Log In Sign up
The largest database of trusted experimental protocols

Nucleospin tissue dna extraction kit

Manufactured by Macherey-Nagel
Visit Supplier
Sourced in Germany, France

The NucleoSpin Tissue DNA extraction kit is a laboratory product designed for the isolation and purification of genomic DNA from various tissue samples. It utilizes a silica-membrane technology to efficiently capture and elute DNA, providing a reliable and consistent method for DNA extraction.

Automatically generated - may contain errors

Lab products found in correlation

Nanodrop one, by Thermo Fisher Scientific (4 mentions) Precellys 24 dual, by Bertin Technologies (3 mentions) Qubit 2.0 fluorometer, by Thermo Fisher Scientific (3 mentions) Qubit 3.0 fluorometer, by Thermo Fisher Scientific (3 mentions) Nextera xt library preparation kit, by Illumina (2 mentions) Nextseq, by Illumina (2 mentions) Ficoll paque plus, by GE Healthcare (2 mentions) Precellys 24 dual homogenizer, by Bertin Technologies (2 mentions) Nanodrop spectrophotometer, by Thermo Fisher Scientific (2 mentions) Nucleospin soil dna extraction kit, by Macherey-Nagel (2 mentions) Firepol master mix, by Solis BioDyne (1 mentions) Nucleospin gel and pcr clean up kit, by Macherey-Nagel (1 mentions) Platinum taq polymerase, by Thermo Fisher Scientific (1 mentions) Miseq platform, by Illumina (1 mentions) Magnetic bead selection, by Miltenyi Biotec (1 mentions) Polya spin mrna isolation kit, by New England Biolabs (1 mentions) Truseq small rna library construction kit, by Illumina (1 mentions) Superscript 3, by Thermo Fisher Scientific (1 mentions) Hiseq 2500, by Illumina (1 mentions) Thermocycler, by Bioer (1 mentions)

41 protocols using nucleospin tissue dna extraction kit

1

ExPEC and UPEC Genotyping in E. coli

Check if the same lab product or an alternative is used in the 5 most similar protocols
Phylogroups and ExPEC VG carriage of the 104 E. coli were identified using PCR-based methods. Genomic DNA was extracted using Nucleospin® Tissue DNA extraction kits (Machery-Nagel, Germany). Clermont E. coli phylo-typing, which consists of a quadruplex PCR panel and two simplex PCRs, was performed to differentiate E. coli phylogroup A, B1, B2, C, D, E, F and E. coli cryptic clades35 (link). VGs associated with extraintestinal pathogenesis of E. coli, including adhesion (afa, crl, fimA, iha, papC, sfa/foc, yfcV, and tsh), cytotoxicity (hlyA, hlyE, cnf, sat, and vat), iron acquisition (iroN, fyuA, irp1, chuA, iutA, and iucD), and bacterial cell protection (bssS, hmsP, iss, and kpsII) were detected by PCR (see Supplementary Table S4 online). All PCRs were conducted in a 25 µL total volume containing 5X Firepol Master Mix (Solis Biodyne, Estonia), and the concentration of each primer was 0.2 µM.
Isolates that tested positive for ≥ 2 out of 5 VGs (papC, sfa/foc, afa, iutA, and kpsII) were presumably classified as ExPEC, whereas isolates demonstrating ≥ 3 out of 4 VGs (yfcV, vat, fyuA, and chuA) were presumably classified as UPEC4 (link),6 (link).
Sroithongkham P., Nittayasut N., Yindee J., Nimsamer P., Payungporn S., Pinpimai K., Ponglowhapan S, & Chanchaithong P. (2024). Multidrug-resistant Escherichia coli causing canine pyometra and urinary tract infections are genetically related but distinct from those causing prostatic abscesses. Scientific Reports, 14, 11848.
+ Open protocol
+ Expand
2

Antimicrobial Resistance in Feline and Canine Staphylococcus aureus

Check if the same lab product or an alternative is used in the 5 most similar protocols
Staphylococcus aureus isolates were obtained from clinical samples, from diseased cats and dogs submitted to the Veterinary Diagnosis Laboratory of Chulalongkorn University, Bangkok, Thailand, from January 2017 to June 2020. The species identification and antimicrobial susceptibility testing was routinely performed via the Vitek® automated system, with ID-GP and AST-GP81 cards (bioMérieux, France) for clinical purposes. Bacterial strains were stocked in an −80 °C freezer with 20% glycerol in tryptic soy broth. The S. aureus isolates presenting cefoxitin resistance by Vitek® were included for species confirmation, by Microflex Biotyper matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) (Bruker Dalnotics, Germany) and nuc gene PCR [45 (link)], after recovery onto 5% sheep blood agar and DNA extraction using Nucleospin Tissue DNA extraction kits (Machery-Nagel, Germany). The DNA was also used in all PCR-based molecular assays.
Chueahiran S., Yindee J., Boonkham P., Suanpairintr N, & Chanchaithong P. (2021). Methicillin-Resistant Staphylococcus aureus Clonal Complex 398 as a Major MRSA Lineage in Dogs and Cats in Thailand. Antibiotics, 10(3), 243.
+ Open protocol
+ Expand
3

