siRNAs against Ago2, miR-30a mimic and miR-30a inhibitor were purchased from RiboBio Co., Ltd. (Guangzhou, China) and transfected into COV434 cells at a concentration of 200 nM.
Mir 30a inhibitor
Manufactured by RiboBio
Sourced in China
The MiR-30a inhibitor is a lab equipment product designed to inhibit the expression of the miR-30a microRNA. It functions by binding to and blocking the miR-30a molecule, preventing it from carrying out its regulatory activities within the cell.
Automatically generated - may contain errors
3 protocols using mir 30a inhibitor
1
Silencing Ago2 and Modulating miR-30a
2
miR-30a Transfection Protocols
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A miR-30a mimic, miR-30a inhibitor, and negative control mimic were purchased from Ribobio (Guangzhou, China). Lentiviruses used to knockdown or overexpress human miR-30a, in addition to negative control Lentiviruses, were purchased from Hanbio (Shanghai, China). All transfections were carried out according to the manufacturer’s instructions.
3
Modulating LSINCT5 Expression in MCF-7 Cells
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The short hairpin RNA (shRNA) specifically targeting LSINCT5 (called sh-LSINCT5) was synthesized by GenePharma (Shanghai, China), and nonspecific shRNA was used as negative control (sh-NC). The full-length sequence of LSINCT5 was inserted into pcDNA3.1 vector (pcDNA-LSINCT5, Invitrogen, Grand Island, NY, USA) and an empty pcDNA vector as negative control. The miR-30a mimic, miR-30a inhibitor, and corresponding NC were synthesized by RiboBio (Guangzhou, China). According to the manufacturer’s protocol, MCF-7 cells was stably transfected with all plasmids for 48 hours using Lipofectamine 3000 reagent (Invitrogen, Grand Island, NY, USA).
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