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Dsa25b

Manufactured by Krüss
Sourced in Germany

The DSA25B is a piece of lab equipment manufactured by Krüss. It is a drop shape analysis system designed to measure the surface tension, contact angle, and other wetting properties of liquids on solid surfaces.

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6 protocols using dsa25b

1

Wettability Characterization of 2T Films

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To characterize the wettability of 2T films obtained from the oil/water
interface, the water contact angle was measured using a contact angle
analyzer (Krüss, model DSA25B) at the room temperature of 21
± 1 °C. 2T films were transferred from the oil/water onto
a square piece of silicon wafer by two different methods: (1) pushing
a wafer downward onto a floating film to produce a 2T surface presenting
the water side and (2) lifting the wafer upward from under a floating
film to produce a 2T surface presenting the oil side. On each sample,
three 4 μL water droplets were deposited in different areas
to acquire the mean water contact angle.
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2

Surface Wettability of Abraded Dentin

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The static contact angle (CA) and the surface free energy (SFE) were measured for five samples from each group (A, B, and C). The test was performed using a goniometer (DSA 25B, Krüss GmbH, Hamburg, Germany). The contact angles of samples in air and controlled environment (temperature, 22 °C; humidity, 40%) were measured for three types of test liquids (1-bromonaphthalene, diiodomethane, and deionized water) applied to the prepared dentin. Samples were kept in environmental conditions of 22 °C and 40% humidity for 24 h before the measurement to minimize the variability in materials’ surface properties. Digital measurements were performed with a 10 μL droplet using a syringe equipped with a needle placed every 10 s (10 droplets per sample). The surface free energy was calculated based on the results of the contact angles using the distilled water calculation method according to the equation proposed by Robeson [43 ]:
and using 3 test liquids (Owens−Wendt−Rabel−Kaelble (O-W-R-K) method). This procedure was applied to provide information on possible changes in the contact angles and the surface free energy of the dentine subjected to the abrasive blasting method in the form of air microabrasion.
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3

Water Contact Angle Measurement

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Water contact angle was measured through the sessile drop method on a goniometer (Kruss, model DSA25B, Hamburg, Germany). Distilled water drops (11 µL in volume) were cast on the surface of five samples (20 × 20 mm2) per treatment and the contact angle was measured every 10 s for 60 s.
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4

Characterization of Nanoscale Scaffold Properties

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To determine the suitability of the produced substrates, they were characterized with regard to their nanoscale roughness, transmittance, surface wettability, and stiffness. A stylus profilometer (Dektak 150, Veeco Instruments) was used to obtain the topographical cross section of textured scaffolds and the size of features.
To evaluate the topography and quantify the roughness of substrates, atomic force microscope (AFM) (NTEGRA, NT‐MDT, Russia) was used, in semicontact mode at an average set point of 10. Golden silicon probes (NSG01 and NSG10; obtained from NT‐MDT) were used, with an average force constant of 14.3 N/m. Root mean square (RMS) roughness was obtained by the software associated with the AFM (NOVA P8 Spectra).
The transmittance of substrates under study was recorded at 550 nm using a UV‐visible light spectrophotometer (UV‐2501PC, Shimadzu). Wettability of the substrates was measured using drop shape analysis method (DSA25B, Krüss). The static sessile‐drop contact angle between DI water and the substrate was recorded by the ADVANCE software.
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5

Surface Wettability Evaluation Utilizing Sessile Drop

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After measuring surface roughness parameters, the same samples were used to measure the surface wettability adopting sessile drop method using deionized water by the aid of a contact angle measuring device (DSA25B, Krüss GmbH, Germany). For each sample, 3 readings were recorded for 3 different drops and the average value was considered the mean reading for every tested sample. All mean values were reported for statistical analysis.
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6

Evaluating Membrane Hydrophilicity via Water Contact Angle

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To evaluate the surface hydrophilicity of the membranes, static water contact angle experiments were conducted using a contact angle goniometer (DSA25B, Krüss, Hamburg, Germany). The wettability was tested using double-deionized water with a conductivity of 0.09 μS/cm according to standard PN-EN ISO3696:1999 (HLP20 UV, Hydrolab, Straszyn, Poland). Analyses were performed by observing the shape of liquid droplets placed on the sample and directly measuring the angle at the point of contact with the surface. Measurements were repeated three times for each sample type in air under controlled conditions (25 °C, 45% humidity) using a 5 mL glass syringe equipped with a flat-tip needle. Droplets (5 μL volume) were placed on the surface every 10 s (10 droplets per sample). The shape of the droplets was recorded directly using a digital camera and processed using the Krüss ADVANCE computer program (Krüss, Hamburg, Germany).
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