Molecular Identification of Marteilia

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
For the molecular identification of the Marteilia protozoan pathogen, a small piece of approximately 25 mg of the homogenized digestive gland and mantle tissue was subjected to DNA extraction, using the Nucleospin Tissue DNA extraction kit (Macherey Nagel, Düren, Germany), following the manufacturer’s guidelines. The primer pair SS2/SAS2 was used in a 10 μL volume PCR for each individual, containing 1 μL extracted DNA of approximately 50 ng/μL concentration, 0.3 pmol of each primer, 5 μL FastGene® Taq ReadyMix (2×) and water up to the final volume of 10 μL. Conditions were 95 °C for 3 min and then 94 °C for 30 s, 52 °C for 40 s and 72 °C for 45 s, repeated 36 times and followed by an extension step of 5 min at 72 °C. PCR products were visualized in an agarose gel after electrophoresis stained with ethidium bromide. A previously identified Marteilis refrigens sample [53 (link)] was utilized in each reaction as a positive control. Positive samples were cleaned using the NucleoSpin Gel and PCR Clean-up kit (Macherey Nagel, Düren, Germany) and bidirectionally sequenced for identification of the species.
Lattos A., Papadopoulos D.K., Feidantsis K., Karagiannis D., Giantsis I.A, & Michaelidis B. (2022). Are Marine Heatwaves Responsible for Mortalities of Farmed Mytilus galloprovincialis? A Pathophysiological Analysis of Marteilia Infected Mussels from Thermaikos Gulf, Greece. Animals : an Open Access Journal from MDPI, 12(20), 2805.
+ Open protocol
+ Expand
4

Alfalfa WD40-1 Promoter Sequencing

Check if the same lab product or an alternative is used in the 5 most similar protocols
Due to lack of alfalfa genome sequence, we used Clonetech GenomeWalker™ (California, USA Cat No. 638904) to obtain nucleotide sequence of the WD40–1 promoter region. In brief, we extracted genomic DNA from wild-type alfalfa plants using a Nucleospin®Tissue DNA extraction kit (MACHEREY-NAGEL Gmbh & Co. KG Germany, Cat. No. 740952). GenomeWalker “libraries” were prepared by digesting the DNA with four different restriction enzymes (DraI, EcoRV, PvuII and StuI) at 37 °C for 2 h to generate blunt ends. Subsequently, two nested PCR amplifications were performed sequentially for each library using gene specific primers (GSP1 and GSP2) and adapter primers (AP1 and AP2) from the kit (Additional file 1: Table S1). PCR products were analyzed on a 1.5% agarose gel followed by cloning into a pJET1.2 cloning vector to facilitate sequencing. Subsequently, sequences obtained from the four libraries were aligned together to generate the consensus promoter region sequence of WD40–1 in alfalfa.
Feyissa B.A., Arshad M., Gruber M.Y., Kohalmi S.E, & Hannoufa A. (2019). The interplay between miR156/SPL13 and DFR/WD40–1 regulate drought tolerance in alfalfa. BMC Plant Biology, 19, 434.
+ Open protocol
+ Expand
5

Molecular Detection of Antimicrobial Resistance

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Genomic DNA was isolated using the Nucleospin Tissue DNA extraction kit (Machery Nagel,
Düren, Germany). Acquired AMR genes, which encode mechanisms of resistance to carbapenems
(blaOXA-23-like), tetracyclines [tet(B) and
tet(39)] and aminoglycosides (strA, strB, aac(3)-Iaand aac(6´)-Im) in Acinetobacter spp., were detected by
simplex PCRs (Supplementary Table 1). Additional aminoglycoside-modifying enzyme
(AME)-encoding genes including aac(3)-IIa, aac(6´)-Ih,
aph(3′)-VI, ant(2´´)-Ia, aph(3′)-Ia and
aac(6´)-Ib were identified by multiplex PCRs [30 (link)]. A 25-μL PCR mixture was prepared using the 5X Firepol Master Mix
(Solis BioDye, Tartu, Estonia) with 0.2 μM of each primer. Entire
blaOXA-23 were analyzed by Sanger’s capillary sequencing.
Specific regions of ISAba1 and ISAba4 at both
extremities of the blaOXA-23-containing transposon were
amplified by PCR to illustrate the presence of transposable elements [25 (link)].
LEELAPSAWAS C., YINDEE J., NITTAYASUT N., CHUEAHIRAN S., BOONKHAM P., SUANPAIRINTR N, & CHANCHAITHONG P. (2022). Emergence and multi-lineages of carbapenemase-producing Acinetobacter baumannii-calcoaceticus complex from canine and feline origins. The Journal of Veterinary Medical Science, 84(10), 1377-1384.
+ Open protocol
+ Expand
6

