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Anti mouse igg hrp conjugate

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Anti-mouse IgG-HRP conjugate is a secondary antibody that binds to mouse primary antibodies. It is conjugated with horseradish peroxidase (HRP) enzyme, which can catalyze a colorimetric reaction for detection purposes.

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Lab products found in correlation

Anti rabbit igg hrp conjugate, by Cell Signaling Technology (4 mentions) Pvdf membrane, by Merck Group (3 mentions) Anti adam10, by Abcam (2 mentions) Anti cd63, by BD (2 mentions) Alexa fluor 488 goat anti mouse, by Thermo Fisher Scientific (2 mentions) Alexa fluor 555 goat anti rabbit igg, by Thermo Fisher Scientific (2 mentions) Anti haptoglobin, by Calibre Scientific (2 mentions) Anti cd81, by BD (2 mentions) Las 1000, by Fujifilm (2 mentions) Bradford reagent, by Bio-Rad (2 mentions) Anti mouse igg hrp conjugate, by Merck Group (2 mentions) Mouse monoclonal anti ha antibody, by Merck Group (2 mentions) Anti phospho p44 42 mapk antibody, by Cell Signaling Technology (2 mentions) Ecl prime western blot detection kit, by GE Healthcare (2 mentions) Mouse monoclonal gfp antibody, by Santa Cruz Biotechnology (2 mentions) Anti gapdh, by Cell Signaling Technology (2 mentions) Laemmli sample buffer, by Bio-Rad (1 mentions) Criterion tris hcl gel, by Bio-Rad (1 mentions) Anti β actin, by Abcam (1 mentions) Anti mouse igg alexa fluor 594 conjugate, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

HRP anti-mouse (4 citations) IgG (Mouse) (3 citations)

7 protocols using anti mouse igg hrp conjugate

1

Western Blot Antibody Protocol

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Primary antibodies were used at 1–2 μg ml−1 for immunoblotting: anti-ADAM10 (mouse monoclonal, Abcam ab73402), anti-CD63 (mouse monoclonal, BD Biosciences 556,019), anti-CD81 (mouse monoclonal, BD Biosciences 555,675), anti-Haptoglobin (rabbit polyclonal, Biozol, GTX 112,962–25). The following secondary antibodies were used: Alexa Fluor 488 goat anti-mouse and Alexa Fluor 555 goat anti-rabbit IgG (both from Life Technologies) and anti-mouse IgG-HRP conjugate and anti-rabbit IgG-HRP conjugate (both from Cell Signaling).
Lee J.H., Eberhardt M., Blume K., Vera J, & Baur A.S. (2020). Evidence for liver and peripheral immune cells secreting tumor-suppressive extracellular vesicles in melanoma patients. EBioMedicine, 62, 103119.
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2

Western Blot Analysis of Cellular Proteins

Cited 1 time
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Total cellular protein extractions were carried out as described previously 74 (link), and quantified using Bradford reagent (Bio-Rad), according to manufacturer’s instructions. Fifty μg of protein from each sample were resolved in a 12% (w/v) SDS–PAGE and transferred to polyvinylidene difluoride (PVDF) membranes (Merck Millipore). GFP-tagged strains were detected using 1:5,000 dilution of the mouse monoclonal GFP antibody (Santa Cruz Biotechnology) and secondary antibody anti-mouse IgG HRP conjugate (Cell Signaling Technology), at 1:10,000 dilution. For the HA-tagged proteins detection, a mouse monoclonal anti-HA antibody (Sigma) was used at 1:5,000 dilution as a primary antibody, followed by the same anti-mouse IgG HRP conjugate as a secondary antibody. The phosphorylated fractions of the MAP kinase, MpkA, were examined using anti-phospho p44/42 MAPK antibody (Cell Signaling Technologies) following the manufacturer’s instructions using a 1:2,000 dilution. The primary antibody was detected using an HRP-conjugated secondary antibody raised in rabbit (Sigma). Chemoluminescent detection was achieved using an ECL Prime Western Blot detection kit (GE HealthCare). To detect these signals on blotted membranes, the ECL Prime Western Blotting Detection System (GE Helthcare, Little Chalfont, UK) and LAS1000 (FUJIFILM, Tokyo, Japan) were used.
de Castro P.A., Pinzan C.F., dos Reis T.F., Valero C., Van Rhijn N., Menegatti C., de Freitas Migliorini I.L., Bromley M., Trentin G., Almeida F., Fleming A.B., Traynor A.M., Sarikaya-Bayram Ö., Bayram O., Malavazi I., Ebel F., Barbosa J.C., Fill T., Pupo M.T, & Goldman G.H. (2023). Vacuoles and peroxisomes are involved in Aspergillus fumigatus gliotoxin production and self-protection. Research Square.
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3

