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3 protocols using thionyl chloride

1

Immobilization of Burkholderia cepacia Lipase

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Burkholderia cepacia lipase (BCL) powder was purchased from Amano Enzyme Inc. (Nagoya, Japan) and used without further purification. Multi-walled carbon nanotubes (40–60 nm diameter, purity >95%) were obtained from Nanotech Port Co. Ltd. (Shenzhen, China). The reference standards of fatty acid methyl esters were commercially obtained from Sigma-Aldrich. All other reagents such as glutaraldehyde (GA), ammonium iron (II) sulfate hexahydrate, methyl acrylate, ethylenediamine (EDA) and thionyl chloride were of analytical grade and purchased from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China).
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2

Antibacterial and Antioxidant Assays of Star Anise and Black Pepper

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Star anise fruits and black pepper fruits were bought from Jiangda South Road Vegetable Market, Nanchang City, China. Magnesium sulfate (MgSO4), potassium dichromate (K2Cr2O7), sulfonic type solid acid, potassium permanganate (KMnO4), hydrochloric acid (HCl), ethanol absolute, hydrogen peroxide solution (30 wt. % in H2O, H2O2), Iron(III) chloride (FeCl3), methanol, sodium carbonate (Na2CO3), hexane, formaldehyde, thionyl chloride (SOCl2), ethyl acetate, sodium hydroxide (NaOH), acetone, diethyl ether, potassium hydroxide (KOH), benzene, sodium bicarbonate (NaHCO3), N,N-Dimethylformamide (DMF), 1,1-diphenyl-2-picryhydrazyl (DPPH), and butylated hydroxytoluene (BHT) were obtained from Sinopharm Chemical Reagent Co., Ltd.
The microorganisms Staphylococcus aureus (ATCC 33591) (MRSA), Streptococcus pyogenes (ATCC 19615), Escherichia coli (ATCC 9637), Salmonella typhi (ATCC 14028) and Pseudomonas aeruginosa (ATCC 10145) were purchased from Jinuo Biomedical Technology Co., Ltd (Hangzhou, China).
MTT [3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyl tetrazolium bromide] and C6 cells were bought from Cell Bank of Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences (Shanghai, China).
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3

Fabrication of Multifunctional Polymer Composites

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2.1. Reagents 3-(Methacryloxyl) propyl trimethoxysilane (KH-570, 98%), ethylene glycol dimethacrylate (EGDMA, 98%), 2,2 0 -azobis (isobutyronitrile) (AIBN, 99%), tetracycline (TC, analytical standard), amoxicillin (AMO, Z98%), levofloxacin (LEV, 95%), doxorubicin hydrochloride (DOX, 98%), rhodamine B (RhB, Z99.0%), oxytetracycline (OTC, Z98%), and chlorotetracycline hydrochloride (CTC, analytical standard) were all purchased from Aladdin Reagent Co., Ltd. (Shanghai, China). N,N-Dimethylformamide (DMF, Z99.5%), methacrylic acid (MAA, Z99.0%), tetrahydrofuran (THF, Z99.8%), toluene (Z99.5%), ethylene glycol (Z99.0%), 4-carboxy-3-fluorophenylboronic acid (98%), thionyl chloride (Z99.0%), N-carboxybenzyloxy-1,2diaminoethane hydrochloride (98.0%), Na2CO3 (Z99.8%), trimethylammonium (99%), acrylic chloride (97%), halloysite nanotubes (HNTs, 99%), Fe (NO3)3•9H2O (Z98.5%) and acetone (Z99.0%) were obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Double-distilled ultrapure water was purified with a Purelab ultra (Organo, Tokyo, Japan).
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