Tween 20

Gold(III) chloride trihydrate (HAuCl₄·3H₂O, 99%), trisodium citrate dihydrate, potassium phosphate monobasic (KH₂PO₄) and bovine serum albumin (BSA) were purchased from Sigma-Aldrich. Sucrose, potassium carbonate (K₂CO₃), Tween 20, disodium hydrogen phosphate (Na₂HPO₃·12H₂O), and polyvinyl alcohol 1500 (PVA 1500) were purchased from Junsei Chemical Co., Ltd. (Tokyo, Japan). Affinity purified antibody against HBsAg, goat anti-mouse IgG, and recombinant HBsAg were purchased from Bore Da Biotech Co. Ltd. (Seongnam, Korea). Absorbent pad, backing card, nitrocellulose membrane (NC), sample pad, and conjugation pad were obtained from Bore Da Biotech Co. Ltd. (Seongnam, Korea).

Protein from the gastrocnemius muscle was extracted using radioimmunoprecipitation assay (RIPA) buffer. Protein concentrations of the supernatants were determined using a Pierce BCA Protein Assay Kit (Thermo Scientific, Rockford, IL, USA) and BSA as the standard. Total protein (10 μg per lane) was separated by 10% SDS−polyacrylamide gel electrophoresis and transferred to polyvinylidene difluoride membranes (Bio-Rad, Hercules, CA, USA). The membranes were blocked for 1 h at room temperature with tris-buffered saline containing 5% skim milk and 0.1% Tween 20 (Junsei, Tokyo, Japan). After overnight incubation at 4 °C with primary antibodies, the membranes were washed and incubated with a horseradish peroxidase-conjugated secondary antibody for 1 h at room temperature. Immunodetection was carried out with ECL Detection Reagent (Bio-Rad). All figures showing results of quantitative analysis using Image J software (National Institutes of Health, Bethesda, MD, USA) include data from at least three independent experiments.

Dimethyl sulfoxide (DMSO), ethylene glycol tetraacetic acid (EGTA), β-mercaptoethanol, CaCl₂, MgCl₂, NaOH, Triton X-100, ionomycin, NP-40, sodium deoxycholate, ammonium persulfate, KCl, formaldehyde, puromycin, penicillin, bromophenol blue, and ethanol were purchased from Sigma (USA). Phenylmethylsulfonyl fluoride (PMSF) was obtained from Bioworld (USA); and protease inhibitor cocktail, bovine serum albumin (BSA), and acrylamide solution were from GenDEPOT (USA). Sodium chloride (NaCl), ethylenediaminetetraacetic acid (EDTA), sodium dodecyl sulfate (SDS), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), and glycine were purchased from Duchefa (The Netherlands), and Tween 20 was obtained from Junsei Chemical Co., Ltd (Japan). Tris-HCl and glycerol were purchased from USB (USA).