Custom-made, affinity-purified antibodies generated against two short amino acid stretches of rat Jacob (285–299 and 400–314, Jb150) have been described previously [14 (link), 21 (link), 22 (link)]. The following commercial primary antibodies were used in the present study: mouse monoclonal anti-MAP2 (Sigma-Aldrich, M4403), anti-MAP2, Alexa Fluor 488 conjugated (Merck, MAB 3418x), mouse monoclonal anti-LaminB1 (ProteinTeck, 3C10G12), rabbit polyclonal anti-CRM1 (ProteinTeck), mouse monoclonal anti-MBP (NEB), mouse monoclonal anti-GFP (BioLegend, B34), and rabbit polyclonal anti-tRFP (EVROGEN). For imaging and WB analysis, the following secondary antibodies were used: anti-rabbit, anti-mouse secondary antibodies conjugated with Alexa Fluor 488, 561 or 647 (Life Technologies), peroxidase-conjugated anti-mouse IgG (Dianova) and anti-rabbit IgG (Dianova).
Peroxidase conjugated anti mouse igg
Manufactured by Dianova
Peroxidase-conjugated anti-mouse IgG is a secondary antibody used in various immunoassay techniques. It binds to mouse immunoglobulin G (IgG) and is conjugated with the enzyme horseradish peroxidase, which can be detected through chromogenic or chemiluminescent substrates.
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2 protocols using peroxidase conjugated anti mouse igg
1
Antibody Generation and Characterization for Jacob
2
Antibody Characterization and Protein Detection
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Custom-made, affinity-purified antibodies generated against two short amino acid stretches of rat Jacob (285-299 and 400-314, Jb150) have been described previously [14, 21, 22] . The following commercial primary antibodies were used in the present study:
mouse monoclonal anti-MAP2 (Sigma-Aldrich, M4403, mouse monoclonal anti-LaminB1
(ProteinTeck, 3C10G12), rabbit polyclonal anti-CRM1 (ProteinTeck), mouse monoclonal anti-MBP (NEB), mouse monoclonal anti-GFP (BioLegend, B34), and rabbit polyclonal anti-tRFP (EVROGEN). For imaging and WB analysis, the following secondary antibodies were used: anti-rabbit, anti-mouse secondary antibodies conjugated with Alexa Fluor 488, 561 or 647 (Life Technologies), peroxidase-conjugated anti-mouse IgG (Dianova)
and anti-rabbit IgG (Dianova).
mouse monoclonal anti-MAP2 (Sigma-Aldrich, M4403, mouse monoclonal anti-LaminB1
(ProteinTeck, 3C10G12), rabbit polyclonal anti-CRM1 (ProteinTeck), mouse monoclonal anti-MBP (NEB), mouse monoclonal anti-GFP (BioLegend, B34), and rabbit polyclonal anti-tRFP (EVROGEN). For imaging and WB analysis, the following secondary antibodies were used: anti-rabbit, anti-mouse secondary antibodies conjugated with Alexa Fluor 488, 561 or 647 (Life Technologies), peroxidase-conjugated anti-mouse IgG (Dianova)
and anti-rabbit IgG (Dianova).
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