Acetone

Manufactured by EKF Diagnostics

Sourced in Poland

Acetone is a laboratory reagent used to facilitate various chemical processes and analyses. It serves as a solvent, cleaning agent, and extraction medium in various laboratory applications.

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7 protocols using acetone

Bioactive Compound Analysis Protocol

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The following chemicals were used for analysis of the content of bioactive compounds: sodium bicarbonate (Honeywell), potassium sodium tartrate tetrahydrate (Merck), potassium hydroxide (Merck), phenolphthalein (Avantor), Sudan III (Waldeck), sodium hydroxide (Avantor), copper(II) sulphate (Avantor), mercuric chloride (Avantor), mercuric nitrate (Sigma Aldrich), mercurous nitrate (Alfa Aesar), nitric acid (Merck), sulphuric acid (Avantor), ammonium hydroxide (Supelco), ninhydrin (Sigma Aldrich), acetone (Stanlab), chloroform (Avantor), acetic acid (Supelco), acetic anhydride (Avantor), picric acid (Sigma Aldrich), tannic acid (Alfa Aesar), mercury(II) chloride (Avantor), bismuth subnitrate (Honeywell), glacial acetic acid (Supelco), potassium iodide (Sigma Aldrich), hydrochloric acid (Avantor), iodine (Sulpeco), multi-elemental stock ICP standard solution (no. XVI, Merck), single ICP stocks of As, Hg, P, S, and Se (Merck).

Godlewska K., Pacyga P., Szumny A., Szymczycha-Madeja A., Wełna M, & Michalak I. (2022). Methods for Rapid Screening of Biologically Active Compounds Present in Plant-Based Extracts. Molecules, 27(20), 7094.

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Synthesis and Characterization of Furan-Based Monomers

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All reagents were commercially available materials and were used without further purification. Maleic anhydride (≥98%) and dibutyltin dilaurate (95%) were provided by Alfa Aesar (Haverhill, MA, USA). Furan (≥99%) was obtained from Fluka. Hydroquinone (≥99%), 2-hydroxypropyl acrylate (HPA) (mixture of the isomers, 95%), 2-hydroxyethyl methacrylate (HEMA) (97%), hexamethylene diisocyanate (for synthesis), polyethylene glycol PEG 1000 (for synthesis), and 2-hydroxypropyl methacrylate (HPMA) (mixture of hydroxypropyl and 2-hydroxypropyl methacrylates, ≥97%) were purchased from Sigma-Aldrich (Steinheim am Albuch, Germany). Furfuryl alcohol (98%) and isophorone diisocyanate (98%) were provided by Acros Organics (Geel, Belgium). 2-hydroxyethyl acrylate (HEA) was kindly donated by Cognis Performance Chemicals (Hythe, UK). Chempur (Piekary Śląskie, Poland) provided analytical-grade ethanolamine and triethanolamine. Diethyl ether (99.5%) and anhydrous ethanol p.a. (99.8%) were provided by Avantor Performance Materials Poland S.A. (POCH, Gliwice, Poland). Acetone, isopropanol, xylene, toluene, and dichloromethane of high purity were purchased from StanLab (Lublin, Poland).

Bednarczyk P., Mozelewska K., Klebeko J., Rokicka J, & Ossowicz-Rupniewska P. (2023). Impact of the Chemical Structure of Photoreactive Urethane (Meth)Acrylates with Various (Meth)Acrylate Groups and Built-In Diels–Alder Reaction Adducts on the UV-Curing Process and Self-Healing Properties. Polymers, 15(4), 924.

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Polylactide and PEG Synthesis

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Acetone and chloroform were purchased from STANLAB (Lublin, Poland). Polylactide (type 2002D, average molecular weight = 79 kDa) was delivered in the form of pellets by Nature Works^® (Minnetonka, MN, USA). Poly(ethylene glycol) (M_w = 1500 g·mol⁻¹) was purchased from Sigma-Aldrich (Steinheim, Germany).

Grabska-Zielińska S., Gierszewska M., Olewnik-Kruszkowska E, & Bouaziz M. (2021). Polylactide Films with the Addition of Olive Leaf Extract—Physico-Chemical Characterization. Materials, 14(24), 7623.

