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Mouse anti cyclin a

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Mouse anti-cyclin A is a primary antibody that specifically recognizes the cyclin A protein. Cyclin A is a key regulator of cell cycle progression, playing important roles in the transition from G1 to S phase and from G2 to M phase. This antibody can be used to detect and quantify cyclin A expression in various cell and tissue samples.

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Lab products found in correlation

Mouse anti γh2ax, by Merck Group (2 mentions) Mouse anti brdu fitc, by BD (2 mentions) Goat anti rrm2, by Santa Cruz Biotechnology (2 mentions) Rabbit anti c myc, by Cell Signaling Technology (2 mentions) Mouse anti β actin, by Merck Group (1 mentions) Rabbit anti p21, by Abcam (1 mentions) Mouse anti phospho atm, by Rockland Immunochemicals (1 mentions) Rabbit anti 53bp1, by Fortis Life Sciences (1 mentions) Mouse anti ki67, by Agilent Technologies (1 mentions) Ultravision quanto detection system hrp dab, by Thermo Fisher Scientific (1 mentions) Mouse anti gapdh, by Merck Group (1 mentions) Rabbit anti phospho erk1 2, by Cell Signaling Technology (1 mentions) Plx4720, by Plexxikon (1 mentions) Alliance 4.7 imaging system, by Uvitec (1 mentions) Protran ba83, by Cytiva (1 mentions) Mouse anti p53 do 1, by Santa Cruz Biotechnology (1 mentions) Rabbit anti cyclin d1, by Santa Cruz Biotechnology (1 mentions) Mouse anti cyclin b1, by Santa Cruz Biotechnology (1 mentions) Whatman, by Cytiva (1 mentions) Protran, by Cytiva (1 mentions)

Spelling variants of this product

Cyclin A (3 citations)

4 protocols using mouse anti cyclin a

1

Comprehensive Knockdown Assay Protocol

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All chemicals and reagents were purchased from Sigma-Aldrich (St. Louis, MO). pLKO.1-shRNA plasmids were obtained from Open Biosystems (Waltham, MA). The mature sense sequences are: shRRM2: 5′-CGGAGGAGAGAGTAAGAGAAA-3′; shATM #1: 5′-CGTGTCTTAATGAGACTACAA-3′; shATM #2: 5′-TGATGGTCTTAAGGAACATCT-3′; shp53: 5′-GAGGGATGTTTGGGAGATGTA-3′; and shc-MYC: 5′-CCTGAGACAGATCAGCAACAA-3. The following antibodies were obtained from the indicated suppliers: mouse anti-phospho-ATM (Rockland, Gilbertsville, PA), goat anti-ATM (Bethyl, Montgomery, TX), goat anti-RRM2 (Santa Cruz Biotechnology), mouse anti-Cyclin A (Novocastra), mouse anti-γH2AX (Millipore, Billerica, MA), rabbit anti-53BP1 (Bethyl), mouse anti-BrdU FITC (BD Biosciences, San Jose, CA), mouse anti-p53 (Calbiochem, Billerica, MA), rabbit anti-G6PD (Sigma-Aldrich), rabbit anti-p21 (Abcam, Cambridge, MA), rabbit anti-c-MYC (Cell Signaling, Danvers, MA), and mouse anti-β-actin (Sigma-Aldrich).
Aird K.M., Worth A.J., Snyder N.W., Lee J.V., Sivanand S., Liu Q., Blair I.A., Wellen K.E, & Zhang R. (2015). ATM couples replication stress and metabolic reprogramming during cellular senescence. Cell reports, 11(6), 893-901.
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2

