Evo 200 robot

Manufactured by Tecan

The EVO 200 robot is a liquid handling system designed for automated pipetting and sample processing in laboratory environments. It features a modular design and can be customized with a range of accessories and peripherals to suit diverse application needs. The core function of the EVO 200 is to perform precise and accurate liquid handling tasks in a reliable and automated manner.

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Lab products found in correlation

Q exactive, by Thermo Fisher Scientific (2 mentions)

4 protocols using evo 200 robot

Microsomal Stability Assay in High-Throughput

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Single time point microsomal stability was determined in a 96-well HTS format. Sample preparation was automated using a Tecan EVO 200 robot. High-resolution LC–MS (Thermo ZExactive) instrument was used to measure the percentage of compound remaining after incubation using a previously described method.^{40 (link)} Six standard controls were tested in each run: buspirone and propranolol (for short half-life), loperamide and diclofenac (for short to medium half-life), and carbamazepine and antipyrine (for long half-life). Briefly, the incubation consisted of a 0.5 mg/mL microsomal protein, a 1.0 μM drug concentration, and NADPH regeneration system (containing0.650 mM NADP⁺, 1.65 mM glucose 6-phosphate, 1.65 mM MgCl₂, and 0.2 unit/mL G6PDH) in 100 mM phosphate buffer at pH 7.4. The incubation was carried out at 37 °C for 15 min. The reaction was quenched by adding 555 μL of acetonitrile (~1:2 ratio) containing0.28 μM albendazole (internal standard). Sample acquisition and data analysis were done using a previously described method.^{40 (link)}

Manz T.D., Sivakumaren S.C., Ferguson F.M., Zhang T., Yasgar A., Seo H.S., Ficarro S.B., Card J.D., Shim H., Miduturu C.V., Simeonov A., Shen M., Marto J.A., Dhe-Paganon S., Hall M.D., Cantley L.C, & Gray N.S. (2020). Discovery and Structure-Activity Relationship Study of (Z)‑5-Methylenethiazolidin-4-one Derivatives as Potent and Selective Pan-phosphatidylinositol 5‑Phosphate 4‑Kinase Inhibitors. Journal of medicinal chemistry, 63(9), 4880-4895.

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High-throughput microsomal stability assay

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Single time point microsomal stability was determined in a 96-well HTS format. Sample preparation was automated using a Tecan EVO 200 robot. A high-resolution LC/MS (Thermo Q Exactive) instrument was used to measure the percentage of the compound remaining after incubation using a previously described method.^{47 (link)} Six standard controls were tested in each run: buspirone and propranolol (for short half-life), loperamide and diclofenac (for short to medium half-life), and carbamazepine and antipyrine (for long half-life). DMSO stock solutions (10 mM) of the drugs were first diluted to 10 μM in 1:2 MeCN/DI H₂O and then further diluted to 1 μM in the assay buffer. Briefly, the incubation consisted of 0.5 mg/mL microsomal protein, 1.0 μM drug concentration, and an NADPH regeneration system (containing 0.650 mM NADP+, 1.65 mM glucose 6-phosphate, 1.65 mM MgCl₂, and 0.2 U/mL G6PDH) in 100 mM phosphate buffer at pH 7.4. The incubation was carried out at 37 °C for 15 min.^{48 (link)} The reaction was quenched by adding 555 μL of acetonitrile (~1:2 ratio) containing 0.28 μM albendazole (internal standard). Sample acquisition and data analysis were done using a previously described method.^{47 (link)}

Liu L., Tang M., Pragani R., Whitby F.G., Zhang Y.Q., Balakrishnan B., Fang Y., Karavadhi S., Tao D., LeClair C.A., Hall M.D., Marugan J.J., Boxer M., Shen M., Hill C.P., Lai K, & Patnaik S. (2021). Structure-Based Optimization of Small Molecule Human Galactokinase Inhibitors. Journal of medicinal chemistry, 64(18), 13551-13571.

