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9 protocols using chloroplatinic acid hexahydrate

1

Analytical Reagents Characterization

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In this study, all chemicals were of analytical grade and used without further purification. Chloroplatinic acid hexahydrate and 2,4-dinitrotoluene were supplied from Sinopharm Chemical Reagent Co., Ltd. Nitrobenzene was purchased from Tianjin Fuchen Chemical Reagent Co., Ltd. o-Nitrotoluene, m-nitrotoluene and p-nitrotoluene were purchased from Shanghai Aladdin Reagent Co., Ltd. 4-Nitroanisole was supplied by TCI (Shanghai) Chemical Industry Development Co., Ltd. Ethanol was supplied by Tianjin Chemical Reagent Third Factory. The water used was distilled water obtained from an Ulupure UPR-II system.
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2

Synthesis of Graphene-Based Materials

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Graphite flakes, sodium chloride (NaCl, ≥99.0%), sulfuric acid (H2SO4, 98%), sodium nitrate (NaNO3, ≥99%), potassium permanganate (KMnO4, ≥99%), hydrogen peroxide (H2O2, 30% in water), concentrated aqueous solution hydrochloric acid (HCl, 37 wt.%), copper(II) nitrate hydrate (Cu(NO3)2·3H2O, ≥99%), chloroplatinic acid hexahydrate (H2PtCl6·6H2O, 99.9%), palladium chloride (PdCl2, 99%), ethylene glycol (EG, 99%), and triethylene glycol (TEG, 99%) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Beijing, China). All chemicals were used as received without further purification. Deionized (DI) water was used in this experiment to prepare the solutions.
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3

Adsorption and Catalysis Reagents Protocol

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All the chemicals in the experiments were of analytical reagent grade and used without further purification. Ammonium molybdate ((NH4)6Mo7O24·4H2O), hexamethylenetetramine (C6H12N4), active charcoal powder, cadmium nitrate tetrahydrate (Cd(NO3)2·4H2O), and Na2S, chloroplatinic acid hexahydrate (H2PtCl6·6H2O) were purchased from Sinopharm Chemical Reagent Co., Ltd, China.
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4

Synthesis of Pt-Bi Nanoalloy Catalysts

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Chloroplatinic acid hexahydrate (H2PtCl6·6H2O, 99.9%), bismuth nitrate (Bi(NO3)3·5H2O, 99.0%), ethylene glycol (EG, 99%), polyvinylpyrrolidone (PVP, K30, 99.9%) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Beijing, China). The borane–ammonia complex (H3N·BH3, 90%), commercial Pt/C (Pt/C, 20 wt%), and Nafion 117 solution (5%) were purchased from Sigma Aldrich (St. Louis, MO, USA). Ultrapure water (18.2 MΩ cm) was used in the experiment.
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5

Synthesis of Pt-SnO2 Nanocomposites

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All chemicals were utilized as received without further purification. Chloroplatinic acid hexahydrate [H2PtCl6·6H2O], tin(ii) chloride dehydrate [SnCl2·2H2O], urea [CO(NH2)2] and ethylene glycol (EG) were of analytical reagent (A.R.) grade and purchased from Sinopharm Chemical Reagent Co., Ltd. Commercial 30 wt% Pt/C was purchased from Johnson Matthey. Deionized water (DI water, Millipore, 18.2 MΩ at 25 °C) was used in all processes.
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6