Characterization of Rat Kidney Proteins

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Antibodies were from the following sources: anti-Asb3 (biorbyt), anti-Chac2 (Novus biologicals), anti-Pex11b (Novus biologicals), anti-Sp5 (Abcam), anti-Gapdh (Cell Signaling), anti-mouse IgG-HRP conjugate (Cell Signaling) and anti-rabbit IgG-HRP conjugate (Cell Signaling). Dahl S, Dahl R and SHR rat kidney Samples were homogenized in ice-cold RIPA lysis buffer with protease inhibitor cocktail (Pierce) and Proteins were quantitated using the Pierce BCA protein assay kit. In brief, forty microgram of proteins were boiled with Laemmli sample buffer (Bio-Rad) for 5 minutes at 95° C. Protein samples were resolved using 4–15% Criterion Tris-HCl Gel (Bio-Rad) at room temperature, and transferred on to PVDF membrane (Millipore), blocked with 5% fat free milk powder and incubated with a primary antibody overnight at 4°C, washed and incubated with a secondary antibody conjugated with horseradish peroxidase. Immunoreactivity was detected by autoradiography using the Super Signal West Pico Chemiluminescent Substrate (Pierce).
Gopalakrishnan K., Kumarasamy S., Mell B, & Joe B. (2014). GENOME-WIDE IDENTIFICATION OF LONG NON-CODING RNAS IN RAT MODELS OF CARDIOVASCULAR AND RENAL DISEASE. Hypertension, 65(1), 200-210.
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4

Western Blot Analysis of Protein Extracts

Cited 1 time
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Total cellular protein extractions were carried out as described71 (link), and quantified using Bradford reagent (Bio-Rad), according to manufacturer’s instructions. Fifty µg of protein from each sample were resolved in a 12% (w/v) SDS–PAGE and transferred to polyvinylidene difluoride (PVDF) membranes (Merck Millipore). GFP-tagged strains were detected using 1:5,000 dilution of the mouse monoclonal GFP antibody (Santa Cruz Biotechnology) and secondary antibody anti-mouse IgG HRP conjugate (Cell Signaling Technology), at 1:5,000 dilution. For the HA-tagged proteins detection, a mouse monoclonal anti-HA antibody (Sigma) was used at 1:5,000 dilution as a primary antibody, followed by the same anti-mouse IgG HRP conjugate as a secondary antibody. The phosphorylated fractions of the MAP kinase, MpkA, were examined using anti-phospho p44/42 MAPK antibody (Cell Signaling Technologies) following the manufacturer’s instructions using a 1:10,000 dilution. The primary antibody was detected using an HRP-conjugated secondary antibody raised in rabbit (Sigma). Chemoluminescent detection was achieved using an ECL Prime Western Blot detection kit (GE HealthCare). To detect these signals on blotted membranes, the ECL Prime Western Blotting Detection System (GE Helthcare, Little Chalfont, UK) and LAS1000 (FUJIFILM, Tokyo, Japan) were used.
Alves de Castro P., Figueiredo Pinzan C., dos Reis T.F., Valero C., Van Rhijn N., Menegatti C., de Freitas Migliorini I.L., Bromley M., Fleming A.B., Traynor A.M., Sarikaya-Bayram Ö., Bayram Ö., Malavazi I., Ebel F., Barbosa J.C., Fill T., Pupo M.T, & Goldman G.H. (2024). Aspergillus fumigatus mitogen-activated protein kinase MpkA is involved in gliotoxin production and self-protection. Nature Communications, 15, 33.
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5

