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Amikacin

Manufactured by Bristol-Myers Squibb
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Amikacin is an antibiotic medication used to treat various types of bacterial infections. It is a lab equipment product used in clinical settings to identify and quantify the presence of Amikacin in biological samples.

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Lab products found in correlation

M199 medium, by Merck Group (3 mentions) Basal medium eagle vitamins, by Merck Group (3 mentions) Buprenorphine, by Reckitt Benckiser (2 mentions) Dneasy blood tissue kit, by Qiagen (1 mentions) Aripiprazole, by Bio-Techne (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Biosera (1 mentions) Biopterin, by Merck Group (1 mentions) Hygromycin b, by Carl Roth (1 mentions) D amphetamine, by Merck Group (1 mentions)

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Amikacin (Amikin, (3 citations)

6 protocols using amikacin

1

Promastigote Cultivation and 18S rRNA Sequencing

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Promastigotes were cultured in the M199 medium (Sigma−Aldrich, St. Louis, MO, United States) containing 10% heat-inactivated fetal bovine calf serum (FBS; Thermo Fisher Scientific, Waltham, MA, United States), supplemented with 1% Basal Medium Eagle vitamins (Sigma−Aldrich), 2% sterile urine and 250 μg/ml of amikacin (Bristol-Myers Squibb, New York, NY, United States).
Total genomic DNA was isolated from 10 ml of trypanosomatid cultures with the DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany) according to the manufacturer’s instructions. 18S rRNA gene was amplified using primers S762 and S763 [48 (link)], following the previously described protocol [13 (link)]. These PCR fragments were sequenced directly at Macrogen Europe (Amsterdam, Netherlands) as described previously [49 (link)]. The identity of species under study was confirmed by BLAST analysis [50 (link)].
Butenko A., Kostygov A.Y., Sádlová J., Kleschenko Y., Bečvář T., Podešvová L., Macedo D.H., Žihala D., Lukeš J., Bates P.A., Volf P., Opperdoes F.R, & Yurchenko V. (2019). Comparative genomics of Leishmania (Mundinia). BMC Genomics, 20, 726.
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2

Psychopharmacologic Perioperative Treatments

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Cocaine hydrochloride (Pharmacy of the Geneva University Hospitals) was dissolved in saline. Aripiprazole (Tocris, Bristol, UK) was suspended in 1 mL distilled water and 5% TWEEN. For perioperative care, amikacin (Bristol-Myers Squibb, Cham, Switzerland), cefazolin (Labatec Pharma, Meyrin, Switzerland) and buprenorphine (Reckitt Benckiser, Wallisellen, Switzerland) were dissolved in saline.
Arrondeau C., Urueña-Méndez G., Bellés L., Marchessaux F., Goutaudier R, & Ginovart N. (2023). Motor impulsivity but not risk-related impulsive choice is associated to drug intake and drug-primed relapse. Frontiers in Behavioral Neuroscience, 17, 1200392.
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3

Culturing L. mexicana Promastigotes and Differentiating Metacyclic and Amastigote Forms

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L. mexicana (MNYC/BZ/1962/M379) promastigotes were cultured in M199 medium (Sigma-Aldrich, St. Louis, USA), supplemented with 2 µg/ml biopterin (Sigma-Aldrich), 2 µg/ml Hemin (Jena Bioscience GmbH, Jena, Germany), 25 mM HEPES and 50 units/ml of penicillin/streptomycin (Life Technologies/Thermo Fisher Scientific, Carlsbad, USA), and 10% heat-inactivated fetal bovine serum (FBS, BioSera Europe, Nuaillé, France). Metacyclic promastigotes and amastigotes were differentiated as previously described [44 (link)]. Prior to in vivo analysis, Leishmania cell lines were passaged through mice. Freshly isolated promastigotes were cultured in M199 medium (Sigma-Aldrich) containing 10% heat-inactivated FBS (Life Technologies) supplemented with 1% Basal Medium Eagle vitamins (Sigma-Aldrich), 2% sterile human urine, and 250 μg/ml amikacin (Bristol-Myers Squibb, New York, USA). For mice infections, the pH of the cultivation medium was adjusted to 5.5.
Sádlová J., Podešvová L., Bečvář T., Bianchi C., Gerasimov E.S., Saura A., Glanzová K., Leštinová T., Matveeva N.S., Chmelová Ľ., Mlacovská D., Spitzová T., Vojtková B., Volf P., Yurchenko V, & Kraeva N. (2021). Catalase impairs Leishmania mexicana development and virulence. Virulence, 12(1), 852-867.
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4

