The complete genome was sequenced using 454 technology with the GS Junior 454 system platform (Roche Applied Science, Indianapolis, IN, USA), yielding 6.5-fold coverage of the phage genome [68 (link)]. Prediction of open reading frames (ORFs) was performed using softberry (http://linux1.softberry.com/ (accessed on 8 February 2021)) and Glimmer. A whole-genome sequence assembly was performed using Geneious software version 6 while Phage termini were identified using the method described by Zhang et al. [69 (link)].
Gs junior 454 system platform
Manufactured by Roche
Sourced in United States
The GS Junior 454 system platform is a compact sequencing instrument designed for targeted and small-scale genomic applications. It utilizes 454 sequencing technology to generate high-quality, long sequence reads. The system is capable of performing next-generation DNA sequencing and is suitable for a variety of research applications.
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Lab products found in correlation
2 protocols using gs junior 454 system platform
1
Genome Sequencing and Assembly of Phage
2
Complete Genome Sequencing of Mycobacteriophage
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The complete genome was sequenced, using 454 technology, with the GS Junior 454 system platform (Roche Diagnostics, Indianapolis, IN, USA), yielding 145-fold coverage of the phage genome. The 15 available nucleotide sequences of the mycobacteriophage genomes in the F cluster were retrieved from the NCBI nucleotide database. Based on the phage coding sequence, a phylogenetic tree was drawn, using the neighbor linkage (NJ) method. The bootstrap value was 1000 replicates on MEGA X [19 (link)].
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