Mice were anesthetized with 2,2,2-Tribromoethanol (350 mg/kg, Sigma-Aldrich, T48402) intraperitoneal injection, then placed into a stereotaxic frame. A small Hamilton syringe (33-gauge) was used to slowly inject 1 μl of saline or IL-10 (20 ng, Peprotech) or fluorocitrate (FC, 1 nmol, Sigma-Aldrich) per mouse into intracerebroventricular (i.c.v). After injection, the syringe was held in place for 5 min to avoid back-flow of cerebrospinal fluid (CSF). The stereotaxic coordinates were 1.34 mm posterior, 0 mm lateral to the bregma, and 2.3 mm ventral to the bregma.
Fluorocitrate fc
Manufactured by Merck Group
Sourced in United States
Fluorocitrate (FC) is a synthetic compound used as a laboratory tool for scientific research. It functions as an inhibitor, specifically targeting enzymes involved in the citric acid cycle. FC is employed in various experimental settings to study metabolic processes and pathways within biological systems.
Automatically generated - may contain errors
Lab products found in correlation
2 chloroethanol 2 ce, by Sinopharm (2 mentions)
Qpcr assay kits, by Takara Bio (2 mentions)
Gibh 130 gi, by Merck Group (2 mentions)
N acetyl l cysteine, by Selleck Chemicals (2 mentions)
Short hairpin rna shrna, by Hanbio Biotechnology (2 mentions)
Small interfering rnas sirnas, by RiboBio (2 mentions)
Sb202190, by Selleck Chemicals (2 mentions)
T48402, by Merck Group (1 mentions)
3 protocols using fluorocitrate fc
1
Intracerebroventricular Injection in Mice
2
Signaling Pathways Modulating Inflammatory Response
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2-Chloroethanol (2-CE) was obtained from Sinopharm Chemical Reagent Co., Ltd. (Ningbo, China), while reagents for the cell culture were purchased from Biological Industries (Beit-Haemek, Israel). The quantitative polymerase chain reaction (qPCR) assay kits were acquired from Takara (Japan). SB202190, pyrrolidine dithiocarbamate (PDTC), SR11302, and N-acetyl-l-cysteine (NAC) were purchased from Selleck (Houston, USA), APExBIO (Houston, USA), or Beyotime Biotechnology (Shanghai, China). Fluorocitrate (FC) and GIBH-130 (GI) were bought from Sigma (New Jersey, USA) and MedChemExpress (New Jersey, USA), respectively. Short hairpin RNA (shRNA) targeting the interleukin-1β (IL-1β) gene was designed and synthesized by Hanbio (Shanghai, China), while small interfering RNAs (siRNAs) targeting the tumor necrosis factor-α (TNF-α), inducible nitric oxide synthase (iNOS), or CYP2E1 genes were designed and synthesized by RiboBio (Guangzhou, China).
3
Reagents and Chemicals for Cell Culture
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2.1. Reagents 2-Chloroethanol (2-CE) was obtained from Sinopharm Chemical Reagent Co., Ltd. (Ningbo, China), while reagents for the cell culture were purchased from Biological Industries (Beit-Haemek, Israel). The quantitative polymerase chain reaction (qPCR) assay kits were acquired from Takara (Japan). SB202190, pyrrolidine dithiocarbamate (PDTC), SR11302, and N-acetyl-l-cysteine (NAC) were purchased from Selleck (Houston, USA), APExBIO (Houston, USA), or Beyotime Biotechnology (Shanghai, China). Fluorocitrate (FC) and GIBH-130 (GI) were bought from Sigma (New Jersey, USA) and MedChemExpress (New Jersey, USA), respectively. Short hairpin RNA (shRNA) targeting the interleukin-1β (IL-1β) gene was designed and synthesized by Hanbio (Shanghai, China), while small interfering RNAs (siRNAs) targeting the tumor necrosis factor-α (TNF-α), inducible nitric oxide synthase (iNOS), or CYP2E1 genes were designed and synthesized by RiboBio (Guangzhou, China).
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