The following materials were used in the current study: blood glucose meter (Roche Diagnostic, Basel, Switzerland), fluorescence b spectrophotometer (Hitachi, Tokyo, Japan), streptozotocin (Sigma Chemical Co., St. Louis, MO, USA), rat insulin enzyme-linked immunosorbent assay (ELISA) kit (Crystal Chem. Inc., USA), AGEs Kit (R&D Systems. Inc., USA), rat RAGE ELISA Kit (R&D Systems. Inc., USA). Apomorphine (APO) (Sigma Chemical Co.). Microtubule-associated protein 1 light chain 3 (LC3), Beclin1, P70S6K, PI3K, AKT, mammalian target of rapamycin (mTOR), phospho-AKT, phospho-mTOR, and phospho-P70S6K antibodies (Cell Signaling Technologies, USA). Type II collagenase kit (Biosharp, USA). Dulbecco’s modified Eagles medium (Thermo Fisher Scientific, USA), fetal bovine serum (Thermo Fisher Scientific, USA), M100, and NIBP multi-electric physiography (Biopac System, USA).
Apomorphine apo
Manufactured by Merck Group
Sourced in China
Apomorphine (APO) is a laboratory-grade compound commonly used in scientific research. It is a dopamine agonist that primarily functions by directly stimulating dopamine receptors. The core function of Apomorphine is to serve as a valuable tool for researchers investigating dopaminergic systems and related physiological and neurological processes.
Automatically generated - may contain errors
Lab products found in correlation
Streptozotocin (stz), by Merck Group (2 mentions)
6 ohda, by Merck Group (2 mentions)
Ascorbic acid, by Merck Group (2 mentions)
Blood glucose meter, by Roche (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
Ages kit, by R&D Systems (1 mentions)
Streptozotocin (stz), by Solarbio (1 mentions)
Sybr green supermix, by Bio-Rad (1 mentions)
Rabbit anti th antibody, by Abcam (1 mentions)
Anti gfap antibody, by Abcam (1 mentions)
Rabbit anti iba1 antibody, by Abcam (1 mentions)
Vitamin c, by Merck Group (1 mentions)
Cd105, by BD (1 mentions)
D amphetamine sulfate amph, by Merck Group (1 mentions)
Ascorbic acid saline, by Merck Group (1 mentions)
7 protocols using apomorphine apo
1
Investigating Diabetic Complications and Therapeutic Interventions
2
Streptozotocin-Induced Diabetes Model
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Streptozotocin (STZ) was acquired from Solarbio life science (Beijing, China), apomorphine (APO) and TM were purchased from Sigma-Aldrich (St Louis, MO).
3
Neuroinflammation and Parkinson's Disease
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6-OHDA, apomorphine (APO), and ascorbic acid were purchased from Sigma Aldrich (St. Louis, MO, USA). INF-γ was bought from Sino Biological Inc. (Beijing, China). Fetal bovine serum (FBS) and Roswell Park Memorial Institute (RPMI) 1640 medium were obtained from HyClone (Logan, UT, USA) and Invitrogen (Carlsbad, CA, USA), respectively. Rabbit anti-TH antibody, rabbit anti-Iba1 antibody, and anti-GFAP antibody were obtained from Abcam (Cambridge, UK). Immunohistochemical kit (containing hydrogen peroxide, blocking solution, horseradish peroxidase- (HRP-) conjugated secondary antibody, and 3,3-diaminobenzidine (DAB)) was purchased from UNIV (Shanghai, China). SYBR Green Supermix was obtained from Bio-Rad (USA).
4
Rat Model for Diabetes-Induced Endothelial Dysfunction
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CD34, CD45, CD73, CD90 and CD105 antibodies were purchased from BD Biosciences. Streptozotocin, apomorphine (APO) and vitamin C were purchased from Sigma-Aldrich; Merck KGaA. Anti-eNOS and β-actin antibodies were purchased from Cell Signaling Technology, Inc. Modified Lillie-Mayer hematoxylin staining solution, ethanol eosin staining solution and Sirius Red staining solution were purchased from Reagan.
Male Sprague-Dawley (SD) rats were provided by Guangdong Pharmaceutical University. All animal experiments were approved by the Animal Ethics Committee of Guangdong Pharmaceutical University.
Male Sprague-Dawley (SD) rats were provided by Guangdong Pharmaceutical University. All animal experiments were approved by the Animal Ethics Committee of Guangdong Pharmaceutical University.
