The specific RORC inhibitor SR221136 (link) was purchased commercially (Tocris/Biotechne) and resuspended in DMSO at a stock of 100 mM. SR2211 was added at a final concentration of 10 μM in both bulk and single cell cultures; controls were treated with the equivalent concentration of DMSO (0.01%). SR2211 toxicity was tested on growing ILC cultures using a range of 5–100 μM; no effect was seen at 10 μM which was the dose previously reported36 (link). SR2211 and control DMSO were replenished every 2–3 days for bulk cultures and every 4–5 days for single cell cloning.
Sr2211
Manufactured by Bio-Techne
Sourced in United States, United Kingdom
SR2211 is a laboratory instrument designed for the detection and quantification of proteins. It utilizes a specific technology to enable accurate and reliable protein analysis. The core function of SR2211 is to provide researchers with a tool for measuring and analyzing protein samples in a controlled and efficient manner.
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Lab products found in correlation
4 protocols using sr2211
1
RORC Inhibitor SR2211 in ILC Culture
2
Synthesis and Purification of Compounds
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SR2211 was purchased from TOCRIS (Minneapolis, MN, USA cat. no. 4869). XY018, Cmpd 31 and GSK805 were synthesized and purified to >98–99% purity by WuXi AppTec. PBK/TOPK inhibitor HI-TOPK-032 was from Sigma (St. Louis, MO, USA cat. no. SML0796). Other chemicals were purchased from Thermo Fisher Scientific.
3
Synthesis and Sourcing of Compounds
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4
Synthesis of Imidazo[1,2-a]pyridine-Based RORγt Inhibitor
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Cpd A, [(S)-N-(8-((4-(5-(tert-butyl)-1,2,4-oxadiazol-3-yl)-3-methylpiperazin-1-yl)methyl)-7-methylimidazo[1,2-a]pyridin-6-yl)-6-methylnicotinamide] was synthesized as described in Figure S1 . Briefly, the 2-methylpyrimidine-5-carboxamide group of (S)-N-(8-((4-(5-(tert-butyl)-1,2,4-oxadiazol-3-yl)-3-methylpiperazin-1-yl)methyl)-7-methylimidazo[1,2-a]pyridin-6-yl)-2-methylpyrimidine-5-carboxamide [prepared as described in Hintermann et al. (34 (link))] was cleaved with sodium hydroxide. The intermediate amine was subsequently converted into Cpd A by reaction with 6-methylnicotinic acid using T3P as the amide-coupling reagent.
The published RORγt inhibitor SR2211 (42 (link)) and the pan-JAK inhibitor CP690550 (43 (link)) were purchased from Tocris.
The published RORγt inhibitor SR2211 (42 (link)) and the pan-JAK inhibitor CP690550 (43 (link)) were purchased from Tocris.
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