Unless stated otherwise, all chemical reagents were purchased from either Applichem (Darmstadt, Germany) or Sigma-Aldrich (Munich, Germany), and all cell culture reagents were purchased from either PAA laboratories (Cölbe, Germany) or Biochrom AG (Berlin, Germany). Rabbit polyclonal PDZK1 antibody (PAB15564) was from Abnova (Jhongli City, Taoyuan County, Taiwan), rabbit polyclonal NHE3 antibody (NHE31-A) was from Alpha diagnostic (San Antonio, USA), and rabbit polyclonal β-actin antibody (ab8227) was from Abcam (Cambridge, UK).
Cell culture reagents
Manufactured by GE Healthcare
Sourced in United States, Germany
Cell culture reagents are a set of products designed to support the growth and maintenance of cells in a laboratory setting. These reagents provide the necessary components, such as nutrients, growth factors, and buffers, to create an optimal environment for cultivating a variety of cell types. The core function of these reagents is to enable researchers and scientists to establish and sustain cell cultures for various applications, including cell-based research, drug development, and tissue engineering.
Automatically generated - may contain errors
Lab products found in correlation
Dihydroethidium, by Thermo Fisher Scientific (3 mentions)
β actin antibody ab8227, by Abcam (2 mentions)
Pdzk1 antibody pab15564, by Abnova (2 mentions)
Nhe3 antibody nhe31 a, by Alpha Diagnostic (2 mentions)
Bicuculline, by Bio-Techne (2 mentions)
Lipofectamine 3000, by Thermo Fisher Scientific (2 mentions)
Acetylated lysine, by Cell Signaling Technology (1 mentions)
Suramin, by Merck Group (1 mentions)
Cleaved and total caspase 3, by Cell Signaling Technology (1 mentions)
Anti flag m2 magnetic bead, by Merck Group (1 mentions)
Aicar, by Merck Group (1 mentions)
Dithiothreitol (dtt), by Merck Group (1 mentions)
N6022, by Merck Group (1 mentions)
3 4 5 dimethyl 2 thiazolyl 2 5 diphenyl 2 h tetrazolium bromide mtt, by Sangon (1 mentions)
Attractene, by Thermo Fisher Scientific (1 mentions)
Fluor de lys sirt1 fluorometric drug discovery assay kit, by Enzo Life Sciences (1 mentions)
Muse count viability assay kit, by Merck Group (1 mentions)
Protein a magnetic bead, by Merck Group (1 mentions)
Foxo1, by Cell Signaling Technology (1 mentions)
Compound c, by Merck Group (1 mentions)
8 protocols using cell culture reagents
1
Antibody Reagents for Protein Expression
2
Antibody Reagents for Protein Expression
3
AMPK-p53 Signaling Modulation in Cell Assays
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Cell culture reagents were purchased from GE and fetal bovine serum (FBS) was purchased from PAN-Biotech; DTT was purchased from Millipore (Massachusetts, USA); Compound C, AICAR, Resveratrol, Suramin, SNAP, and ODQ were purchased from Sigma-Aldrich; N6022, Pifithrin-α, VO-Ohpic trihydrate, AS1842856, and MK 2206 were from MCE. Hydrogen peroxide (H2O2) was from Sinopharm Chemical Reagent; 3-(4,5-dimethyl-2-thiazolyl)−2,5-diphenyl-2-H-tetrazolium bromide (MTT) was purchased from Sangon Biotech; Lipofectamine® 3000 and Attractene were from Thermo Fisher Scientific and Qiagen, respectively. Fluor-de-Lys SIRT1 fluorometric drug discovery assay kit was from Enzo Life Sciences; Hydrogen Peroxide Assay Kit, Nitric Oxide Assay Kit, RIPA lysis buffer, Bicinchoninic Acid assay Kit, and Nuclear and Cytoplasmic Protein Extraction Kit were from Beyotime; Super ECL Prime was from US Everbright®Inc. The mRNA extraction kit was from Bioteke. Protein A Magnetic Beads and the Muse Count & Viability Assay Kit were purchased from Millipore. Anti-Flag M2 Magnetic Beads was from Sigma-Aldrich. Antibodies for phospho- and total-AMPK, acetyl- and total-p53, SIRT1, Cleaved- and total-Caspase-3, FOXO1, PTEN, β-actin, and acetylated-Lysine were from Cell Signaling Technology; antibodies for P21 was from Abcam; antibodies for Prdx2 was from Santa Cruz Biotechnology.