5
Poly(I:C)-Induced Maternal Immune Activation
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High molecular weight (HMV) poly (I:C) was purchased from InvivoGen. Poly (I:C) was dissolved in endotoxin-free saline solution, (4.0 mg/kg) and injected in the lateral vein of the tail of F0 pregnant dams. To assess the efficacy of poly (I:C) injection, all pregnant rats were weighed for the first 3 days after the administration of either poly (I:C) or endotoxin-free saline to evaluate potential weight loss as underlined by previous investigations (Zuckerman et al., 2003 (link)).
Apomorphine (APO, 0.125 mg/kg) (Merck Sigma-Aldrich) was dissolved in a solution containing 0.9% NaCl with 0.1 mg/ml ascorbic acid (pH 7.2) to prevent oxidation. D-Amphetamine sulfate (AMPH, 0.1 mg/kg) (Merck Sigma-Aldrich) was dissolved in a solution containing 0.9% NaCl. APO and AMPH were administered subcutaneously and intraperitoneally in an injection volume of 1 and 2 ml/kg, respectively.
Apomorphine (APO, 0.125 mg/kg) (Merck Sigma-Aldrich) was dissolved in a solution containing 0.9% NaCl with 0.1 mg/ml ascorbic acid (pH 7.2) to prevent oxidation. D-Amphetamine sulfate (AMPH, 0.1 mg/kg) (Merck Sigma-Aldrich) was dissolved in a solution containing 0.9% NaCl. APO and AMPH were administered subcutaneously and intraperitoneally in an injection volume of 1 and 2 ml/kg, respectively.
6
Preparation of Psychotropic Compounds
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Olanzapine API (purity > 99%) was obtained from Shanghai Aladdin Bio-Chem Technology Co., Ltd. (Shanghai, China) and dissolved in a minimum volume of glacial acetic acid before adding purified water, and pH was adjusted to 6.0 with 0.1 M NaOH. SEP-363856 API (purity > 95%) was synthesized in our laboratory, and dissolved in purified water. Apomorphine (APO) was obtained from Sigma-Aldrich (Shanghai, China) Trading Co., Ltd., and dissolved in saline (with 0.1% ascorbic acid). Dizocilpine maleate (MK-801) was obtained from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China) and dissolved in saline before use.
7
Rat Model of Parkinson's Disease
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Adaptively raised for 1 week, SD rats were randomly divided into two groups: the sham‐operated group (n = 6) and the 6‐OHDA‐lesioned group (n = 36). After deep anesthesia, the rats were stereotaxically injected with 3 μL 6‐OHDA (4 mg/mL in 0.02% ascorbic acid saline; Sigma) into the left striatum following our previously described protocol,
17 and the rats in sham group were injected with 3 μL vehicle (0.02% ascorbic acid saline).
The damage of rat dopaminergic neurons was assessed by apomorphine (APO, Sigma)‐induced rotation tests at the 5th, 8th, and 11th weeks of experiment.
17 At the end of the test on the 5th week, the 6‐OHDA‐lesioned rats were randomly divided into five treatment groups: (1) 6‐OHDA + L‐dopa (60 mg/kg) group,
20 (link) (2) 6‐OHDA + PIP (10 mg/kg) group, (3) 6‐OHDA + PIP (20 mg/kg) group, (4) 6‐OHDA + L‐dopa (60 mg/kg) + PIP (20 mg/kg) group, and (5) 6‐OHDA + madopar (75 mg/kg) group. The dosages of PIP used in the present study were safe for rats.
21 (link) Rats were treated once a day for 6 weeks. The 6‐OHDA group and sham group were treated with the same volume of substrate solution (0.5% CMC‐Na).
17 and the rats in sham group were injected with 3 μL vehicle (0.02% ascorbic acid saline).
The damage of rat dopaminergic neurons was assessed by apomorphine (APO, Sigma)‐induced rotation tests at the 5th, 8th, and 11th weeks of experiment.
17 At the end of the test on the 5th week, the 6‐OHDA‐lesioned rats were randomly divided into five treatment groups: (1) 6‐OHDA + L‐dopa (60 mg/kg) group,
20 (link) (2) 6‐OHDA + PIP (10 mg/kg) group, (3) 6‐OHDA + PIP (20 mg/kg) group, (4) 6‐OHDA + L‐dopa (60 mg/kg) + PIP (20 mg/kg) group, and (5) 6‐OHDA + madopar (75 mg/kg) group. The dosages of PIP used in the present study were safe for rats.
21 (link) Rats were treated once a day for 6 weeks. The 6‐OHDA group and sham group were treated with the same volume of substrate solution (0.5% CMC‐Na).
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