The D-loop assay essentially was performed in 50 mM KCl and as described earlier (11 (link), 42 , 49 (link)), except that Cy5-labeled 90-mer ssDNA purchased from Integrated DNA Technologies (IDT) was used (Table 1 ). The 90-mer is homologous to the region of 1932–2022 bp within the pBluescript II SK(-) plasmid DNA (50 (link)). Fluorescence image capture and analysis were conducted on an Odyssey CLx Imaging System equipped with Image Studio 5.0 software (LI-COR).
Cy5 labeled 90 mer ssdna
Manufactured by Integrated DNA Technologies
Cy5-labeled 90-mer ssDNA is a single-stranded DNA molecule with a length of 90 nucleotides and a Cy5 fluorescent label attached. Cy5 is a cyanine dye that emits light in the red region of the visible spectrum. The core function of this product is to serve as a labeled nucleic acid probe or target for various research and diagnostic applications that require detection or quantification of specific DNA sequences.
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2 protocols using cy5 labeled 90 mer ssdna
1
D-loop Assay with Cy5-labeled ssDNA
2
D-loop Formation Assay Protocol
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The D-loop assay essentially was performed in 50 mM KCl and as described earlier (11, 42, 49) , except that Cy5-labeled 90-mer ssDNA purchased from Integrated DNA Technologies (IDT) was used (Table 1). The 90mer is homologous to the region of 1932-2022 bp within the pBluescript II SK(-) plasmid DNA (50) . Fluorescence image capture and analysis was conducted on an Odyssey CLx Imaging System equipped with Image Studio 5.0 software (LI-COR).
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