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High-Throughput Microsomal Stability Assay

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Single time point microsomal stability was determined in a 96-well HTS format. Sample preparation was automated using a Tecan EVO 200 robot. A high-resolution LC–MS (Thermo Q Exactive) instrument was used to measure the percentage of the compound remaining after incubation using a previously described method.^{31 (link)} Six standard controls were tested in each run: buspirone and propranolol (for short half-life), loperamide and diclofenac (for short to medium half-life), and carbamazepine and antipyrine (for long half-life). Briefly, the incubation consisted of 0.5 mg/mL microsomal protein, 1.0 μM drug concentration, and an NADPH regeneration system (containing 0.650 mM NADP⁺, 1.65 mM glucose 6-phosphate, 1.65 mM MgCl₂, and 0.2 unit/mL G6PDH) in 100 mM phosphate buffer at pH 7.4. The incubation was carried out at 37 °C for 15 min. The reaction was quenched by adding 555 μL of acetonitrile (~1:2 ratio) containing 0.28 μM albendazole (internal standard). Sample acquisition and data analysis were done using a previously described method.^{29 (link)}

Rai G., Urban D.J., Mott B.T., Hu X., Yang S.M., Benavides G.A., Johnson M.S., Squadrito G.L., Brimacombe K.R., Lee T.D., Cheff D.M., Zhu H., Henderson M.J., Pohida K., Sulikowski G.A., Dranow D.M., Kabir M., Shah P., Padilha E., Tao D., Fang Y., Christov P.P., Kim K., Jana S., Muttil P., Anderson T., Kunda N.K., Hathaway H.J., Kusewitt D.F., Oshima N., Cherukuri M., Davies D.R., Norenberg J.P., Sklar L.A., Moore W.J., Dang C.V., Stott G.M., Neckers L., Flint A.J., Darley-Usmar V.M., Simeonov A., Waterson A.G., Jadhav A., Hall M.D, & Maloney D.J. (2020). Pyrazole-Based Lactate Dehydrogenase Inhibitors with Optimized Cell Activity and Pharmacokinetic Properties. Journal of medicinal chemistry, 63(19), 10984-11011.

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Microsomal Stability Screening Automation

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Single time point microsomal stability was determined in a 96-well HTS format. Sample preparation was automated using Tecan EVO 200 robot. High Resolution LC/MS (Thermo Q Exactive™) instrument was used to measure the percentage of compound remaining after incubation using a previously described method.^{40 (link)} Six standard controls were tested in each run: buspirone and propranolol (for short half-life), loperamide and diclofenac (for short to medium half-life), and carbamazepine and antipyrine (for long half-life). 10 mM DMSO stock solutions of the drugs were first diluted to 10 μM in 1:2 MeCN:DI H₂O and then further diluted to 1 μM in assay buffer. Briefly, the incubation consisted of 0.5 mg/mL microsomal protein, 1.0 μM drug concentration, and NADPH regeneration system (containing 0.650 mM NADP+, 1.65 mM glucose 6-phosphate, 1.65 mM MgCl₂, and 0.2 unit/mL G6PDH) in 100 mM phosphate buffer at pH 7.4. The incubation was carried out at 37 °C for 15 min.^{36 (link)} The reaction was quenched by adding 555 μL of acetonitrile (~1:2 ratio) containing 0.28 μM albendazole (internal standard). Sample acquisition and data analysis was done using a previously described method.^{40 (link)}

Rohde J.M., Karavadhi S., Pragani R., Liu L., Fang Y., Zhang W., McIver A., Zheng H., Liu Q., Davis M.I., Urban D.J., Lee T.D., Cheff D.M., Hollingshead M., Henderson M.J., Martinez N.J., Brimacombe K.R., Yasgar A., Zhao W., Klumpp-Thomas C., Michael S., Covey J., Moore W.J., Stott G.M., Li Z., Simeonov A., Jadhav A., Frye S., Hall M.D., Shen M., Wang X., Patnaik S, & Boxer M.B. (2021). Discovery and optimization of 2H-1λ2-pyridin-2-one inhibitors of mutant isocitrate dehydrogenase 1 for the treatment of cancer. Journal of medicinal chemistry, 64(8), 4913-4946.

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