Prism ver 8.4.2 for windows

Mann–Whitney U tests were used to assess differences between unpaired groups with a nonparametric distribution. One-way analysis of variance (ANOVA) followed by the Kruskal–Wallis test was used for nonparametric multiple comparisons. Chi-squared tests or Fisher’s exact tests were used to analyze categorical data. The Pearson product-moment correlation coefficient was used for the assessment of correlations. In the survival analysis, the end point of OS was defined as the time from the day of treatment initiation until death from any cause. PFS was determined as the time from the day of treatment initiation until disease progression assessed by mRECIST1.1 or death, whichever occurred first. Differences in OS and PFS were analyzed by the Kaplan–Meier method and log-rank test. Factors associated with improved PFS were analyzed using univariate and multivariate Cox proportional hazards regression models. When dichotomizing factors, we used each median value as the cut-off value. Otherwise, the statistical analyses used are indicated in the figure legends. A p-value < 0.05 was considered to indicate statistical significance. Prism ver.8.4.2 for Windows (GraphPad Prism, RRID:SCR_002798, San Diego, CA, USA) and JMP^® 13 (SAS Institute Inc. RRID:SCR_014242, Cary, NC, USA) were used for the analyses.

The Mann–Whitney U test was used to evaluate differences between unpaired groups in a nonparametric distribution. For nonparametric multiple comparisons, one-way analysis of variance (ANOVA) followed by the Kruskal–Wallis test was used. For the analysis of categorical data, the chi-square test or Fisher’s exact test was used. Pearson product-moment correlation coefficients were used to assess correlations. For survival analysis, OS was defined as the endpoint from the start of treatment until death from any cause. The Kaplan–Meier and log-rank tests were used to analyzed differences of OS and PFS. Univariate and multivariate Cox proportional hazards regression models were used to identify factors associated with PFS. Statistical significance was p values < 0.05. JMP^® 13 (SAS Institute Inc. RRID:SCR_014242, Cary, NC, USA) and Prism ver. 8.4.2 for Windows (GraphPad Prism, RRID:SCR_002798, San Diego, CA, USA) were used for analysis.