Immunohistochemical Analysis of Cell Markers

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Paraffin‐embedded sections (3–5 μm) were used for IHC. Heat‐induced antigen retrieval was performed in Tris‐EDTA (pH 9) or citrate buffer (pH 6) before applying primary antibodies: mouse anti‐cytokeratin 10 (1:100, DAKO), rabbit anti‐cleaved Notch1 ICD (1:100, CST), mouse anti‐cyclin A (1:100, Novocastra Laboratories), and mouse anti‐Ki67 (1:100, DAKO). IHC was performed using the SuperBoost Tyramide kit (Thermo Fisher Scientific) or the Ultra Vision Quanto Detection System HRP DAB (Thermo Scientific) according to the manufacturers' protocols. Sections were mounted with ProLong Gold Antifade Reagent with DAPI (Molecular Probes, Thermo Scientific, EU) or Tissue‐Tek Compound (Sakura).
Bagdonaite I., Pallesen E.M., Ye Z., Vakhrushev S.Y., Marinova I.N., Nielsen M.I., Kramer S.H., Pedersen S.F., Joshi H.J., Bennett E.P., Dabelsteen S, & Wandall H.H. (2020). O‐glycan initiation directs distinct biological pathways and controls epithelial differentiation. EMBO Reports, 21(6), e48885.
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3

Vemurafenib Analog PLX4720 Inhibition

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The vemurafenib analog PLX4720 was provided by Plexxikon (Berkeley, CA). pLKO.1-shRRM2 plasmids were obtained from Open Biosystems (Waltham, MA). The following antibodies were obtained from the indicated suppliers: goat anti-RRM2 (Santa Cruz Biotechnology, Santa Cruz, CA), mouse anti-cyclin A (Novocastra Laboratories, Buffalo Grove, IL), mouse anti-γH2AX (Millipore), rabbit anti-cleaved lamin A (Cell Signaling, Danvers, MA), rabbit anti-c-Myc (Cell Signaling), rabbit anti-PARP p85 (Promega Corporation, Fitchburg, WI), rabbit anti-phospho-ERK1/2 (Cell Signaling), mouse anti-GAPDH (Millipore), rabbit anti-pS10H3 (Millipore), and mouse anti-BrdU FITC (BD Biosciences).
Fatkhutdinov N., Sproesser K., Krepler C., Liu Q., Brafford P.A., Herlyn M., Aird K.M, & Zhang R. (2016). Targeting RRM2 and Mutant BRAF is a Novel Combinatorial Strategy for Melanoma. Molecular cancer research : MCR, 14(9), 767-775.
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4

Western Blot Analysis of Cell Cycle Proteins

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The 30 μg of lysate was initially denatured in 4 × Loading Buffer (40% glycerol, 240 mM Tris-HCl pH 6.8, 8% SDS, 0.04% bromophenol blue, 5% β-mercaptoethanol) at 96°C for 5 min and separated on 8%, 10% or 12% SDS-polyacrylamide gels and transferred to nitrocellulose membranes (Whatman Protran BA 83; 0.2 µm pores) and blocked with 4% skimmed milk in a TBS-Tween buffer (TBST) for 20 min. Primary antibodies in this study are as follows: mouse anti-cyclin A (Novocastra, UK), mouse anti-cyclin B1 (Santa Cruz Biotechnology, USA), rabbit anti-cyclin D1 (Santa Cruz Biotechnology, USA), mouse anti-cyclin E1 (Santa Cruz Biotechnology, USA), mouse anti-p53 DO1 (Santa Cruz Biotechnology, USA). After incubation with appropriate horseradish peroxidaseconjugated secondary antibody, proteins were visualized by using Western Lightning Plus-ECL Enhanced Chemiluminiscence Substrate (Perkin Elmer, USA). Western blots were imaged using an Alliance 4.7 imaging system (UVitec, UK).
Hanžić N., Horvat A., Bibić J., Unfried K., Jurkin T., Dražić G., Marijanović I., Slade N, & Gotić M. (2018). Syntheses of gold nanoparticles and their impact on the cell cycle in breast cancer cells subjected to megavoltage X-ray irradiation. Materials science & engineering. C, Materials for biological applications, 91.
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