All chemicals and reagents purchased from sigma Aldrich and Daejung chemicals South Korea, and TCI chemicals, Japan without further purifications. Absorption spectra and drug release study were recorded in 1 cm quartz cuvettes using Evolution™ 60 UV–visible Spectrophotometer (Thermo Fischer Scientific, USA). Emission spectra were obtained on a Jasco-FP 6500 spectrofluorometer. Particle size was analyzed by Dynamic Light Scattering Spectrophotometer (Otsuka Electronics Co., Ltd, Japan). Zeta potential was measured by electrophoretic light scattering (ELS) spectrometer (Otsuka Electronics Co., Ltd, Japan). The morphology of the Ru-1@TPP-PEG-Biotin SAN was analyzed using Energy-Filtering Transmission Electron Microscope (EF-TEM, Carl Zeiss, LIBRA 120, Germany). Fourier transform infrared (FTIR) spectroscopy experiments performed using a JASCO, FT/IR-4200 instrument. The human breast cancer cell line MCF-7 and hepatoma cell line HepG2 were supplied from the Korean Cell Line Bank (KCLB, Korea). Quantum dot conjugation kit (Invitrogen, USA) was used to conjugate the antibody with a quantum dot.
Ft ir 4200 instrument
Manufactured by Jasco
The FT/IR-4200 is a Fourier Transform Infrared (FT-IR) spectrometer. It is designed to perform infrared spectroscopy, a technique used to identify and analyze the chemical composition of various substances. The instrument measures the absorption of infrared radiation by the sample, providing information about the molecular structure and functional groups present.
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3 protocols using ft ir 4200 instrument
1
Synthesis and Characterization of Ru-1@TPP-PEG-Biotin SAN
2
Characterization of Ru-1@TPP-PEG-biotin Self-Assembled Nanoparticles
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The zeta potential and size of the particle were analyzed using dynamic light scattering and electrophoretic light scattering spectrometers. The in vitro size and charge stability SANs was performed by mixing the nanoparticle with 3 ml of 1 × PBS (pH 7.4) and incubated at 37 °C. Then, the nanoparticle solution was withdrawn at different time intervals (0, 1, 3, and 5 days) and analyzed using DLS and ELS. The morphology of the Ru-1@TPP-PEG-biotin SAN was analyzed using Energy-Filtering Transmission Electron Microscope. Briefly, 8 µl of the self-assembled nanoparticle solution in PBS was dropped on the copper grid and dried for 2 days. The size and shape of the SANs were then analyzed using TEM. The Ru-1 loaded TPP-PEG-biotin SAN was also confirmed by Fourier transform infrared (FTIR) spectroscopy using a JASCO, FT/IR-4200 instrument at room temperature. Spectra were recorded in the range of 4000–600 cm−1.
3
Structural Analysis of Exopolysaccharides
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With the aim of elucidating the type of EPS isolated from the two strains neutral sugar composition and linkage types were determined as previously described (Notararigo et al., 2013) (link). Neutral sugars were identified and quantified by gas chromatography, after hydrolysis of polysaccharides' samples with 3 M trifluoroacetic acid (TFA) for 90 min and derivatization to alditol acetates. To determine linkage types, the polysaccharides were methylated according to Ciucanu and Kerek (Ciucanu & Kerek, 1984) , hydrolyzed with TFA 3 M for 1 h at 120 °C, converted into partially methylated alditol acetates using sodium borodeuteride as the reducing agent and analyzed by gas-chromatography/mass spectrometry. The linkages in the polysaccharides were deduced from the mass spectra and retention time of the peaks, and their relative amount from the area under each peak. For Fourier-Transformed Infrared Spectroscopy (FTIR) analysis, KBr pellets of the samples were first prepared, recording the spectra in a FTIR 4200 instrument (Jasco Corporation) in the range 4000-700 cm -1 . The number of scans per experiment was 50, with a resolution of 4 cm -1 .
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