4
Reagent Sourcing for Cell Research
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Bicuculline was purchased from Tocris (Minneapolis, MN); pancuronium bromide from Astra Pharmaceutical Products (Westborough, MA); NaHS from Cayman Chemical (Ann Arbor, MI); dihydroethidium from Life Technologies (Thermo Fisher Scientific, Waltham, MA); and cell culture reagents were from GE Healthcare Life Sciences (Pittsburg, PA). All other reagents were purchased from Sigma (St. Louis, MO).
5
Fluorescence Imaging of Breast Cancer Cells
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Cell culture reagents were obtained from Hyclone (GE, Boston, MA, USA) unless otherwise noted. MDA-MB-231 breast tumor cells were obtained from the American Type Culture Collection and maintained in Dulbecco′s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum. For imaging assays, cells were plated in 12-well plates (Corning, Corning, NY, USA) and allowed to grow to ~50% confluency before being treated with solutions of SPcs in media (1% dimethylsulfoxide). Images were collected with a VWR Inverted Fluorescence Microscope, Moticam 5.0 (Motic, Richmond, BC, Canada), and TRITC filter set (λex = 540 nm ± 12 nm, λem = 605 nm ± 27 nm).
6
Reagent Sourcing for Cell Research
7
Extraction and Characterization of Ulva Derivatives
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The chemicals and reagents were acquired from Sigma Chemical (St. Louis, MO, USA). Cell
culture reagents were obtained from GE Healthcare Life Sciences (Marlborough, MA, USA). To
measure cytokines and immunoglobulins (Ig), enzyme-linked immunosorbent assay (ELISA) kits
were purchased from eBioscience, Inc. (San Diego, CA, USA). Reagents and antibodies for
immunohistochemical (IHC) staining were procured from Vector Laboratories, Inc. (Newark,
CA, USA) and Thermo Fisher Scientific Inc. (Waltham, MA, USA). The Ulvasp. used in this study was supplied by Professor Chorng-Liang Pan of the Department of
Food Science at National Taiwan Ocean University (Keelung, Taiwan), transformed into a
powder form using an electric grinder, and filtered through a 40-mesh sieve. The process
for preparing UP and UO followed the method described in a previous study [14 ]. However, in this study, a commercial enzyme called
Cellulase AP3 (2000 U/g; Amano Enzyme, Aichi, Japan) was used for the hydrolysis of UP.
After extraction of UP, the residues of Ulva were collected and
lyophilized as UR powder.
culture reagents were obtained from GE Healthcare Life Sciences (Marlborough, MA, USA). To
measure cytokines and immunoglobulins (Ig), enzyme-linked immunosorbent assay (ELISA) kits
were purchased from eBioscience, Inc. (San Diego, CA, USA). Reagents and antibodies for
immunohistochemical (IHC) staining were procured from Vector Laboratories, Inc. (Newark,
CA, USA) and Thermo Fisher Scientific Inc. (Waltham, MA, USA). The Ulvasp. used in this study was supplied by Professor Chorng-Liang Pan of the Department of
Food Science at National Taiwan Ocean University (Keelung, Taiwan), transformed into a
powder form using an electric grinder, and filtered through a 40-mesh sieve. The process
for preparing UP and UO followed the method described in a previous study [14 ]. However, in this study, a commercial enzyme called
Cellulase AP3 (2000 U/g; Amano Enzyme, Aichi, Japan) was used for the hydrolysis of UP.
After extraction of UP, the residues of Ulva were collected and
lyophilized as UR powder.
8
Investigating Oxidative Stress Modulators
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Cell culture reagents were purchased from GE Healthcare Life Sciences. Matrigel was obtained from Corning. Human TNF‐α was from R&D Systems. CellROX and dihydroethidium were from Life Technologies (Thermo Fisher Scientific). GKT137831 was purchased from MedKoo Biosciences, Inc. D, L‐sulforaphane and all other reagents were from Sigma.
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