Dcc 2036

DCC-2036 (Rebastinib) was purchased from Selleck (Houston, TX). The compound was dissolved in DMSO at a stock concentration of 20 mM and stored at −20 °C. DCC-2036 (0 μM) means DMSO treatment with equivalent volume. Recombinant Human GAS6 (C-Fc) #C12W was purchased from Novoprotein (Suzhou, Jiangsu, China). Recombinant Mouse GAS6 Protein (His Tag) #58026-M08H was purchased from Sino Biological (Shanghai, China). Cycloheximide and MG132 were purchased from Sigma-Aldrich (St Louis, MO, USA). The following antibodies were used for immunoblotting: phospho-AXL (Y702) #5724, phospho-AKT (S473) #4060, AKT #4691,phospho-GSK3β (S9) #5558, GSK3β #12456, β-actin #4970, purchased from Cell Signaling Technology (Beverley, MA); ALDH1A1 #15910-1-AP, OCT4 #11263-1-AP, KLF4 #11880-1-AP, SOX2 #11064-1-AP, KLF5 #21017-1-AP, NANOG #14295-1-AP, AXL #13196-1-AP, MET #25869-1-AP, FGF-BP1 #25006-1-AP purchased from Proteintech (Wuhan, Hubei, China); NF-κB (P65) #ab16502, purchased from Abcam; FGF-BP1 #AF1593 purchased from R&D; phospho-KLF5 (S303) #AF7042, phospho-AXL (Tyr702) # AF8523 purchased from Affinity (Changzhou, Jiangsu, China). APC anti-mouse CD24 #138506 purchased from Biolegend (San Diego, CA, USA), PE Rat anti-mouse CD44 #553134, PE Mouse Anti-Human CD24 #555428, APC Mouse Anti-Human CD44 #559942 purchased from BD Pharmingen (Franklin Lakes, New Jersey, USA).

Imatinib, nilotinib, dasatinib, bafetinib, ponatinib, DCC-2036, GNF-2, GNF-5, EPZ005687, XAV-939, and FH535 were purchased from Selleckchem (Houston, TX, USA). PHA665752 was purchased from MedChemExpress, LLC (Princeton, NJ, USA). U0126, LY294002, and SP600125 were purchased form Wako (Tokyo, Japan). These reagents were dissolved in dimethyl sulfoxide and diluted in phosphate-buffed saline (PBS; 0.05 M, pH 7.4), filtrated through syringe filters (0.45 μm, IWAKI GLASS, Tokyo, Japan), and used for various assays described below.