Advia centaur xpi

Blood collection took place between 8:00 and 10:00 am, and serum was extracted from the samples. LH was measured with CLIA using the automated platform Advia-Centaur XPi® (Siemens Inc., NY). The lowest level of quantification (LLOQ) was 0.12 U/L, and according to the manufacturer the intra-assay coefficient of variation ranged between 2,3% and 3.0%, and the inter-assay coefficient of variation between 1,5% and 2.9%. Testosterone was also measured with CLIA, using the automated platform Advia-Centaur XPi® (Siemens Inc., NY). The LLOQ was 10 ng/dL, and according to the manufacturer, the intra-assay coefficient of variation ranged between 2.3% and 6.2%, and the inter-assay coefficient of variation between 2.7% and 6.9% (Manufacturer information: 10629910_ES Rev. U, 2014–08, 1–18). Testosterone was also measured with LC MSMS in the validation cohort. An ultra high-pressure liquid chromatography with the 1290 Infinity Binary LC System (Agilent Technologies, Santa, CA) was performed. The system is connected in parallel to a tandem mass spectrometry using the 6430 Series Tripe Quadruple LC/MS System (Agilent Technologies, Santa Clara, CA). The LLOQ was 2 ng/dL and thee intra-assay coefficient of variation ranged from 4% to 5% and the inter-assay coefficient of variation between 7% and 8%, at levels of ST below 50 ng/dL [13 (link)].