To determine total Cd concentration in the soil samples, 0.5 g air dried sample was digested with 7 mL nitric acid and 3 mL hydrofluoric acid in a polytetrafluoroethylene digestion vessel using a microwave accelerated reaction instrument (CEM-MARS6 Xpress, United States). After complete digestion, the total, water-soluble and filter solution of all samples were measured with a 7,700× Inductively Coupled Plasma Mass Spectrometer (Agilent Technologies, Japan). A heavy metal uncontaminated soil sample (GSS-7, shown in Table 1 ) was used as a standard negative control.
7 700 inductively coupled plasma mass spectrometer
Manufactured by Agilent Technologies
Sourced in Japan
The 7,700× Inductively Coupled Plasma Mass Spectrometer is a high-performance analytical instrument designed for the detection and quantification of a wide range of elements in various sample types. It utilizes inductively coupled plasma technology to efficiently ionize and introduce samples into a mass spectrometer, allowing for accurate and sensitive measurements of elemental composition.
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Lab products found in correlation
4 protocols using 7 700 inductively coupled plasma mass spectrometer
1
Determination of Total Cadmium in Soil
2
Manganese Nanoparticle Uptake Quantification
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MC38 cells were treated with Mn‐N/C (100 µg mL−1) plus with H2O2 (100 µm ) for 24 h. After that, the culture medium was collected and centrifugated at 12 000 rpm for 10 min to remove Mn‐N/C material and cellular debris. Subsequently, the supernatant was acquired and underwent assessment for manganese elemental content using an Agilent 7700 Inductively Coupled Plasma Mass Spectrometer.
3
Elemental and Spectroscopic Analysis
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Elemental analysis was performed by standard microanalysis on an Elementar Analysensysteme GmbH: vario MICRO cube analyzer and ICP + MS was done by means of an Agilent 7700 inductively coupled plasma mass spectrometer (ICP + MS). Infrared absorption spectra were recorded with a JASCO IRT-3100 FT-IR microscope.
4
Isolation of Cd-Resistant Bacillus sp. from Contaminated Soil
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Cd-contaminated soil was collected from a former industrial site in Hunan Province (27°46′N, 112°52′E) and analyzed for Cd content through acid digestion followed by the use of a 7700 × inductively coupled plasma mass spectrometer (Agilent Technologies, Tokyo, Japan). To isolate Cd-resistant and bio-safe bacteria, aerobic Bacillus sp. were isolated from the soil by plating on LB agar plates containing progressively higher concentrations of cadmium chloride (0, 0.5, 1 and 2 mM). Then, in order to isolate aerobic Bacillus sp., the bacterial enrichment cultures were heat-shocked at 80 °C for 20 min. Bacterial genomic DNA was isolated using the TIANamp Bacteria DNA kit (TIANGEN Biotech). The 16S rRNA gene was amplified from the extracted DNA using the universal primers 16S rRNA-F/16S rRNA-R (Additional file 2 : Table S2) and the amplification products were cloned in the pGM-T (TIANGEN Biotech) vector using competent E. coli TOP10 cells (TIANGEN Biotech). Sequencing was carried out using T7 and SP6 primers and compared to the GenBank database using the NCBI BLAST program.
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