Liquid chopped meat broth

Salmonella enterica subsp enterica serovar Typhimurium (ATCC 35986) was grown under aerobic conditions at 37°C for 1–2 d on LB agar plates (Sigma-Aldrich). Growth of anaerobic bacteria was performed using a BD GasPak EZ Anaerobe Pouch System (BD Diagnostics). Prevotella stercorea (DSM No. 18206) was grown for 5–7 d under anaerobic conditions in liquid-chopped meat broth (Hardy Diagnostics) supplemented with 1% Trace Minerals (ATCC), 1% Vitamin Supplements (ATCC), 0.05% Tween 80, 29.7mM acetic acid, 8.1 mM propionic acid and 4.4 mM butyric acid (Sigma-Aldrich). Ruminococcus bromii (ATCC# 27255) was grown under anaerobic conditions at 37°C for 1–2 d in liquid-chopped meat broth (Hardy Diagnostics). The long-term stock of Bifidobacterium longum subsp infantis (ATCC 15697) was grown under anaerobic conditions at 37°C for 2–3 d in liquid-chopped meat broth (Hardy Diagnostics) and the working stock was grown on Brucella plates (Teknova) under anaerobic conditions at 37°C for 2–3 d. Acinetobacter junii (ATCC 17908) was grown under aerobic conditions at 26°C for 1–2 d using Nutrient Agar plates (Edge Biologicals). Single-use working stocks of all bacteria were prepared using DPBS and long-term stocks were prepared using 10% glycerol. All bacterial stocks were enumerated using the BD Cell Viability Kit (BD Bioscience) and stored at −80°C until use.