Synthesis of Functionalized Nanoparticles

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All chemicals were analytical grade and used without further purification. Hexadecyltrimethylammonium bromide (C19H42NBr, CTAB), silver nitrate (AgNO3), sodium borohydride (NaBH4), l-ascorbic acid (C6H8O6, AA), hydrochloric acid (HCl, 37 wt%) and benzotrifluoride (C7H5F3, BTF) were purchased from Sigma Aldrich. Benzyl alcohol (C7H8O, BA), benzaldehyde (C7H6O, BAD) and sodium oleate (C18H33O2Na, NaOL) were purchased from Aladdin. 4-Methylbenzyl alcohol (C8H10O), 4-methoxybenzyl alcohol (C8H10O2), 4-chlorobenzyl alcohol (C7H7ClO), and 4-hydroxybenzyl alcohol (C7H8O2) were supplied by Alfa Aesar. Cadmium acetate (C4H6O4Cd, Cd(Ac)2), thioacetamide (C2H5SN, TAA), chloroauric acid tetrahydrate (HAuCl4·4H2O), glycine acid (C2H5NO2, Gly), anhydrous ethanol (C2H5OH, EtOH) and chloroplatinic acid hexahydrate (H2PtCl6·6H2O) were obtained from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). Deionized water used in the synthesis was obtained from local sources.
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7

Facile Synthesis of Metal Nanoparticles

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Chloroplatinic acid hexahydrate (H2PtCl6·6H2O), copper nitrate trihydrate [Cu(NO3)2·3H2O], cerium(iii) nitrate hexahydrate [Ce(NO3)3·6H2O], sodium hydroxide (NaOH), sodium borohydride (NaBH4), and glycerol were of analytical reagent (A.R.) grade and purchased from Sinopharm Chemical Reagent Co., Ltd. All chemicals were used as received. Deionized water (DI water, Millipore, 18.2 MΩ at 25 °C) were used in all processes.
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8

Biomimetic Nanomaterials for Biosensing

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Chloroauric acid (HAuCl4·4H2O), chloroplatinic acid hexahydrate (H2PtCl6·6H2O), silver nitrate, copper sulfate pentahydrate (CuSO4·5H2O), NaOH, KNO3, KCl, hemin, adenosine triphosphoric acid (ATP) and sodium pyrophosphate were obtained from Sinopharm Chemical Reagent Co. (Shanghai, China).
Guanine (G)-rich 5′-GGGTAGGGCGGGTTGGG-3′ (G-rich ODN) and Guanosine (G)-free 5′-AAATAAAACAAATTAAA-3′ (G-free ODN, for control experiment) were obtained from Sangon Biotechnology Co. Ltd. (Shanghai, China) and dissolved or diluted in sterile deionized water. The S1 nuclease was achieved from TaKaRa Biotechnology (Dalian, China). The S1 nuclease buffer (2 mM NaAc, 15 mM NaCl, and 0.1 mM ZnSO4, pH 4.5) was used for S1 dilution and enzymatic digestion reaction. All other chemicals were of analytical grade and used without further treatment. Deionized water was prepared using a Millipore Milli-Q water purification system (18.2 MO). Fresh hen eggs were bought from local market without special selection and the egg white was taken from them by drawing.
Escherichia coli (E. coli) cell medium were obtained friendly.
The fluorescence spectra were recorded on a fluorescence spectrophotometer (Hitachi, F-4600). Zeta potential measurements were carried out on a Malvern Zetasizer Nano ZS. The circular dichroism (CD) spectra were recorded on a Jasco 815 spectropolarimeter.
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9

Synthesis of Metal-Doped Silicon Nanostructures

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Copper chloride dihydrate (CuCl2•2H2O, 99%), ethanol (C2H5OH, 99%), acetone (C3H6O, 99.5%) and chloroplatinic acid hexahydrate (H2PtCl6•6H2O with Pt content ≥ 37%) were purchased from Sinopharm Chemical Reagent Co., Ltd., China. Cobalt chloride hexahydrate (CoCl2•6H2O, 99.7%), sodium borohydride (NaBH4, 98%), urea (CH4N2O, 99%) and hydrofluoric acid (HF, ≥ 40%) were purchased from Aladdin Reagents, Shanghai, China. All reagents were used without further purification. The silicon wafers were purchased from Zhejiang Lijing Silicon Material Co., Ltd. China., and cleaned with acetone, ethanol, and then the deionized water for three times before the usage.
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