Bio majesty jca bm 6050

Six nonpregnant, nonlactating Holstein cows were enrolled in the study. The average age of cows was 4.7 yr, and average body weight (BW) and body condition score [14 (link)] were 735 kg and 3.5, respectively. Cows were maintained in individual tie stalls.
Before 48 h fasting, cows were fed a constant amount of grass hay (about 1.0% of BW/d, DM basis) twice daily (0930 and 1630). Animals had free access to water and a trace mineralized salt block throughout the experimental period.
Blood samples were collected from the tail veins at d 0 (before fasting, 0900 h) and d 2 (after 48 h fasting). After sampling, blood samples (heparin tube) were immediately cooled on crushed ice. After centrifugation (1,000×g for 20 min at 4°C), separated plasma was immediately frozen and stored at −30°C until analyses. Plasma concentrations of NEFA, β-hydroxybutyric acid (BHBA), and glucose were determined using a clinical biochemistry autoanalyzer (BioMajesty JCA-BM 6050; JEOL Ltd., Tokyo, Japan).
Liver biopsies were performed at d 0 (before fasting) and d 2 (after 48 h fasting), following a procedure described previously [15 (link)]. Liver samples were rinsed with ice-cold saline solution and immediately frozen in liquid nitrogen. Samples were stored at −80°C until gene and protein expression analyses.

Blood was collected from the mice, incubated at 25 °C for 30 min and at 4 °C for 16 h, centrifuged at 1,000 × g for 30 min at 4 °C and the serum collected and stored at −80 °C until use. Biochemical blood tests were conducted with an automated analyser (Bio Majesty JCA-BM6050; JEOL, Tokyo, Japan). TG levels in the liver were quantified by colorimetric assay using the Triglyceride Quantification Assay kit according to the manufacturer’s protocol.