Bovine hemoglobin and GSH were purchased from Aladdin (Shanghai, China); transferrin and doxorubicin hydrochloride salt were supplied by MACKLIN (Shanghai, China); PpIX was bought from Energy Chemical (Shanghai, China); ROS detection Kit was obtained from Beyotime (Jiangsu, China); TFRs was purchased from Mei Mian Biotechnology Co., Ltd. (Jiangsu, China); Mouse ALT Kit, BUN Kit, CRE Kit, AST Kit were obtained from Nanjing Jiancheng Bioengineering Institute (Jiangsu, China); Hemoglobin was supported by ToYongBio Tech Inc. (Shanghai, China); Monolith RED‐NHS protein labeling kit were purchased from NanoTemper Technologies GmbH; Hypoxyprobe‐1 plus kit was purchased from Hypoxyprobe Inc.; JC‐1 assay kit, apoptosis assays kit, and Calcein/ PI cell viability assay kit were obtained from keygen biotech (Jiangsu, China).
Bun kit
Manufactured by Nanjing Jiancheng
Sourced in China
The BUN kit is a laboratory equipment used for the quantitative determination of urea nitrogen in biological samples. It provides a precise and reliable method to measure blood urea nitrogen (BUN) levels.
Automatically generated - may contain errors
Lab products found in correlation
Creatinine assay kit, by Nanjing Jiancheng (4 mentions)
Monolith protein labeling kit red nhs, by NanoTemper (1 mentions)
Ast kit, by Nanjing Jiancheng (1 mentions)
Apoptosis assays kit, by Keygen Biotech (1 mentions)
Ros detection kit, by Beyotime (1 mentions)
1 plus kit, by Hypoxyprobe (1 mentions)
Jc 1 assay kit, by Keygen Biotech (1 mentions)
Urinary albumin assay kit, by Nanjing Jiancheng (1 mentions)
Tg kit, by Nanjing Jiancheng (1 mentions)
Appropriate secondary antibody, by Santa Cruz Biotechnology (1 mentions)
Anti nphs2, by Abcam (1 mentions)
Anti gapdh primary antibody, by Santa Cruz Biotechnology (1 mentions)
Anti caspase 3, by Abcam (1 mentions)
Anti bcl 2, by Abcam (1 mentions)
Tunel kit, by Roche (1 mentions)
Anti bax, by Abcam (1 mentions)
Anti tgf β1, by Abcam (1 mentions)
Ua kit, by Nanjing Jiancheng (1 mentions)
Creatinine kit, by Nanjing Jiancheng (1 mentions)
11 protocols using bun kit
1
Bovine Hemoglobin-based Nanoparticles for Cancer Therapy
2
Creatinine and BUN Detection
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Scr was detected by a Creatinine Assay Kit and BUN was detected by a BUN kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China), according to the manufacturer’s instructions.
3
Biomarker Measurement in Metabolic Study
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BGL was measured using a hand-held glucometer (Shanghai Qiangsheng Medical Equipment, Shanghai, China). Urinary albumin concentration was identified using the urinary albumin assay kit (Jiancheng Biotechnology, Nanjing, China). Triglycerides (TG) and total cholesterol (TC) were determined using a TG kit and a cholesterol kit (Jiancheng Biotechnology, Nanjing, China), respectively. The renal function was assessed by measuring serum creatinine (Scr) and blood urea nitrogen (BUN). Both the Scr colorimetric determination kit and the BUN kit were purchased from Jiancheng Biotechnology, Nanjing, China. All procedures were carried out in accordance with manufacturer’s instructions.
4
Serum Creatinine and BUN Analysis
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The levels of serum creatinine (SCr) and blood urea nitrogen (BUN) were determined by utilizing a Creatinine Assay Kit and a BUN kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China), respectively, in accordance with the instructions provided by the manufacturer.