Genomic DNA Extraction from Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tissue samples were individually crushed with glass beads using a Precellys®24 Dual homogeniser (Bertin technologies, Paris, France) at 5500× rpm for 20 s. Genomic DNA was then extracted using a Nucleospin tissue DNA extraction Kit (Macherey-Nagel, Hoerdt, France). For each sample, total DNA was eluted in 50 µl of rehydration solution and stored at -20 °C until further analysis.
Lejal E., Moutailler S., Šimo L., Vayssier-Taussat M, & Pollet T. (2019). Tick-borne pathogen detection in midgut and salivary glands of adult Ixodes ricinus. Parasites & Vectors, 12, 152.
+ Open protocol
+ Expand
7

DNA Extraction from T-Cell Suspensions

Check if the same lab product or an alternative is used in the 5 most similar protocols
T1-cell suspensions (180 μL) were treated with proteinase K (25 µL) for 2 h at 56 °C. Afterwards, the samples went through a DNA extraction protocol using the NucleoSpin® Tissue DNA extraction kit (Macherey-Nagel, Germany), following the manufacturer’s recommendations. DNA was stored at −20 °C until use.
Reynaud Y., Ducat C., Talarmin A, & Marcelino I. (2020). Cartography of Free-Living Amoebae in Soil in Guadeloupe (French West Indies) Using DNA Metabarcoding. Pathogens, 9(6), 440.
+ Open protocol
+ Expand
8

Tick Homogenization and DNA Extraction

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
In total, 998 nymphs have been collected over the 3 years [26 (link)]. As detailed in our previous studies [26 (link), 29 (link)], ticks were first washed once in ethanol 70% for 5 min and rinsed twice in sterile MilliQ water. They were then individually homogenised in 375 μL of Dulbecco’s modified Eagle’s medium with decomplemented foetal calf serum (10%) and six steel beads using the homogeniser Precellys®24 Dual (Bertin, France) at 5500 rpm for 20 s. DNA extraction was performed on 100 μL of tick homogenate, using the NucleoSpin® Tissue DNA extraction kit (Macherey-Nagel, Germany).
Lejal E., Chiquet J., Aubert J., Robin S., Estrada-Peña A., Rue O., Midoux C., Mariadassou M., Bailly X., Cougoul A., Gasqui P., Cosson J.F., Chalvet-Monfray K., Vayssier-Taussat M, & Pollet T. (2021). Temporal patterns in Ixodes ricinus microbial communities: an insight into tick-borne microbe interactions. Microbiome, 9, 153.
+ Open protocol
+ Expand
9

Single-cell NGS and Deep Sequencing

Check if the same lab product or an alternative is used in the 5 most similar protocols
NGS for a single cell suspension was carried out by STABVIDA and GeneWiz Companies. PCR products were treated and purified according to manufacturer's protocols. Samples were used for a library construction and sequenced with v3 chemistry in the lllumina MiSeq platform. Only variants represented in more than 1% were considered.
For deep sequencing of liver cells, total genomic DNA was extracted from the livers using a NucleoSpin tissue DNA extraction kit (Macherey-Nagel) according to the manufacturer’s instructions. A first PCR round was performed using primers that amplified 390 bp of the mouse Hao1 gene (containing the targeted region in the middle of the amplicon) carrying the universal adaptor M13 sequence (underlined) in their 5′ end (forward, 5′-GTTGTAAAACGACGGCCAGTAGACCAATGTTTGTCAGAGG-3′ and reverse, 5′-CACAGGAAACAGCTATGACCTAAAAGCATCCTAGGAAGGG-3′). Platinum Taq polymerase (Invitrogen) was used. Subsequently, a second PCR was performed using primers targeting the universal M13 sequence and adding unique MID (multiplex identifier) barcodes to each sample. Sample pools were NGS sequenced on the MiSeq platform (Illumina, San Diego, CA, USA) following the manufacturer’s protocol.
López-Manzaneda S., Ojeda-Pérez I., Zabaleta N., García-Torralba A., Alberquilla O., Torres R., Sánchez-Domínguez R., Torella L., Olivier E., Mountford J., Ramírez J.C., Bueren J.A., González-Aseguinolaza G, & Segovia J.C. (2020). In Vitro and In Vivo Genetic Disease Modeling via NHEJ-Precise Deletions Using CRISPR-Cas9. Molecular Therapy. Methods & Clinical Development, 19, 426-437.
+ Open protocol
+ Expand
10

Fecal DNA Extraction at 16 and 22 Days Post-Inoculation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fecal samples were collected on sterile tubes. Fresh feces were collected from each animal at 16 days post-inoculation (dpi) and 22 dpi. Fecal samples were stored in sterile tubes at −20 °C. Fecal genomic DNA was extracted using a Nucleospin tissue DNA extraction Kit (Macherey-Nagel, Hoerdt, France). Each DNA sample was eluted in 100 μl of sterile water. Genomic DNA quality (OD260/280 between 1.8 and 2.0) was measured with NanoDrop™ One (Thermo Scientific, Waltham, MA, USA).
Wu-Chuang A., Mateos-Hernandez L., Abuin-Denis L., Maitre A., Avellanet J., García A., Fuentes D, & Cabezas-Cruz A. (2024). Exploring the impact of breast cancer on colonization resistance of mouse microbiota using network node manipulation. Heliyon, 10(10), e30914.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!