Antibody characterization in DNA damage response

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Anti-RTEL1, produced in Rabbit (Sigma, Cat. No. HPA067329); Anti-RPA1, produced in Mouse (Sigma, Cat. No. SAB1406399); Anti-RPA 32 kDa subunit (9H8), produced in Mouse (Santa Cruz Biotechnology, Cat. No. sc-56770); Anti-HA tag (C29F4), produced in Rabbit (Cell Signalling Technology, Cat. No. 3724); Anti-gamma H2A.X (phospho S139) antibody [3F2], produced in mouse (Abcam, Cat. No. ab22551); Anti-beta Actin, produced in Mouse (Abcam, Cat. No. ab8226); Anti-Rabbit IgG, HRP-conjugate (Millipore, Cat. No. 12–348); Anti-Mouse IgG, HRP-conjugate (Cell Signalling Technology, Cat. No. 7076); Anti-Rabbit IgG, Alexa Fluor™ 488-conjugate (Invitrogen, Cat. No. A-11008); Anti-Rabbit IgG, Alexa Fluor™ 594-conjugate (Invitrogen, Cat. No. SA5-10040); Anti-Mouse IgG, Alexa Fluor™ 488-conjugate (Invitrogen, Cat. No. A-11001); Anti-Mouse IgG, Alexa Fluor™ 594-conjugate (Invitrogen, Cat. No. A-11032)
Kumar N., Taneja A., Ghosh M., Rothweiler U., Sundaresan N.R, & Singh M. (2023). Harmonin homology domain-mediated interaction of RTEL1 helicase with RPA and DNA provides insights into its recruitment to DNA repair sites. Nucleic Acids Research, 52(3), 1450-1470.
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6

Immunodetection of Extracellular Vesicle Markers

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Primary antibodies were used at 1–2 μg·ml−1 for immunoblotting, 2 μg·ml−1 for immunofluorescence, and 1–10 μg·ml−1 for MELC. The following antibodies were used for immunostaining, flow cytometry, or immunoblotting: anti-ADAM10 (mouse monoclonal, ab73402; Abcam), anti-CD63 (mouse monoclonal, 556019; BD Biosciences), anti-CD81 (mouse monoclonal, 555675; BD Biosciences), anti-haptoglobin (rabbit polyclonal, GTX 112962-25; Biozol), and anti-trimethyl histone H3 Lys27 (rabbit monoclonal, #9733; Cell Signalling). The following secondary antibodies were used: Alexa Fluor 488 goat anti-mouse and Alexa Fluor 555 goat anti-rabbit IgG (both from Life Technologies) and anti-mouse IgG-HRP conjugate and anti-rabbit IgG-HRP conjugate (both from Cell Signalling).
Lee J.H., Dindorf J., Eberhardt M., Lai X., Ostalecki C., Koliha N., Gross S., Blume K., Bruns H., Wild S., Schuler G., Vera J, & Baur A.S. (2019). Innate extracellular vesicles from melanoma patients suppress β-catenin in tumor cells by miRNA-34a. Life Science Alliance, 2(2), e201800205.
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7

Western Blot Analysis of Cellular Signaling Pathways

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Cell lysates were separated by SDS-PAGE (Bio-Rad; 4561036). Anti-IFT20 (Proteintech; 13615-AP, 1:1,000), anti-GAPDH (Cell Signaling technology; #2118, 1:5,000), anti-GLI1 (R&D systems; AF3455, 1:500), anti-SOX9 (Santa cruz biotechnology; sc-20095, 1:1,000), anti-phospho ERK1/2 (Cell Signaling technology; #4376, 1:1,000), anti-ERK1/2 (Cell Signaling technology; #4695, 1:1,000), anti-active (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
β-Catenin (Cell signaling technology; #8814, 1:1,000), anti-β-Catenin (BD Biosciences; #610153, 1:1,000), anti-rabbit IgG HRP-conjugate (Cell Signaling technology; #7074, 1:5,000), anti-mouse IgG HRP-conjugate (Cell Signaling technology; #7076, 1:5,000) and anti-goat IgG HRP-conjugate (Invitrogen; #PA1-28664, 1:2,000) antibodies were used for western blotting. The Clarity Max ECL Substrate (Bio-Rad; 1705061) was used for chemiluminescent detection on a Chemidoc XRS+ imaging system (Bio-Rad), and the signals were quantified using the Image Lab Version 6.0 software (Bio-Rad) and the image-J software.
Yamaguchi H., Kitami M., Koecklin K.H.U., Li H., Wang J., Perrien D.S., Lagor W.R., & Komatsu Y. (2021). IFT20 is critical for early chondrogenesis during endochondral ossification.
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