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Enhanced dab kit

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The Enhanced DAB kit is a laboratory reagent used for immunohistochemistry and immunocytochemistry applications. It provides a chromogenic detection system for visualizing target proteins in biological samples.

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Lab products found in correlation

Immuno block reagent, by Bio SB (2 mentions) Mmp 2, by Thermo Fisher Scientific (1 mentions) Mmp 9, by Thermo Fisher Scientific (1 mentions) Jagged2, by Abcam (1 mentions)

2 protocols using enhanced dab kit

1

Immunohistochemical Staining for Ki67

Cited 1 time
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Immunohistochemical staining protocols were based on our previous study 41 (link). Briefly, paraffin sections were treated with 3% H2O2 for 10 minutes and incubated with Immuno-Block reagent (BioSB) for 30 minutes at room temperature, followed by incubation with the primary antibody specifically against Ki67 (Abcam) and secondary antibody. Immunoreactive signal was visualized by enhanced DAB kit (Abcam).
Li Y.C., Chen C.H., Chang C.L., Chiang J.Y., Chu C.H., Chen H.H, & Yip H.K. (2021). Melatonin and hyperbaric oxygen therapies suppress colorectal carcinogenesis through pleiotropic effects and multifaceted mechanisms. International Journal of Biological Sciences, 17(14), 3728-3744.
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2

IHC and IF Staining of MMP-2, MMP-9, Jagged2, Notch

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In detail, for those of IHC and IF staining, rehydrated paraffin sections were first treated with 3% H2O2 for 30 minutes and incubated with Immuno-Block reagent (BioSB, Santa Barbara, CA, USA) for 30 minutes at room temperature. Sections were then incubated with primary antibodies specifically against MMP-2 (1:200, Invitrogen, CA, USA), MMP-9 (1:200, Invitrogen, CA, USA), Jagged2 (1:100, Abcam, Cambridge, UK) and Notch (1:150, Abcam, Cambridge, UK), while sections incubated with the use of irrelevant antibodies served as controls. Immunoreactive were visualized by enhanced DAB kit (Abcam).
Chen Y.T., Huang C.R., Chang C.L., Chiang J.Y., Luo C.W., Chen H.H, & Yip H.K. (2020). Jagged2 progressively increased expression from Stage I to III of Bladder Cancer and Melatonin-mediated downregulation of Notch/Jagged2 suppresses the Bladder Tumorigenesis via inhibiting PI3K/AKT/mTOR/MMPs signaling. International Journal of Biological Sciences, 16(14), 2648-2662.
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