Zen 2012 sp1 software black edition

The confocal analysis by means of a Zeiss Axio Observer Z1 (Carl Zeiss Microscopy GmbH, Köln, Germany) confocal inverted microscope (LSM 710 model) was used to observe the features of MBC. The microscope was equipped with the following laser scanning systems: diode laser (405 nm), Ar laser (458, 488, and 514 nm), DPSS (561 nm pumped solid-state diodes), and HeNe laser (633 nm). The distribution of the pigments into the complex matrix was observed using a 20× apochromatic objective and two types of magnification, 0.6 and 1. MBC was analyzed in its native state and also fluorescently labeled with two dyes, DAPI (1 µg/mL) and Red Congo (40 µM), in a ratio of 3:1:1. Furthermore, the comparative confocal microscopy analysis was also carried out with the experimental pastry creams in order to capture the structural, textural, and compositional changes. The pastry creams variants were observed in their native state and stained with the two fluorophores dyes, and the obtained 3D images were rendered and analyzed with the ZEN 2012 SP1 software (Black Edition, Carl Zeiss Microscopy GmbH, Jena, Germany).

In order to observe the structure and the morphology of the sea buckthorn encapsulated powders, a confocal analysis was performed on a Zeiss Axio Observer Z1 (Carl Zeiss Microscopy GmbH, Köln, Germany) confocal inverted microscope. The LSM 710 microscope consists of several laser scanning systems: diode laser (405 nm), Ar laser (458, 488, and 514 nm), DPSS (561 nm pumped solid-state diodes), and HeNe laser (633 nm). The distribution of the biologically active compounds from sea buckthorn into the complex matrix was observed using a 20× apochromatic objective and the 0.6 magnification. Both of the obtained powders were analyzed in their native state and also fluorescently labeled with 4′,6-diamidino-2-phenylindole (DAPI) (1 µg/mL) and Red Congo (40 µM), in a ratio of 3:1:1. The captured confocal images of the encapsulated powders were analyzed with the ZEN 2012 SP1 software (Black Edition, Carl Zeiss Microscopy GmbH, Jena, Germany).

The structure and the morphology of the obtained powders were determined using a confocal analysis performed on a Zeiss Axio Z1 Observer confocal inverted microscope (Carl Zeiss Microscopy GmbH, Köln, Germany). The LSM 710 microscope uses several laser scanning systems: Diode laser (405 nm), Ar laser (458, 488, and 514 nm), DPSS (561 nm pumped solid-state diodes), and HeNe laser (633 nm). The distribution of bioactives into the complex biopolymer matrix was observed using the 20× apochromatic objective and the 0.6 magnification. The obtained powders were observed both in their native state and fluorescently labeled with Red Congo (40 µM), in a ratio of 3:1. The confocal images of the powders were captured and analyzed with the ZEN 2012 SP1 software (Black Edition, Carl Zeiss Microscopy GmbH, Jena, Germany).