Anti rabbit igg hrp sc 2004

Dulbecco's modified Eagle's medium (DMEM) and penicillin-streptomycin solution used in cell cultures were purchased from Hyclone (GE Healthcare Life Sciences, Logan, UT, USA). Newborn calf serum was purchased from Gibco (Thermo Fisher Scientific, Inc., Waltham, MA, USA). Nuclear and Cytoplasmic Protein Extraction kit (163–2089) was purchased from Bio-Rad Laboratories Inc., (Hercules, CA, USA). Mouse anti-human monoclonal E6 antibody (ab51931) was purchased from Abcam (Cambridge, UK). In addition, anti-mouse FITC secondary antibody (200-032-037) was purchased from Jackson Immunoresearch Inc., (West Grove, PA, USA), while the p53 anti-mouse (BA0521) and anti-mouse β-actin (BA2305) antibodies were purchased from Boster Biological Technology, Ltd., (Wuhan, China). CDV, Goat anti-mouse IgG-HRP (sc-2005) and anti-rabbit IgG-HRP (sc-2004) were purchased from Santa Cruz Biotechnology Inc., (Shanghai, China). Alexa Fluor 594-conjugated donkey anti-rabbit IgG secondary antibody (R37119) for immunofluorescence staining was purcahsed from Thermo Fisher Scientific, Inc. DDP and dimethyl sulfoxide (DMSO) reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA), all of which were dissolved in 0.01 M phosphate-buffered saline (PBS) to make a 50 µM stock solution, and diluted with DMEM to the required concentration prior to use.