Elecsys brahms pct assay

Manufactured by Roche

Sourced in Japan

The Elecsys BRAHMS PCT assay is a quantitative in vitro diagnostic test used for the measurement of procalcitonin (PCT) in human serum and plasma. PCT is a biomarker that can be used to aid in the diagnosis of sepsis and to monitor the progression of the disease.

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Lab products found in correlation

Dimension vista 1500 system, by Siemens (1 mentions) Modular e170 instrument, by Roche (1 mentions) Cobas e801 module, by Roche (1 mentions) Hiscl presepsin assay, by Sysmex (1 mentions) Maldi biotyper, by Bruker (1 mentions) Cobas e411 analyzer, by Roche (1 mentions) Crp latex 2 x2 seiken, by Denka Seiken (1 mentions)

Close spelling variants of this product

Elecsys assay (43 citations) Elecsys BRAHMS PCT (8 citations) BRAHMS PCT (3 citations) BRAHMS PCT assay (2 citations)

6 protocols using elecsys brahms pct assay

Procalcitonin Monitoring in ICU Patients

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It is common practice in our ICU to measure PCT daily in critically ill patients. Procalcitonin levels were documented from the previous day of enrollment (t₋₁) and immediately before the initiation of ABs (t₀). Core temperature, C-reactive protein (CRP), and white blood cell count (WBC) were also recorded with every PCT measure. The flow chart of the data collection is summarised in Figure 1.
Serum PCT levels were measured with Cobas 6000 analyzer (Hitachi High-Technologies Corporation, Tokyo, Japan). Analyzer reagents (Elecsys® B·R·A·H·M·S PCT assay) were developed in collaboration with B·R·A·H·M·S corporation (Hennigsdorf, Germany) and Roche Diagnostics (Mannheim, Germany). Procalcitonin was determined by electrochemiluminescence immunoassay (ECLIA) serum on the automated Roche Elecsys and Cobas immunoassay analyzers.

Trásy D., Tánczos K., Németh M., Hankovszky P., Lovas A., Mikor A., Hajdú E., Osztroluczki A., Fazakas J, & Molnár Z. (2016). Delta Procalcitonin Is a Better Indicator of Infection Than Absolute Procalcitonin Values in Critically Ill Patients: A Prospective Observational Study. Journal of Immunology Research, 2016, 3530752.

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PCT Measurement Using Elecsys Assay

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PCT concentrations were measured by the Elecsys BRAHMS PCT assay (Roche Diagnostics, Tokyo, Japan) using EDTA plasma as a sample.

Ishikura H., Nishida T., Murai A., Nakamura Y., Irie Y., Tanaka J, & Umemura T. (2014). New diagnostic strategy for sepsis-induced disseminated intravascular coagulation: a prospective single-center observational study. Critical Care, 18(1), R19.

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Measuring CRP and PCT in HSCT Patients

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Results of daily CRP and PCT measurements were recorded, as well as administration of drugs that could potentially influence their values such as corticosteroids, cytarabine, antithymocyte globulin and immunosuppressive therapy in case of allogeneic HSCT. CRP was measured daily by nephelometry using the Dimension Vista® 1500 System (Siemens Healthcare, Munich, Germany). PCT was measured daily using the Elecsys® BRAHMS PCT Assay on the Modular E170 instrument (Roche Diagnostics, Rotkreuz, Switzerland). This automated test is performed in human serum using the ECLIA (ElectroChemiLuminiscence ImmunoAssay) technique with a detection limit of 0.02 μg/L and an upper limit of normal (ULN) of 0.5 μg/L.

Verlinden A., De Vroey V., Goossens H., Roelant E., Van De Velde A.L., Berneman Z.N., Schroyens W.A, & Gadisseur A.P. (2019). Comparison of the Power of Procalcitonin and C-Reactive Protein to Discriminate between Different Aetiologies of Fever in Prolonged Profound Neutropenia: A Single-Centre Prospective Observational Study. Mediterranean Journal of Hematology and Infectious Diseases, 11(1), e2019023.

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Measurement of PCT and Presepsin Levels

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Residual EDTA plasma samples were aliquoted to avoid repeated freezing and thawing and were stored at –70°C until use. Frozen samples were thawed at room temperature and gently mixed immediately before measuring the PCT and presepsin levels. PCT level was measured using the Elecsys BRAHMS PCT assay (Roche Diagnostics GmbH, Mannheim, Germany) on a cobas e801 module (Roche Diagnostics GmbH) based on a electrochemiluminescence immunoassay. The manufacturer-suggested reference range for PCT was up to 0.046 ng/mL. Presepsin level was measured using the HISCL Presepsin assay (Sysmex, Kobe, Japan) in an HISCL 5000 automated analyzer (Sysmex) based on a delayed one-step sandwich chemiluminescence enzyme immunoassay. The manufacturer-suggested reference range for presepsin was up to 333.0 pg/mL. Both assays were performed according to the manufacturers’ instructions.

Park M., Hur M., Kim H., Lee C.H., Lee J.H., Kim H.W, & Nam M. (2022). Prognostic Utility of Procalcitonin, Presepsin, and the VACO Index for Predicting 30-day Mortality in Hospitalized COVID-19 Patients. Annals of Laboratory Medicine, 42(4), 406-414.

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Biomarker Measurement and Blood Culture Protocol

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CRP was measured by BioMajesty JCA-BM6070 (LEOL Ltd., Tokyo, Japan) using CRP-latex (II)X2 “Seiken” (Denka Seiken Co., Tokyo, Japan). Procalcitonin levels were measured by conducting an Elecsys BRAHMS PCT assay (Roche Diagnostics, Tokyo, Japan) using a Cobas e 411 analyzer (Roche Diagnostics). Presepsin levels were measured with a STACIA CLEIA Presepsin assay (LSI Medience Co., Tokyo, Japan) using the STACIA (LSI Medience Co.) system. Blood culture bottles were incubated in a BACTEC FX system (Becton, Dickinson and Co. Japan, Tokyo, Japan) for up to 7 days. Bacterial isolates recovered from blood cultures were identified by matrix-assisted laser desorption/ionization–time-of-flight mass spectrometry (MALDI–TOF MS) using a MALDI Biotyper (Bruker Daltonics, Kanagawa, Japan).

Matono T., Yoshida M., Koga H, & Akinaga R. (2022). Diagnostic accuracy of quick SOFA score and inflammatory biomarkers for predicting community-onset bacteremia. Scientific Reports, 12, 11121.

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Sepsis Biomarkers: PCT and PSEP

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Blood samples on day 0, in which the sepsis biomarkers could be measured, were routinely collected on admission to the ICU. PCT levels were measured using the Elecsys BRAHMS PCT assay (Roche Diagnostics, Tokyo, Japan). PSEP levels were measured using a compact automated immunoanalyser, PATHFAST ® , based on a chemiluminescent enzyme immunoassay (Mitsubishi Chemical Medicine, Tokyo, Japan).

Hoshino K., Irie Y., Mizunuma M., Kawano K., Kitamura T, & Ishikura H. (2017). Incidence of elevated procalcitonin and presepsin levels after severe trauma: a pilot cohort study. Anaesthesia and intensive care, 45(5).

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