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Ultra lowattachment 24 wells plates

Manufactured by Corning
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The Corning Ultra LowAttachment 24-wells plates are designed for cell culture applications that require minimizing cell attachment to the plate surface. These plates feature a specialized hydrophilic, non-toxic, and non-pyrogenic coating that prevents cell adhesion, promoting the formation of spheroids, embryoid bodies, and other 3D cell culture models.

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Lab products found in correlation

Lipofectamine 3000 reagent, by Thermo Fisher Scientific (1 mentions) Cignal reporter assay, by Qiagen (1 mentions) 17β estradiol, by Merck Group (1 mentions) Basic fibroblast growth factor (bfgf), by Solarbio (1 mentions) Ix53 dp80, by Olympus (1 mentions) Epidermal growth factor (egf), by Solarbio (1 mentions) Dmem f12, by Thermo Fisher Scientific (1 mentions)

4 protocols using ultra lowattachment 24 wells plates

1

Generation of Gliomaspheres from Primary Astrocytes

Cited 1 time
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Primary newborn astroglial cells were isolated and maintained as
previously described78 . For
gliomasphere preparation, astroglial cells were plated at 20-30% confluence in
6-well plates in 2 ml astrocyte medium (DMEM medium supplemented with 10% FBS,
glutamine and antibiotics). The next day cells were transduced with lentiviral
vectors coding for oncogenes, or with empty vector as negative controls. After
24 hours (day 3), transduced astrocytes were switched to NSC medium (DMEM/F12
supplemented with 100X N2, 20 ng/ml mEGF, 20 ng/ml mbFGF, glutamine, and
antibiotics). Spheres arising from the cell monolayer were evident after
approximately 3 weeks. Sphere passaging was performed as described
previously74 (link).
To evaluate self-renewal properties (Extended Data Fig. 7d and 9b),
gliomaspheres were dissociated to single cells and replated in Ultra Low
Attachment 24-wells plates (Corning), at the concentration of 2,000 cells per
well; fully gliomaspheres were counted by visual inspection.
Castellan M., Guarnieri A., Fujimura A., Zanconato F., Battilana G., Panciera T., Sladitschek H.L., Contessotto P., Citron A., Grilli A., Romano O., Bicciato S., Fassan M., Porcù E., Rosato A., Cordenonsi M, & Piccolo S. (2020). Single-cell analyses reveal YAP/TAZ as regulators of stemness and cell plasticity in Glioblastoma. Nature cancer, 2(2), 174-188.
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2

Generation of Gliomaspheres from Primary Astrocytes

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary newborn astroglial cells were isolated and maintained as
previously described78 . For
gliomasphere preparation, astroglial cells were plated at 20-30% confluence in
6-well plates in 2 ml astrocyte medium (DMEM medium supplemented with 10% FBS,
glutamine and antibiotics). The next day cells were transduced with lentiviral
vectors coding for oncogenes, or with empty vector as negative controls. After
24 hours (day 3), transduced astrocytes were switched to NSC medium (DMEM/F12
supplemented with 100X N2, 20 ng/ml mEGF, 20 ng/ml mbFGF, glutamine, and
antibiotics). Spheres arising from the cell monolayer were evident after
approximately 3 weeks. Sphere passaging was performed as described
previously74 (link).
To evaluate self-renewal properties (Extended Data Fig. 7d and 9b),
gliomaspheres were dissociated to single cells and replated in Ultra Low
Attachment 24-wells plates (Corning), at the concentration of 2,000 cells per
well; fully gliomaspheres were counted by visual inspection.
Castellan M., Guarnieri A., Fujimura A., Zanconato F., Battilana G., Panciera T., Sladitschek H.L., Contessotto P., Citron A., Grilli A., Romano O., Bicciato S., Fassan M., Porcù E., Rosato A., Cordenonsi M, & Piccolo S. (2020). Single-cell analyses reveal YAP/TAZ as regulators of stemness and cell plasticity in Glioblastoma. Nature cancer, 2(2), 174-188.
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3

Estrogen Signaling Pathway Activation

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Cells were transfected with 1μg mixture of an inducible ERE-responsive firefly luciferase construct (kindly provided by Dr. De Bortoli, University of Torino) and a constitutively expressing Renilla luciferase construct (40:1) (Cignal Reporter Assay, Qiagen) using Lipofectamine 3000 reagent (Life Technologies) according to the manufacturer's instructions. Cells were seeded into ultra-low attachment 24 wells plates (Corning) at a density of 100,000 cells per well. After 48 hours from transfection, cells were treated for 6 hours with either vehicle or 10 nM 17-β-Estradiol (E2758, Sigma) and the reporter activity was measured by luminescence. Values were normalized to Renilla luciferase activity; data are presented as relative luciferase values.
Petrelli A., Carollo R., Cargnelutti M., Iovino F., Callari M., Cimino D., Todaro M., Mangiapane L.R., Giammona A., Cordova A., Montemurro F., Taverna D., Daidone M.G., Stassi G, & Giordano S. (2014). By promoting cell differentiation, miR-100 sensitizes basal-like breast cancer stem cells to hormonal therapy. Oncotarget, 6(4), 2315-2330.
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4

Ovarian Cancer Tumorsphere Cultivation

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Single-cell suspensions of ovarian cancer cells (4 × 102 cells/mL) were plated on ultra-low attachment 24 wells plates (Corning, America) and cultured in phenol red-free DMEM/F12 (Gibco, America) containing B27 supplement (Gibico, America, #12587010) and 20 ng/mL epidermal growth factor (EGF, Solarbio, China, #P00033), 20 ng/mL basic fibroblast growth factor (bFGF, Solarbio, China, #P00032), and 5 μg/mL insulin (Solarbio, China, #I8040). Tumorsphere were visualized under a phase-contrast microscope (Olympus, America, IX53 + DP80).
Chen Y., Qiang Y., Fan J., Zheng Q., Yan L., Fan G., Song X., Zhang N., Lv Q., Xiong J., Wang J., Cao J., Liu Y., Xiong J., Zhang W, & Li F. (2024). Aggresome formation promotes ASK1/JNK signaling activation and stemness maintenance in ovarian cancer. Nature Communications, 15, 1321.
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