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Hla class 1

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HLA Class I is a lab equipment product that functions as a major histocompatibility complex (MHC) class I molecule. It plays a central role in the immune system by presenting peptide fragments to T cells.

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Lab products found in correlation

Icam 1, by Cell Signaling Technology (2 mentions) Birc2, by Abcam (2 mentions) Birc3, by Cell Signaling Technology (2 mentions) Anti rabbit igg hrp conjugate, by Promega (2 mentions) β actin, by Merck Group (2 mentions) Chemidoc mp, by Bio-Rad (2 mentions)

2 protocols using hla class 1

1

Immunoblotting Protein Detection Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunoblotting was performed as described previously (Jacobs et al., 2008 (link)). Primary antibodies were followed by mouse- or rabbit-conjugated horseradish peroxidase (HRP) secondary antibodies. HRP-conjugated antibodies (anti-mouse or anti-rabbit IgG HRP conjugate, Promega) were detected by enhanced chemiluminescence detections (Thermofisher) using a Biorad ChemiDoc MP. This included the following antibodies: BIRC2 (108361, Abcam), BIRC3 (3130, Cell Signaling), HLA Class I (70328, Abcam), ICAM1 (4915, Cell Signaling), IRF1 (8478 Cell Signaling), XIAP (2042, Cell Signaling). Alternatively, primary antibodies were followed by fluorescently labeled anti-mouse or rabbit antibodies and imaged using a Biorad ChemiDoc MP. This included the following antibodies: β-actin (A5441, Sigma).
Kishton R.J., Patel S.J., Decker A.E., Vodnala S.K., Cam M., Yamamoto T.N., Patel Y., Sukumar M., Yu Z., Ji M., Henning A.N., Gurusamy D., Palmer D.C., Stefanescu R.A., Girvin A.T., Lo W., Pasetto A., Malekzadeh P., Deniger D.C., Wood K.C., Sanjana N.E, & Restifo N.P. (2022). Cancer genes disfavoring T cell immunity identified via integrated systems approach. Cell reports, 40(5), 111153.
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2

Immunoblotting Protein Detection Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunoblotting was performed as described previously (Jacobs et al., 2008 (link)). Primary antibodies were followed by mouse- or rabbit-conjugated horseradish peroxidase (HRP) secondary antibodies. HRP-conjugated antibodies (anti-mouse or anti-rabbit IgG HRP conjugate, Promega) were detected by enhanced chemiluminescence detections (Thermofisher) using a Biorad ChemiDoc MP. This included the following antibodies: BIRC2 (108361, Abcam), BIRC3 (3130, Cell Signaling), HLA Class I (70328, Abcam), ICAM1 (4915, Cell Signaling), IRF1 (8478 Cell Signaling), XIAP (2042, Cell Signaling). Alternatively, primary antibodies were followed by fluorescently labeled anti-mouse or rabbit antibodies and imaged using a Biorad ChemiDoc MP. This included the following antibodies: β-actin (A5441, Sigma).
Kishton R.J., Patel S.J., Decker A.E., Vodnala S.K., Cam M., Yamamoto T.N., Patel Y., Sukumar M., Yu Z., Ji M., Henning A.N., Gurusamy D., Palmer D.C., Stefanescu R.A., Girvin A.T., Lo W., Pasetto A., Malekzadeh P., Deniger D.C., Wood K.C., Sanjana N.E, & Restifo N.P. (2022). Cancer genes disfavoring T cell immunity identified via integrated systems approach. Cell reports, 40(5), 111153.
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