Anti rabbit or anti mouse igg h l dylight

Cell pellets were incubated with 1X SDS sample buffer and then lysed by sonication. Lysates were cleared by centrifugation and analyzed by SDS-PAGE followed by Western blotting. The following primary antibodies were used: anti-p53 DO-1 (Santa Cruz Biotechnology) that recognizes epitopes in the region containing residues 11–25, anti-p53 pAB1801 (Santa Cruz Biotechnology) that recognizes epitopes in the region containing residues 32–79, anti-p53 C-terminal antibody (Sigma-Aldrich) that recognizes epitopes in the region containing residues 334–383, anti-GAPDH (Cell Signaling Technology), anti-OCT3/4 (Santa Cruz Biotechnology), anti-SOX2 (Abcam), anti-NANOG (Santa Cruz Biotechnology), anti-Tubulin (Millipore Sigma) and anti-BBS9 (Millipore Sigma). Secondary antibodies used were anti-rabbit or anti-mouse IgG (H + L) (Dylight 680 or 800 conjugates) (Cell Signaling Technology). The membranes were scanned with the Odyssey infrared imaging system (LI-COR Biosciences).