For FACS analysis, the neurospheres were dissociated into single cell by accutase digestion. Single cell suspensions were then stained with appropriate antibodies as follows: mouse anti-nestin (1 : 50, Abcam), rat anti-CXCR4 (1 : 50, BioLegend), and rabbit anti-βIII tubulin (1 : 10, Epitomics). All flow cytometric analysis was performed using FACSCAria II (BD Biosciences) and results were analyzed by FACSDiva software (BD Biosciences).
Facscaria 2
Manufactured by BD
The FACSCAria II is a high-performance cell sorter designed for advanced flow cytometry applications. It features a compact and modular design, providing users with flexibility in configuration and capability. The core function of the FACSCAria II is to enable the efficient sorting and analysis of complex cell samples.
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Lab products found in correlation
2 protocols using facscaria 2
1
Neural Stem Cell FACS Analysis
2
Cell Cycle Analysis of mNPCs
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To determine the distribution of mNPCs within G1, S, and G2/M phases of the cell cycle, a flow cytometry cell cycle analysis kit was used (Abcam) and the manufacturer's protocol was followed. Briefly, the neurospheres were dissociated into a single cell by accutase digestion and fixed in 75% ice-cold ethanol overnight at 4°C. The fixed cells were then stained with 50 μg/ml propidium iodide (PI) containing RNase A for 30 min at room temperature. Cell fluorescence was assessed using FACSCAria II (BD Biosciences), and results were analyzed by FACSDiva software (BD Biosciences).
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