting non-GFP progeny.
UAS-Sap-r fly lines were created by cloning the Sap-r cDNA into pUAST-attB, and injection into flies with landing sites at 51C and 86F, respectively, was done by BestGene Inc., California. Other fly stocks used were act-Gal4, UAS Atg8a-mCherry, UAS Rab7-GFP, UAS Rab5-GFP and Df(3R) Exel 8194 [Bloomington Drosophila Stock Center (BDSC)]. Since the mutant parent strains were made in a w¹¹¹⁸ background, w¹¹¹⁸ were used as control animals. As a second control, we used wild-type Oregon R flies in some experiments (as indicated). The act-FRT-CD2-FRT-Gal4 clonal driver line (BDSC) to induce rescue clones was genomically recombined with Sap-r^2.2 using standard Drosophila techniques.