5
Assessing Renal Function: Serum Creatinine and BUN
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Renal functions were assessed by measuring serum creatinine and blood urea nitrogen (BUN) levels. Briefly, the blood samples were initially kept at room temperature for about 20 minutes, following which they were centrifuged to collect the clear serum. Serum creatinine and BUN levels were evaluated to determine renal functions with serum creatinine kit (Jiancheng, China, catalog C011-2-1) and BUN kit (Jiancheng, China, catalog C013-2-1). For serum creatinine measurement, standards, serum samples and blank water were alternatively mixed with 180μl Enzyme Solution A in the 96-well plate, which was incubated in a 37°C incubator for five minutes. After that OD546nm readings were retrieved. 60μl Enzyme Solution B was added, and the plate was maintained at 37°C for another 5 minutes. The second OD546nm readings were retrieved. The concentration of serum creatinine levels was calculated accordingly. For serum BUN levels, 20μl serum samples, standards or blank water were alternatively mixed with 250μl urease solution. The mixtures were maintained for 10min at 37°C. Phenol solution (1ml) and sodium hypochlorite solution (1ml) were then added, and the tubes containing the mixtures were immersed in 37°C water bath for 10 minutes. Finally, OD640 readings were retrieved, which were used for the calculation of BUN levels.
6
Serum Creatinine and BUN Analysis
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Blood samples were collected from mice eyes. The serum was made by centrifugation at speed of 1500rpm for 15 min at 4 °C, and the serum Scr level was determined by Creatinine Assay Kit; the serum BUN level was detected by a BUN kit (Nanjing Jiancheng Bioengineering Institute, Nanjing, China), in accordance with the manufacturer's instructions.
7
Renal Protection by Microbubble-Mediated Drug Delivery
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Egg Yolk Lecithin (PC, PC-98T) and cholesterol (98% of purity) were purchased from Shanghai Advanced Vehicle Technology Pharmaceutical L.T.D. Co (AVT). Anti-Bax, anti-Bcl-2, anti-NPHS2, anti-caspase-3 and anti-TGFβ1 antibodies were obtained from Abcam (Abcam, Cambridge, MA). Anti-GAPDH primary antibody, and appropriate secondary antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). CRE, TG and BUN kit were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing Jiancheng Bioengineering Institute, Nanjing, China). TUNEL kit was obtained from Roche (Roche, Shanghai, China). All other reagents were obtained from Sigma-Aldrich (Sigma-Aldrich, St. Louis, MO). Simulated body fluid (SBF) were purchased from Beijing leagene Biotechnology Company (Beijing leagene Biotechnology Company, Beijing, China). MB was prepared by the sonication-lyophilization method as reported in our recent study [46 (link)].
8
Measuring Urea and Albumin Secretion
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During cell culture, the supernatant of the medium was collected every two days. The urea concentration in the supernatant was measured using a BUN kit (Nanjing Jiancheng Bioengineering Institute, China), and albumin secreted by hiHeps was measured with a human albumin enzyme‐linked immunosorbent assay kit (Elisa, Bethyl, USA) according to the manufacturer's instructions.
9
Serum Renal Biomarkers Analysis
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The blood samples were collected from the eyeballs of the mice, and the serum was separated via centrifugation at 4000× g for 30 min. The serum samples were deposited into clean 200 μL EP tubes, and the levels of the renal function indicators blood urea nitrogen (BUN), creatinine (Cr), and uric acid (UA) were determined using the BUN kit, Cr kit, and UA kit (Nanjing Jiancheng bioengineering Institution, Nanjing, China), respectively, according to the manufacturers’ instructions.
10
Comprehensive Renal Assessment Protocol
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The levels of blood urea nitrogen (BUN), serum and urine creatinine (Scr, Cre), urinary protein (UPr), TNFα, IL1β, IL-5 and IL-6 in serum and urine of rats were determined by ELISA. All steps were strict to the instructions. After completing the last step, the absorbance at the specified wavelength was detected using a multifunctional enzyme-labeled analyzer (MB-530, Heales) within 5 min. The serum and urinary creatinine were measured by the creatinine kit (#C011-2-1, Jiancheng) and the Creatinine clearance rate (Ccr= Cre × urine volume per minute/Scr) was calculated accordingly. The BUN kit (#C013-1-1), and UPr kit (#C035-2-1) were purchased from Nanjing Jiancheng Bioengineering Institute. Sino-American Biotechnology Co., Ltd provided the TNFα kit (#CSB-E11987r), IL1β kit (#CSB-E08055r), IL-5 kit (#CSB-E07435r), and IL-6 kit (#CSB-E04640r).
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