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May gr nwald giemsa solution

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May-Grünwald Giemsa solutions are a set of staining reagents used in microscopy for the differentiation and identification of cellular components. These solutions are commonly used in hematology, cytology, and histology to stain blood smears and other biological samples. The solutions contain a mixture of dyes, including May-Grünwald and Giemsa, which selectively stain various cellular structures, enabling visualization and analysis under a microscope.

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Lab products found in correlation

Ab9535, by Abcam (1 mentions) Ab64264, by Abcam (1 mentions) Nanozoomer digital slide scanner, by Hamamatsu Photonics (1 mentions) Abx micros abc vet equipment, by Horiba (1 mentions) Ethylene diamine tetraacetic acid (edta), by Merck Group (1 mentions) Cytospin centrifuge, by Thermo Fisher Scientific (1 mentions) Prolong gold with dapi, by Thermo Fisher Scientific (1 mentions) Trypsinised, by Thermo Fisher Scientific (1 mentions) Fetal calf serum (fcs), by Thermo Fisher Scientific (1 mentions) Ficoll paque gradient, by GE Healthcare (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Merck Group (1 mentions) S monovette, by Sarstedt (1 mentions) Cytospin centrifugation, by Thermo Fisher Scientific (1 mentions) Dpx mountant for histology, by Merck Group (1 mentions)

Close spelling variants of this product

May–Grünwald and Giemsa solutions May-Grünwald and Giemsa stain solution May-Grünwald-Giemsa May-Grünwald solution May-Grünwald Giemsa solution Giemsa

8 protocols using may gr nwald giemsa solution

1

Comprehensive Hematological and Histological Analysis

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Blood was collected from tail veins into ethylenediaminetetraacetic acid (EDTA)-coated tubes. Automated peripheral blood counts were obtained using a Vet abc machine (scil). Blood smears and bone marrow cytospins were stained with May-Grünwald Giemsa solutions (Sigma). Mouse tissues were fixed in formalin and embedded in paraffin. Blocks were cut into 5 μm sections and mounted onto glass slides prior to staining with hematoxylin and eosin. For immunohistochemistry, slides were microwaved in 10 mM citric acid (pH 6.0) to retrieve antigens. Endogenous peroxidases were quenched using hydrogen peroxide and staining was performed using anti-myeloperoxidase antibody (1:100, ab9535, Abcam) diluted in TBST for 1 h at room temperature. Signal detection was accomplished using an HRP/DAB (ABC) Detection IHC kit (Abcam, ab64264). Sections were counterstained with hematoxylin before dehydration and coverslip mounting. Slide images were captured using a Nanozoomer digital slide scanner (Hamamatsu).
Supper E., Rudat S., Iyer V., Droop A., Wong K., Spinella J.F., Thomas P., Sauvageau G., Adams D.J, & Wong C.C. (2021). Cut-like homeobox 1 (CUX1) tumor suppressor gene haploinsufficiency induces apoptosis evasion to sustain myeloid leukemia. Nature Communications, 12, 2482.
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2

Blood Cell Analysis Protocol

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Blood samples were collected in tubes with EDTA as an anticoagulant (Sigma Chemical Co., USA), and a blood cell count was performed on ABX Micros ABC Vet equipment (Horiba ABX, France). Morphological and leucocyte differentiation analyses were performed on blood smears stained with standard May–Grünwald–Giemsa solutions (Sigma Chemical Co., USA).
Barbosa C.M., Fock R.A., Hastreiter A.A., Reutelingsperger C., Perretti M., Paredes-Gamero E.J, & Farsky S.H. (2019). Extracellular annexin-A1 promotes myeloid/granulocytic differentiation of hematopoietic stem/progenitor cells via the Ca2+/MAPK signalling transduction pathway. Cell Death Discovery, 5, 135.
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3

Evaluating Cellular Morphology Changes

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To investigate potential macroscopic modifications of cell morphology, cells (primary and cell lines) were seeded in 6-well plates at 0.5 × 105 cells/ml and incubated with increasing concentration of AZD-1775 for 24 h. Cells were harvested, spun down (10 min at 200 g) onto glass slips using a Cytospin™ centrifuge (Thermofisher), and then stained with the May-Grünwald Giemsa solutions (Sigma-Aldrich). The slides were analyzed using an optical microscope AXIOVERT 40 CFL and the pictures analyzed using AxioVision Rel.4.7 software. For the immunofluorescence analysis, BV-173 cells were seeded to poly-d-lysine-coated coverslips, fixed with 4% paraformaldehyde (PFA) and stained at 37 °C with an anti-phospho-Ser/Thr-Pro MPM-2 antibody FITC conjugated (Millipore Sigma). Coverslips were, then, mounted on glass slides using a mounting media with DAPI (4′,6-diamidino-2-phenylindole) (Prolong Gold with DAPI, Invitrogen). Immunofluorescence analyses were performed using the AXIOVERT 40 CFL microscope and the picture analyzed using AxioVision Rel.4.7 software.
Ghelli Luserna Di Rorà A., Beeharry N., Imbrogno E., Ferrari A., Robustelli V., Righi S., Sabattini E., Verga Falzacappa M.V., Ronchini C., Testoni N., Baldazzi C., Papayannidis C., Abbenante M.C., Marconi G., Paolini S., Parisi S., Sartor C., Fontana M.C., De Matteis S., Iacobucci I., Pelicci P.G., Cavo M., Yen T.J, & Martinelli G. (2018). Targeting WEE1 to enhance conventional therapies for acute lymphoblastic leukemia. Journal of Hematology & Oncology, 11, 99.
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4

