Omniscript reverse transkriptase kit

Complete RNA was isolated from native lung tissue before transplantation from both, donor and recipient, with NucleoSpin RNA Kit (Macherey & Nagel, Dueren, Germany). Subsequent cDNA synthesis was accomplished with Omniscript Reverse Transkriptase-Kit (QIAGEN, Hilden, Germany) with oligo-dT Primers. cDNA was amplified with HotStar Taq polymerase (QIAGEN) using oligonucleotide primers ScDQB5a: 5′-GGC TGT GTT GAC TAC CAT TA-3′ and ScDQB3a: 5′-AGA CCA GCA GGT TGT GG-3′ for the second exon (β1 domain) of the MHC II molecule.^{12 (link)} Clean up of DNA was facilitated by NucleoSpin Extract II (Macherey & Nagel, Dueren, Germany). Sequencing of both strands was done by SeqLab (Goettingen, Germany).