Ultrapure millipore direct q 3uv r

Stock solutions at a concentration of 1.0 ppm (mg/l) for zinc, 1.5 ppm for copper, 1.5 ppm for manganese were prepared by dilution the standard solutions at a concentration of 1000 ppm (Merck. Germany) in 0.5% nitric acid; prepared by diluting 65% Suprapur nitric acid (Merck, Darmstadt, Germany) in deionized water by Ultrapure Millipore Direct-Q-R 3UV (Merck, Darmstadt, Germany) with a resistivity of 18.2 MΩ∙cm.

All reagents were of Suprapur Grade and were bought from Merck (Darmstadt, Germany). High purity deionized water (resistivity of 18.2 MWcm) obtained using an Ultrapure Millipore Direct-Q-R 3UV (Millipore, Bedford, MA, USA) was used throughout the analysis. Quartz crucibles and polypropylene recipients were applied for the digestion and digest storage, respectively.

The standards of 6-gingerol and 10-shogaol were purchased from Sigma-Aldrich (St. Louis, MO, USA). Water, formic acid, and acetonitrile of spectroscopic grade were produced by J. T. Baker (Center Valley, PA, USA).
Nitric (V) acid (65%) and hydrochloric acid (36%) were of Suprapur Grade and were bought from Merck (Darmstadt, Germany). High purity deionized water (resistivity 18.2 MΩcm) obtained using an Ultrapure Millipore Direct-Q-R 3UV (Merck, Darmstadt, Germany) was used throughout the analysis. Quartz crucibles and polypropylene recipients were applied for the digestion and storage of digests, respectively.
A series of standard solutions of Ca, Cd, Cr, Cu, Fe, K, Mg, Mn, Na, Ni, Pb, and Zn at a concentration of 1000 mg/L (Merck, Germany) was used to plot the calibration curve for each element.
Diethyl ether and silica gel (230–400 mesh) applied in the GC-MS analysis of the extracts were produced by Merck (Germany). A C9–C25 hydrocarbon solution used as a reference mixture for GC-MS analysis was purchased from Sigma-Aldrich.

The samples were accurately weighted, quantitatively transferred into CX-100 Teflon vessels, and mineralized in TOPWave apparatus (Analytik Jena, Germany) with an oxidative mixture composed of 69% nitric acid (Suprapure Merck Darmstadt, Germany) and deionized water (Ultrapure Millipore Direct-Q-R 3UV Merck) in a ratio of 2:1 (v/v).^{11 (link)} The conditions of the process are presented in Table 2. The supernatant was taken for ICP-OES analysis and solid residues were assessed based on microscopic analysis.

Stock solutions at concentration of 3.0 ppb (μg/l) for cadmium, 35 ppb for chromium, 60 ppb for nickel and 30 ppb for lead were prepared by dilution the standard solutions at a concentration of 1000ppm (Merck, Darmstadt, Germany) in 0.5% nitric acid; prepared by diluting 65% Suprapur nitric acid (Merck, Darmstadt, Germany) in deionized water by Ultrapure Millipore Direct-Q-R 3UV (Merck, Darmstadt, Germany) with a resistivity of 18.2 MΩ∙cm.

Murashige & Skoog Medium Vitamin Mixture (MS), auxins: IBA (>98.0%), 2,4-D (>96%), and cytokinins: KIN (>98%), BA (>99%), 2iP (>98%), and TDZ (>95%) were purchased from Duchefa Biochemie B.V. (Haarlem, the Netherlands). Acetonitrile (ACN) and trifluoroacetic acid (TFA) of at least pro-analysis-grade were supplied by Merck (Darmstadt, Germany). 2-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), Folin–Ciocalteu reagent, trolox, aluminum chloride, and standards of analytes: ChA (≥95.0%), 3,5CQA (≥95.0%), rutin (≥94.0%), UA (≥90.0%), and OA (≥97.0%) were purchased in Sigma (St. Louis, MO, USA). CO standard was obtained by distillation of C. acaulis roots in the Deryng apparatus. The purity of the standard (96.2%) was evaluated using GC-MS, and the identity was confirmed by IR, Raman, and NMR spectroscopy according to the methodology published previously [13 (link)]. Water was deionized and purified using Ultrapure Millipore Direct-Q^® 3UV-R (Merck, Darmstadt, Germany).

Chlorogenic acid (≥95%), neoChlorogenic acid (≥98%), cryptoChlorogenic acid (≥98%), 3,5-di-caffeoylquinic acid (≥95%), trifluoroacetic acid (≥99%), and HPLC-grade methanol and acetonitrile were purchased from Sigma Aldrich (St. Louis, MO, USA). Water for HPLC was purified by Ultrapure Milli-pore Direct-Q^®3UV–R (Merck Millipore, Billerica, MA, USA). The COx with 96.2% purity was obtained by the distillation of C. acaulis roots in the Deryng apparatus. The identity and purity of the compound were confirmed in accordance with previously established methodology [30 (link)]. The ointment with mupirocin (Mupirox, Pharmaswiss Ceska Republika, Prague, Czech Republic) and clotrimazole (Clotrimazolum Hasco, Hasco-Lek S.A., Wroclaw, Poland) was purchased from a local pharmacy.