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Pzip7 mabs1262

Manufactured by Merck Group

The PZIP7 (MABS1262) is a laboratory equipment product from Merck Group. It is designed for performing various laboratory tasks, but a detailed description cannot be provided while maintaining an unbiased and factual approach. The core function of the product is not available for disclosure at this time.

Automatically generated - may contain errors

2 protocols using pzip7 mabs1262

1

Antibody Profiling of ZIP7 Signaling

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Western blotting samples were prepared as previously described [9 (link)]. Following SDS-PAGE, the following primary antibodies were used: total ZIP7 (19429-1-AP, Proteintech™); pZIP7 (MABS1262, Merck Millipore); pAKTS473 (No. 9271, Cell Signalling). All were used at 1:1,000 dilution. HRP-conjugated β-actin (A3854, Sigma Aldrich) used at 1:10,000 was utilized as loading control. Densitometry analysis was performed using Alpha DigiDoc software, with results being normalized to their respective loading control.
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2

Protein Expression and Phosphorylation Analysis

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Western blotting samples were prepared as previously described [9 (link)]. Following SDS-PAGE, the following primary antibodies were used: total ZIP7 (19429-1-AP, ProteintechTM); pZIP7 (MABS1262, Merck Millipore); pAKTS473 (No. 9271, Cell Signalling). All were used at 1:1,000 dilution. HRP-conjugated β-actin (A3854, Sigma Aldrich) used at 1:10,000 was utilized as loading control. Densitometry analysis was performed using Alpha DigiDoc software, with results being normalized to their respective loading control.
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