Cell Smear Staining and Imaging

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The cell pellet of MLACs, TAMs, Mo-MDSCs, or PMN-MDSCs (1.0 × 104 cells each) were smeared onto glass slides and air-dried. The smear was then stained with May-Grünwald/Giemsa solutions (Sigma Aldrich, Missouri, USA) as described by the manufacturer's protocol and photos were taken under light microscopy.
Tsubaki T., Kadonosono T., Sakurai S., Shiozawa T., Goto T., Sakai S., Kuchimaru T., Sakamoto T., Watanabe H., Kondoh G, & Kizaka-Kondoh S. (2018). Novel adherent CD11b+ Gr-1+ tumor-infiltrating cells initiate an immunosuppressive tumor microenvironment. Oncotarget, 9(13), 11209-11226.
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5

Morphological Analysis of Sorted Cells

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To evaluate the morphology of cells, 1 × 105 sorted Ly6G+ and Ly6C+ cells were fixed on glass slides using the cytospin technique. Then, the cells were dried and stained with May-Grünwald/Giemsa solutions (Sigma-Aldrich, St. Louis, MO, USA) according to the standard procedure. The stained cells were then observed under a light microscope (Olympus, Prague, Czech Republic), and an analysis of the cell types was done at 1000× magnification.
Mačak Kubašková T., Mudroňová D., Vargová M., Reiterová K, & Hrčková G. (2021). Cellular and humoral peritoneal immunity to Mesocestoides vogae metacestode infection in mice. Parasites & Vectors, 14, 54.
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6

Monocyte Migration Assay Protocol

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Human monocytes were isolated as described previously(18 (link), 20 , 29 (link)). Briefly, monocytes were isolated by centrifugation of citrate-phosphate-dextrose-adenine (CPDA)-coagulated blood (S-Monovettes, Sarstedt, Nümbrecht, Germany) on a Ficoll-Paque gradient (GE Healthcare). Leukocytes were cultured overnight at 37°C, 5% CO2 in a cell culture flask in RPMI 1640 (Gibco, Carlsbad, CA, USA) supplemented with 10% FCS (Gibco) and 1% Penicillin/Streptomycin (Sigma-Aldrich, St. Louis, MO, USA). For the migration assay, non-adherent leukocytes were removed and adherent cells were trypsinised (Gibco), centrifuged, and resuspended in medium. Monocyte migration was performed in a modified 48-well Boyden chamber (NeuroProbe Inc., Geithersburg, MD, USA), using a 5 μm pore filter to separate the 2 compartments. Monocytes were added in the upper compartment and 20 μg/ml S100A9 (abcam) or 200 nmol/L CyPA (R&D Systems) or CyPA together with 20 μg/ml of the potential antibodies or rIgG2a (Invitrogen, Carlsbad, CA, USA) were added in the lower compartment. The receptor blocking antibody αCD147 and mIgG1 were added to the monocytes into the upper compartment. After 4 hours at 37°C, 5% CO2 the filter was fixed in Methanol, stained in a May-Grünwald/ Giemsa solution (Merck Millipore, Darmstadt, Germany) and the migrated cells on the filter were counted using a microscope.
von Ungern-Sternberg S.N., Vogel S., Walker-Allgaier B., Geue S., Maurer A., Wild A.M., Münzer P., Chatterjee M., Heinzmann D., Kremmer E., Borst O., Loughran P., Zernecke A., Neal M.D., Billiar T.R., Gawaz M, & Seizer P. (2017). Extracellular cyclophilin A augments platelet-dependent thrombosis and thrombo-inflammation. Thrombosis and haemostasis, 117(11), 2063-2078.
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7

Cell Culture and Transfection Protocols

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293T human embryonic kidney cells, human cancer cell lines (U-87, MCF-7, HeLa, 293T, AN3CA, Mia Paca-2, PANC-1), and early-passage (p3) mouse embryonic fibroblasts (MEFs) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with fetal bovine serum (FBS) and antibiotics as described10 (link). Transfection was carried out by the calcium phosphate-DNA precipitation method37 (link). CML-derived K562 cells were maintained and the growth curve analysis was performed as described10 (link). Where indicated, cells were washed in PBS, and re-plated in fresh medium without curcumin and PGV-1. Viable and dead cells were counted using the trypan blue exclusion method. In some cases, cells were fixed on slide glass by cytospin and inspected after staining with May-Grünwald Giemsa solution (Merck).
Lestari B., Nakamae I., Yoneda-Kato N., Morimoto T., Kanaya S., Yokoyama T., Shionyu M., Shirai T., Meiyanto E, & Kato J.Y. (2019). Pentagamavunon-1 (PGV-1) inhibits ROS metabolic enzymes and suppresses tumor cell growth by inducing M phase (prometaphase) arrest and cell senescence. Scientific Reports, 9, 14867.
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8

Multiparametric Cell Analysis Protocol

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Cells were immobilized on glass slides at a concentration by cytospin centrifugation (ThermoFisher Scientific) at 300 rpm for 10 min. Once dried, cells were stained with May-Grünwald Giemsa solution (Merck, New Jersey). Cells were further stained with Myeloperoxidase, alpha-naphthyl butyrate esterase and alpha-naphthyl acetate esterase. Cells were mounted with DPX Mountant for histology (Sigma-Aldrich, Misuri) and examined using the microscope Olympus AX70 TRF.
Maher M., Diesch J., Le Pannérer M.M., Cabezón M., Mallo M., Vergara S., Méndez López A., Mesa Tudel A., Solé F., Sorigue M., Zamora L., Granada I, & Buschbeck M. (2021). Divergent leukaemia subclones as cellular models for testing vulnerabilities associated with gains in chromosomes 7, 8 or 18. Scientific Reports, 11, 21145.
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