Cancer cells were seeded onto sterile slides into 24-well culture plates. Then, fixed cells with 4% paraformaldehyde for 20 min when reached a confluence of 80%. Following permeabilized membranes with 0.1% Triton X-100 for 10 min, blocked antigens with 10% goat serum for 30 min in room temperature. Cells were then incubated with anti-hnRNPA2B1 (ab6102, Abcam) at a 1:100 dilution overnight at 4 °C, followed by further incubation at room temperature for 1 h with AlexaFluor Plus 555 goat anti-mouse IgG secondary antibody (A32727, Thermo) and then labeled DNA with DAPI for 10 min.
Alexafluor plus 555 goat anti mouse igg secondary antibody
Manufactured by Thermo Fisher Scientific
The AlexaFluor Plus 555 goat anti-mouse IgG secondary antibody is a fluorescently-labeled antibody that binds to mouse immunoglobulin G (IgG) molecules. It is designed for use in immunofluorescence and other fluorescence-based applications.
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2 protocols using alexafluor plus 555 goat anti mouse igg secondary antibody
1
Immunofluorescence Assay for hnRNPA2B1
2
Immunofluorescent Localization of hnRNPA2B1
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Cancer cells were seeded onto sterile slides into 24-well culture plates. Then, xed cells with 4% paraformaldehyde for 20 min when reached a con uence of 80%. Following permeabilized membranes with 0.1% Triton X-100 for 10 min, blocked antigens with 10% goat serum for 30 min in room temperature.
Cells were then incubated with anti-hnRNPA2B1 (ab6102, Abcam) at a 1:100 dilution overnight at 4 °C, followed by further incubation at room temperature for 1 h with AlexaFluor Plus 555 goat anti-mouse IgG secondary antibody (A32727, Thermo) and then labeled DNA with DAPI for 10 min.
Cells were then incubated with anti-hnRNPA2B1 (ab6102, Abcam) at a 1:100 dilution overnight at 4 °C, followed by further incubation at room temperature for 1 h with AlexaFluor Plus 555 goat anti-mouse IgG secondary antibody (A32727, Thermo) and then labeled DNA with DAPI for 10 min.
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