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5 protocols using lc ms grade ultra pure water

1

Metabolic Profiling of Microbial Samples

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D-glucose, L-glutamic acid, NH4Cl, K2HPO4, MgSO4·7 H2O, CaCl2·2 H2O, MnSO4·H2O, FeCl3·6 H2O, ZnSO4·7 H2O, Na2-EDTA, CuSO4·5 H2O, and CoCl2·6 H2O were purchased from Carl Roth GmbH+Co. KG (Karlsruhe, Germany). LC/MS-grade ultra-pure water, HPLC-grade chloroform, acetic acid, H2SO4, and NH4HCO3 were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). 10-Camphorsulfonic acid and tributylamine were purchased from SigmaAldrich (MO, USA).
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2

Analytical Standards for Pharmaceutical Compounds

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Standards of imipramine hydrochloride (Lot no: SKL0836, molecular weight: 316.87 g/mole) and chloroquine diphosphate (Lot no: LEE6907, molecular weight: 515.86 g/mole) were purchased from Fujifilm Wako Pure Chemical Industries (Osaka, Japan). Hematoxylin, eosin, xylene, pathomount, LC-MS grade ultrapure water, and ethanol were also purchased from Fujifilm Wako Pure Chemical Industries (Osaka, Japan). Super cryo-embedding medium (SCEM) and optimum cutting temperature (OCT) compounds were purchased from Waters, Section Lab, and Sakura Finetek Japan, respectively. Matrix α-cyano-4-hydroxycinnamic acid (CHCA) was purchased from Sigma-Aldrich (St. Louis, MO, USA).
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3

Extraction and LC/MS Analysis of NAD

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NAD was obtained from Nacalai Tesque (Japan). NGD and NHD were purchased from Sigma-Aldrich (USA). LC/MS grade ultrapure water and methanol were obtained from Wako Pure Chemical Industries, Ltd. (Japan).
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4

Metabolite Extraction and Analysis

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D-Glucose, L-glutamic acid, NH4Cl, K2HPO4, MgSO4 × 7H2O, CaCl2 × 2H2O, MnSO4 × H2O, FeCl3 × 6H2O, ZnSO4 × 7H2O, Na2-EDTA, CuSO4 × 5H2O and CoCl2 × 6H2O were purchased from Carl Roth GmbH + Co., KG (Karlsruhe, Germany). LC/MS-grade ultra-pure water, HPLC-grade chloroform, acetic acid, H2SO4 and NH4HCO3 were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). 10-Camphorsulfonic acid and tributylamine were purchased from SigmaAldrich (St. Louis, MO, United States).
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5

Hesperidin Quantification by LC/MS

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LC/MS-grade ultrapure water, HPLC-grade methanol, and dimethyl sulfoxide were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). LC/MS-grade acetonitrile and formic acid (eluent additives for LC/MS) were purchased from Honeywell Fluka Chemicals (Morristown, NJ). Hesperidin standard for analysis and chromatography grade was obtained from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan).
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