Culturing L. mexicana Promastigotes and Evaluating Macrophage Infection

Cited 3 times
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The L. mexicana wild type promastigotes were cultured in the M199 medium (Sigma-Aldrich) containing 10% FBS supplemented with 1% Basal Medium Eagle vitamins (Sigma-Aldrich), 2% sterile urine, and 250 µg/mL Amikacin (Bristol-Myers Squibb, New York, NY, USA), and 200 µg/mL of Hygromycin B (Carl Roth GmbH) for BnSP-7-ecDHFR-HA mutant cell lines. Infection of the J774 macrophages was performed as described previously [49 (link),52 (link)]. In addition, infection was evaluated by counting Giemsa-stained slides as described previously [51 (link)]. All experiments were performed with two independent biological replicates and samples were analyzed in triplicate.
Podešvová L., Leštinová T., Horáková E., Lukeš J., Volf P, & Yurchenko V. (2020). Suicidal Leishmania. Pathogens, 9(2), 79.
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5

Pharmacological Preoperative Protocol

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d-Amphetamine (Sigma-Aldrich, Switzerland) and cocaine (Pharmacy of the University Hospital of Geneva) were dissolved in saline. For perioperative care, amikacin (Bristol-Myers Squibb, Cham, Switzerland), cefazolin (Labatec Pharma, Meyrin, Switzerland), and buprenorphine (Reckitt Benckiser, Wallisellen, Switzerland) were diluted in saline.
Bellés L., Dimiziani A., Herrmann F.R, & Ginovart N. (2021). Early environmental enrichment and impoverishment differentially affect addiction-related behavioral traits, cocaine-taking, and dopamine D2/3 receptor signaling in a rat model of vulnerability to drug abuse. Psychopharmacology, 238(12), 3543-3557.
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6

Amikacin Dosing in Severe Sepsis

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Adults (≥ 18 years) with severe sepsis or septic shock according to standard criteria admitted at the ED of the University Hospitals Leuven between 8 am and 5 pm on weekdays, were prospectively included from January 2013 to August 2014 [25] . This timeframe was chosen in function of availability of the research team. Written informed consent was obtained from each patient or relative. Patients with (1) age < 18 years, (2) pregnancy, (3) burns, (4) Amikacin treatment 2 weeks prior to inclusion, (5) known allergy to aminoglycosides, (6) a Do Not Resuscitate code, or (7) dialysis within 24 hours after admission were excluded. Patients were randomly assigned by envelope to a single dose of 15 mg/kg vs. 25 mg/kg, using total body weight (TBW) for dose calculation. The dose was limited to 1.2 g and 2 g in the 15 mg/kg vs. 25 mg/kg group for patients with a TBW higher than 80 kg. Amikacin (Amukin®Bristol-Myers Squibb) was given intravenously as a 30-minute infusion. At the discretion of the treating clinician, other antibiotics were prescribed in combination with Amikacin. Blood samples were collected in lithium-heparin tubes at 1, 6 and 24 hours after the onset of the first Amikacin infusion.
De Winter S., van Hest R., Dreesen E., Annaert P., Wauters J., Meersseman W., Van den Eede N., Desmet S., Verelst S., Vanbrabant P., Peetermans W, & Spriet I. (2021). Quantification and Explanation of the Variability of First-Dose Amikacin Concentrations in Critically Ill Patients Admitted to the Emergency Department: A Population Pharmacokinetic Analysis. European journal of drug metabolism and pharmacokinetics, 46(5).
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