For cell cycle analysis, HUES6 cells were individualized using accutase 1:4 in PBS and fixed in 70% ethanol overnight. For BrdU detection, cells were incubated with 10 μM BrdU for 30 minutes before collection. After incubation with 0.5 mg/ml pepsin cells were treated with 2 N HCl for 20 minutes, washed and incubated with a rat-antiBrdU antibody (AbD serotec) for 45 minutes followed by incubation with goat anti-rat-FITC secondary antibody (Southern Biotech) for 30 minutes. Cells were then resuspended in PBS containing 50 μg/ml of propidium iodide and 0.5 mg/ml of RNaseA. Flow cytometry analyses were conducted using a LSRII cytometer (BD Bioscience) and data was analyzed using FlowJo software.
Goat anti rat fitc secondary antibody
Manufactured by Southern Biotech
The Goat anti-rat-FITC secondary antibody is a laboratory reagent used in immunoassays and flow cytometry applications. It is a fluorescently labeled antibody that binds to rat primary antibodies, allowing for the detection and visualization of target proteins or cells.
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Lab products found in correlation
2 protocols using goat anti rat fitc secondary antibody
1
Cell Cycle Analysis of HUES6 Cells
2
Cell Cycle Analysis of HUES6 Cells
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