The sterols/steroids were purchased from Sigma Chemical (St. Louis, MO, USA) or Steraloids Inc. (Newport, RI, USA); the other diet constituents were purchased from Sigma Chemical (St. Louis, MO, USA). The purchased sterols were tested for purity by HPLC against standards: cholesterol, stigmasterol, cholestan-3-one, and cholest-4-en-3-one were >99% pure, and sitosterol (from Sigma Chemical), which was 60% pure, and was brought to >99% purity by HPLC. Chemically-defined diets were prepared as described previously [15] . Each diet contained 0.15 M amino acids and 0.5 M sucrose, and sterols/steroids were added following published methods [11] (link). Briefly, they were dissolved in chloroform (1 mg ml−1), and added to diets at concentrations between 0.1 and 10 µg sterol ml−1.
Cholestan 3 one
Manufactured by Merck Group
Sourced in China
Cholestan-3-one is a chemical compound that can be used as a laboratory reagent. It is a steroid with the molecular formula C27H46O. Cholestan-3-one is utilized in various analytical and research applications, but a detailed description of its core function is not available while maintaining an unbiased and factual approach.
Automatically generated - may contain errors
2 protocols using cholestan 3 one
1
Preparation of Defined Sterol Diets
2
Analytical Techniques for Sterol Determination
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cholest-4-en-3-one, cholest-5-en-3-one,
cholesterol, cholestan-3-one, and the solvents and reagents required
for TLC, HPLC, and GC–MS analyses were purchased from Sigma-Aldrich
(Shanghai, China). Cholest-4-en-3-ol and cholest-7-en-3-one were purchased
from Steraloids (Newport, RIUS;https://www.steraloids.com/contact-us ), and a methyltransferase colorimetric assay kit was purchased from
NeoBioscience Technology (Shenzhen, China).
cholesterol, cholestan-3-one, and the solvents and reagents required
for TLC, HPLC, and GC–MS analyses were purchased from Sigma-Aldrich
(Shanghai, China). Cholest-4-en-3-ol and cholest-7-en-3-one were purchased
from Steraloids (Newport, RIUS;
NeoBioscience Technology (Shenzhen, China).
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