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Antioxidant Potential of Plant Extracts

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All chemicals and reagents used in this study were of analytical or HPLC grade. 2,2-Diphenyl-1-picryhydrazyl hydrate stable radical (DPPH^•, 95%), 2,2-azino-bis(3-ethyl- benzothiazoline-6-sulfonic acid) diammonium salt (ABTS, 98%), 2.0 M Folin–Ciocalteu phenol reagent, KCl, Na₂HPO₄, K₂S₂O₈, NaCl, Na₂CO₃, and HPLC grade acetonitrile were purchased from Merck (Darmstadt, Germany); KH₂PO₄ from Jansen Chimica (Beerse, Belgium); 2,4,6-tripyridyl-s-triazine (TPTZ) from Fluka Chemicals (Steinheim, Switzerland). Acetone, methanol (MeOH), n-hexane and acetic acid were obtained from StanLab (Lublin, Poland); agricultural origin ethanol (96.6%) from Stumbras (Kaunas, Lithuania). Dimethyl sulfoxide (DMSO), n-butanol and ethyl acetate were purchased from Sigma-Aldrich (Saint-Quentin-Fallavier, France). Reference compounds, mangiferin and hydroxytormentic acid were purchased from Sigma Aldrich (Steinheim, Germany), and tormentic acid from ChemFaces (Wuhan, Hubei, China).

Grauzdytė D., Pukalskas A., El Kalamouni C, & Venskutonis P.R. (2020). Mangiferin Rich Products from Aphloia theiformis (Vahl) Benn Leaves: Extraction, Fractionation, Phytochemical Characterization, and Antioxidant Properties. Molecules, 25(9), 2081.

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Electrochemical Characterization of Ti-Mn Alloys

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Acetone, isopropanol, and methanol were purchased from P.P.H. “STANLAB” Sp. J. (Lublin, Poland), while ethylene glycol (EG) from CHEMPUR and ammonium fluoride from ACROS ORGANICS. Technical grade Ti foils and Ti–Mn alloys with 5, 10 and 15 wt. % of manganese content were provided by HMW-Hauner Metallische Werkstoffe (Röttenbach, Germany). Deionized (DI) water with conductivity of 0.05 μS was used to prepare all aqueous solutions.

Nevárez-Martínez M.C., Kobylański M.P., Mazierski P., Wółkiewicz J., Trykowski G., Malankowska A., Kozak M., Espinoza-Montero P.J, & Zaleska-Medynska A. (2017). Self-Organized TiO2–MnO2 Nanotube Arrays for Efficient Photocatalytic Degradation of Toluene. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 22(4), 564.

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Carotenoid and Isoprenoid Quinone Extraction

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Acetone and methanol used for the extraction of carotenoids and isoprenoid quinones were purchased from Stanlab (Lublin, Poland). HPLC grade methanol and methyl tert-butyl ether (MTBE) were purchased from J.T. Baker (Deventer, The Netherlands). Ultra-pure water was obtained from a Milli-Q water purification system (Millipore Corp., Bedford, MA, USA). Chromacol PTFE syringe filters (0.2 μm pore size) were purchased from Shim-Pol (Izabelin, Poland).

Antolak H., Oracz J., Otlewska A., Żyżelewicz D, & Kręgiel D. (2017). Identification of Carotenoids and Isoprenoid Quinones from Asaia lannensis and Asaia bogorensis. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 22(10), 1608.

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Solvent Preparation for Analytical Assays

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Gallic acid, 96.6% ethanol, 2-propanol, disodium hydrogen phosphate, dihydrogen sodium phosphate (Chempur, Piekary Śląskie, Poland), HCl, NaOH (POCh, Gliwice, Poland), acetone, n-hexane, methanol, DPPH -2,2-diphenyl-1-picrylhydrazyl, acetonitrile (Sigma-Aldrich, St. Louis, MO, USA), acetone (StanLab, Lublin, Poland) were of analytical-reagent grade and were used without further purification. Water was three times distilled in a quartz apparatus. Solvents were filtered through a (0.22 μm) membrane filter (Millipore, Bedford, USA).

Piszcz P., Tomaszewska M., & Głód B.K. (2020). Estimation of the total antioxidant potential in the meat samples using thin-layer chromatography. Open Chemistry, 18(1), 50-57.

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