The generation of conditional Idh1-R132Q-LSL mice was as described. Idh1-R132Q-LSL mice (Sasaki et al., 2012b (link)) were bred with Vav-Cre mice (Jackson Laboratories; Cat.No. 008610) to produce Vav-IDH1-KI mice (C57BL6/129Ola9; F10). Tet2 KO mice were from Jackson Laboratories (Cat.No: 023359) (Ko et al., 2011 (link)) or as described (Moran-Crusio et al., 2011 (link)). Atm KO and Tet1 KO mice were as described (Cimmino et al., 2015 (link); Ito et al., 2004 (link)). CD45.1 mice were from Jackson Laboratories (Cat.No: 002014). The generation of conditional Idh1-R132H–LSL mice was as described (Hirata et al., 2015 (link)). Cre-ERT;Idh1-R132H-LSL mice used for primary MEF generation were produced by crossing Idh1-R132H-LSL mice with CreERT mice (Jackson Laboratories; Cat.No. 004847). For all experiments, young mice were analyzed at 3–4 months, and aged mice were analyzed at 7–10 months. All animal experiments were approved by the University Health Network Animal Care Committee (UHN-ACC) (ID: AUP985). Primers for genotyping PCR were: Idh1 (5’-ACC AGCACCTCCCAACTTGTAT-3’, 5’-AGGTTAGCTCTTGCCGATCCGT-3’, 5’-CAGCAGCCTCTGTTCCACATAC-3’); Vav-Cre (5’-AGATGCCAGGACATCAGGAACCTG-3’, 5’-ATCAGCCACACCAGACACAGAGATC-3’); and CreERT (5’-GCGGTCTGGCAGTAAAACTATC-3’, 5’-CTGAAACAGCATTGCTGTCACTT-3’).
Tet2 ko mice
Manufactured by Jackson ImmunoResearch
Sourced in United States
Tet2 KO mice are genetically modified mice with a targeted deletion of the Tet2 gene. The Tet2 gene is involved in the regulation of DNA methylation, a epigenetic process that plays a role in gene expression. These mice can be used as a model to study the biological functions of the Tet2 gene and its involvement in various cellular and physiological processes.
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2 protocols using tet2 ko mice
1
Generation of Conditional IDH1 Mutant Mice
2
Xenograft Tumor Model in Mice
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Tet2 KO mice were purchased from The Jackson Laboratory (USA). For transplantation of HepG2 cells or MHCC97H cells to BALB/c nude mice, cells were concentrated to 106 cells per 100 μL PBS, which were then mixed with equal volumes of Matrigel (Corning). Overall, 200 μL cell mixtures were subcutaneously injected into the flank of 6-week-old male BALB/c nude mice. For rapamycin treatment, rapamycin in ethanol at 10 mg/mL was diluted in 5% Tween-80 and 5% PEG-400 (Meilun Biotechnology, China). Treatment was conducted by intraperitoneal injection of 3 mg/kg every other day starting at day 4 (the day of transplantation). Tumor volume was calculated as volume = width2 × length × 0.5. All animal experiments were approved by the ethic committee of School of Basic Medical Sciences, Fudan University. All animals used in this study received appropriate care according to institutional guidelines.
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