P. aeruginosa PAO1 strain (from ATCC 15692, Manassas, USA), was used to prepare bacteria-loaded agar beads. Ciprofloxacin base (CIP) powder (purity ≥98,0%), copper hydroxide Cu(OH)2, hyaluronic acid (HA) sodium salt from Streptococcus equi, calcium hydroxide Ca(OH)2, formic acid and ammonium carbonate (NH4)2CO3 were purchased from Sigma Aldrich (Saint-Quentin Fallavier, France).
Ammonium carbonate nh4 2co3
Manufactured by Merck Group
Sourced in France, Czechia, Germany, Italy, Ireland
Ammonium carbonate (NH4)2CO3 is a chemical compound commonly used in laboratory settings. It is a white, crystalline solid that is soluble in water. Ammonium carbonate has a variety of applications in the scientific and industrial fields.
Automatically generated - may contain errors
Lab products found in correlation
Calcium hydroxide ca oh 2, by Merck Group (2 mentions)
Hyaluronic acid sodium salt from streptococcus equi, by Merck Group (2 mentions)
Formic acid, by Merck Group (1 mentions)
Tris hydroxymethyl aminomethane, by Merck Group (1 mentions)
Rosmarinic acid, by Biosynth (1 mentions)
Deuterium oxide, by Eurisotop (1 mentions)
Sodium deuteroxide, by Eurisotop (1 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (1 mentions)
Milli q, by Merck Group (1 mentions)
Lc ms grade acetonitrile, by Merck Group (1 mentions)
Bondesil, by Agilent Technologies (1 mentions)
Hplc grade acetonitrile, by Merck Group (1 mentions)
Hanks balanced salt solution (hbss), by Thermo Fisher Scientific (1 mentions)
6 protocols using ammonium carbonate nh4 2co3
1
Pseudomonas aeruginosa Infection Model
2
Calcium Chloride and Ammonium Carbonate Crystallization
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For crystallization experiments, anhydrous calcium chloride (CaCl2) was purchased from Thermo Fisher Scientific, and ammonium carbonate [(NH4)2CO3] was purchased from Sigma-Aldrich. Bovine phosphorylated milk OPN (with approximately 24 phosphorylations per molecule) was provided by Arla Foods and was prepared according to the method described by Sørensen and Petersen (53 (link)).
3
Synthesis of Silver-Zinc Polymer Composite
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Silver nitrate AgNO3 (>99.5% purity) and Zinc nitrate hexahydrate (ZnNO3)2·6H2O (>99% purity) were delivered by Penta (Praha, Czech Republic), while ammonium carbonate (NH4)2·CO3 (>99% purity) was supplied by Sigma-Aldrich (Praha, Czech Republic). Demineralized water was used throughout the experiments. Thermoplastic polyolefin elastomer of type Versify 3401 (Dow Europe GmbH; Rheinmünster, Germany) and polypropylene random copolymer TOTAL PPR 6298 S01 (Total Petrochemicals & Refining S.A./N.V., Bruxelles, Belgium) were utilized as the polymer matrix or blends, respectively.
4
Antioxidant Compound Quantification
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Chlorogenic acid (CA, min 98%) and rosmarinic acid (RA, min 98%) were purchased from Carbosynth Limited, Compton, United Kingdom. Calcium chloride dihydrate (>99%) was obtained from Jessen Chemicals, Hamburg, Germany. Tris(hydroxymethyl)aminomethane (TRIS, >99.8%), 2,2-diphenyl-1-picrylhydrazyl (DPPH, 97%) and ammonium carbonate ((NH4)2CO3, 99.99%) were purchased from Sigma-Aldrich, Darmstadt, Germany. Deuterium oxide (D2O, D 99.9%) and sodium deuteroxide (NaOD, D 99.5%, 40% in D2O) were obtained from Eurisotop, Saclay, France.
5
Ergot Alkaloid Quantification Protocol
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Chemicals and solvents used for sample preparation were ‘pro-analysis’ quality or better. Milli-Q (Merk Millipore S.p.a, Milano, Italy) water was used. LC-MS grade acetonitrile (ACN) and ammonium carbonate (NH4)2CO3 were purchased from Sigma-Aldrich (Milano, Italy). Bondesil (Agilent Technologies, Santa Clara, CA, USA) Primary Secondary Amine (PSA) was used.
The analytical reference standards of ergometrine, ergosine, ergocornine, α-ergocryptine, ergotamine, ergocristine, ergometrinine, ergosinine, ergocorninine, α-ergocryptinine, ergotaminine, ergocristinine were purchased as dry film from Biopure (Tulln, Austria).
A standard mix solution was prepared in 100% ACN as a mix of all the EAs under examination at a concentration of 0.5 μg/mL of each of the alkaloids. This solution was diluted in ACN at 10 ng/mL to obtain a standard working solution used for preparation of calibration curves. All standard solutions were stored at −20 °C in amber vials.
The analytical reference standards of ergometrine, ergosine, ergocornine, α-ergocryptine, ergotamine, ergocristine, ergometrinine, ergosinine, ergocorninine, α-ergocryptinine, ergotaminine, ergocristinine were purchased as dry film from Biopure (Tulln, Austria).
A standard mix solution was prepared in 100% ACN as a mix of all the EAs under examination at a concentration of 0.5 μg/mL of each of the alkaloids. This solution was diluted in ACN at 10 ng/mL to obtain a standard working solution used for preparation of calibration curves. All standard solutions were stored at −20 °C in amber vials.
6
Ciprofloxacin-Hyaluronic Acid Formulation
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Ciprofloxacin (CIP) powder (purity ≥ 98,0 %), high molecular weight (1.5 to 1.8 x 10 6 Da), hyaluronic acid sodium salt from Streptococcus equi (HA), calcium hydroxide Ca(OH) 2 and ammonium carbonate (NH 4 ) 2 CO 3 were purchased from Sigma Aldrich (Ireland). Buffer concentrate pH 5 (Titrisol) was purchased from Merck (Germany) and Hank's Balanced Salt Solution (HBSS) was purchased from Gibco (France). Deionised water was produced by a Purite Prestige Analyst HP water purification system (Millipore, Carrigtwohill, Ireland). HPLC grade acetonitrile was purchased from Sigma-Aldrich (Ireland).
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