All the experiments were performed after the protocol received approval by the Ethics Committee for Animal Use of the Instituto Politécnico Nacional/Escuela Nacional de Ciencias Biológicas (IPN/ENCB). All efforts were done to minimize the number of animals used and their suffering.
For IMQ-induced psoriasis, female BALB/c mice of 6–8 weeks old were shaved at their lower back, and except for the healthy group, all animals were treated topically on the back with 62.5 mg of IMQ cream (5%) (Aldara; 3M Pharmaceuticals) for six days or 12 mg of the IMQ cream on one ear [9 (link)].
For psoriasis TPA-induced model, female BALB/c mice of 6–8 weeks received 6 topical applications of 50 μg/mL TPA (Sigma- Aldrich, St. Louis, US) dissolved in acetone on shaved lower backs or in one ear [18 (link)].
As control groups we worked with healthy mice, mice treated with acetone (TPA vehicle) and psoriatic mice (induced with IMQ or TPA) treated with betamethasone (βMet). Each group consisted of 3 mice. In the ear model we measured the ear thickness using a digimatic thickness gauge (Mitutoyo, Illinois, US). All measurements were performed by the same person.
For IMQ-induced psoriasis, female BALB/c mice of 6–8 weeks old were shaved at their lower back, and except for the healthy group, all animals were treated topically on the back with 62.5 mg of IMQ cream (5%) (Aldara; 3M Pharmaceuticals) for six days or 12 mg of the IMQ cream on one ear [9 (link)].
For psoriasis TPA-induced model, female BALB/c mice of 6–8 weeks received 6 topical applications of 50 μg/mL TPA (Sigma- Aldrich, St. Louis, US) dissolved in acetone on shaved lower backs or in one ear [18 (link)].
As control groups we worked with healthy mice, mice treated with acetone (TPA vehicle) and psoriatic mice (induced with IMQ or TPA) treated with betamethasone (βMet). Each group consisted of 3 mice. In the ear model we measured the ear thickness using a digimatic thickness gauge (Mitutoyo, Illinois, US). All measurements were performed by the same person.