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Fitc conjugated anti cd42b antibody

Manufactured by Abcam
Sourced in United States

FITC-conjugated anti-CD42b antibody is a laboratory reagent used to detect and analyze the presence of CD42b, also known as the platelet glycoprotein Ib alpha chain, on the surface of cells. The antibody is conjugated with the fluorescent dye FITC, which allows for visualization and quantification of CD42b-expressing cells using techniques such as flow cytometry.

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4 protocols using fitc conjugated anti cd42b antibody

1

Salidroside Modulates Platelet Activation

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Salidroside was purchased from MedChemExpress (Monmouth Junction, NJ, USA) with a purity ≥ 98%. Collagen-related peptide (CRP) was prepared as previously described [56 (link)]. Thrombin (≥ 10 NIH units/vial) were from Chrono-log Corporation (Havertown, PA, USA). FITC-conjugated mouse anti-human CD41a and PAC-1 antibody were from BD Biosciences (San Jose, CA, USA) and BECTON DICKINSON (San Jose, CA, USA) respectively. PE-conjugated anti-human/mouse CD62p (P-Selectin) and anti-human Glycoprotein VI purified antibody were purchased from eBioscience (San Diego, CA, USA). FITC-conjugated anti-CD42b antibody was from Abcam (Cambridge, MA, USA). FITC-conjugated goat anti-mouse IgG was purchased from ZSGB-BIO (Beijing, China). β-actin antibody and anti-rabbit IgG (HRP-linked) antibody were purchased from Cell Signaling Technology (Danvers, MA, USA). SB216763 (GSK-3β inhibitor) was purchased from ApexBio Technology.
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2

Platelet Activation and Aggregation Assay

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Thalidomide was purchased from Changzhou Pharmaceutical Factory Co., Ltd (Changzhou, Jiangsu, China). Collagen and ADP were from Helena laboratories (Beaumont, Texas, USA). Collagen-related peptide (CRP) was prepared as previously described [21 (link)]. FITC-conjugated anti-human CD41a and PAC-1 antibody were from BD Biosciences (San Jose, CA) and Becton Dickinson (San Jose, CA) respectively. PE-conjugated anti-human/mouse CD62p (P-Selectin) and purified anti-human Glycoprotein (GP) VI antibody were purchased from eBioscience (San Diego, CA). FITC-conjugated anti-CD42b antibody was from Abcam (Cambridge, MA). FITC-conjugated goat anti-mouse IgG was from ZSGB-BIO (Beijing, China). Oregon Green-labelled fibrinogen (OG-Fng) was from Thermo Fisher Scientific (Waltham, MA).
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3

Platelet Aggregation Inhibition by Platycodin D

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Platycodin D (PD) was purchased from Fluka (Steinheim, Germany) with a purity ≥ 99%. Collagen, adenosine diphosphate (ADP), arachidonic acid and epinephrine were from Helena laboratories (Beaumont, Texas, USA). Thrombin was purchased from Sigma-Aldrich (St. Louis, MO, USA). FITC-conjugated mouse anti-human CD41a and PAC-1 antibody were from BD Biosciences (San Jose, CA, USA) and Becton–Dickinson (San Jose, CA, USA) respectively. PE-conjugated anti-human/mouse CD62p (P-Selectin) and anti-human Glycoprotein VI purified antibody were purchased from eBioscience (San Diego, CA, USA). FITC-conjugated anti-CD42b antibody was from Abcam (Cambridge, MA, USA). FITC-conjugated goat anti-mouse IgG was purchased from ZSGB-BIO (Beijing, China). β-actin antibody and anti-rabbit IgG (HRP-linked) antibody were purchased from Cell Signaling Technology (Danvers, MA, USA).
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4

Platelet Activation Assay Using Flow Cytometry

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ATRA was purchased from Sigma-Aldrich (St. Louis, MO, USA) and dissolved in 0.5% v/v (final concentration) DMSO. Collagen and thrombin (≥ 10 NIH units/vial) were from Chrono-log Corporation (Havertown, PA, USA). Collagen-related peptide (CRP) was prepared as previously described (18) . FITC-conjugated mouse anti-human CD41a was from BD Biosciences (San Jose, CA, USA). PE-conjugated anti-human/mouse CD62p (P-Selectin) and anti-human Glycoprotein VI purified antibody were purchased from eBioscience (San Diego, CA, USA). FITC-conjugated anti-CD42b antibody was from Abcam (Cambridge, MA, USA). FITC-conjugated goat anti-mouse IgG was purchased from ZSGB-BIO (Beijing, China). -actin antibody and anti-rabbit IgG (HRP-linked) antibody were purchased from Cell Signaling Technology (Danvers, MA, USA). Tirofiban was purchased from Grand Pharma (China) CO. LTD.
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