To determine the suitability of the produced substrates, they were characterized with regard to their nanoscale roughness, transmittance, surface wettability, and stiffness. A stylus profilometer (
Dektak 150, Veeco Instruments) was used to obtain the topographical cross section of textured scaffolds and the size of features.
To evaluate the topography and quantify the roughness of substrates, atomic force microscope (AFM) (
NTEGRA, NT‐MDT, Russia) was used, in semicontact mode at an average set point of 10. Golden silicon probes (NSG01 and NSG10; obtained from NT‐MDT) were used, with an average force constant of 14.3 N/m. Root mean square (RMS) roughness was obtained by the software associated with the AFM (NOVA P8 Spectra).
The transmittance of substrates under study was recorded at 550 nm using a UV‐visible light spectrophotometer (
UV‐2501PC, Shimadzu). Wettability of the substrates was measured using drop shape analysis method (
DSA25B, Krüss). The static sessile‐drop contact angle between DI water and the substrate was recorded by the ADVANCE software.
Elídóttir K.L., Scott L., Lewis R, & Jurewicz I. (2022). Biomimetic approach to articular cartilage tissue engineering using carbon nanotube–coated and textured polydimethylsiloxane scaffolds. Annals of the New York Academy of Sciences, 1513(1), 48-64.