Thiobarbituric acid tba 1 1 3 3 tetraethoxypropane tep

The black tea (Lapsang souchong) was purchased from Wuyi Mountain Zhengshan Tea Industry Co., Ltd, China. The 2,2ʹ-Biquinoline-4,4ʹ-dicarboxylic acid (BCA), sodium tartrate dibasic dihydrate, sodium carbonate, sodium hydroxide, sodium bicarbonate, hydrochloric acid, copper (II) sulphate pentahydrate, disodium hydrogen phosphate dodecahydrate, Sodium dihydrogen phosphate dihydrate, 4-Aminoantipyrine, potassium ferricyanide trihydrate, sodium 3,5-dichloro-2-hydroxybenzenesulfonate, Triton X-100, Lithium carbonate, Sodium acetate, acetic acid, iron (III) chloride hexahydrate, 2,4,6-Tris(2-pyridyl)-s-triazine (TPTZ), iron (II) sulphate heptahydrate and trichloroacetic acid (TCA) were obtained from Sinopharm Chemical Reagent Co., Ltd, Shanghai, China. The bovine serum protein (BSA), peroxidase from horseradish, ascorbate oxidase from Cucurbita sp., urease from Candida sp., uric acid, 5,5ʹ- dithiobis-(2-nitrobenzoic acid) (DTNB), Tris(hydroxymethyl)aminomethane (Tris), L-cysteine, thiobarbituric acid (TBA), 1,1,3,3-Tetraethoxypropane (TEP) were purchased from Sigma-Aldrich Co. Ltd, Shanghai, China.

All the solutions employed were prepared with deionized water (Milli-Q Merck, Millipore Darmstadt, Hesse, Germany) with a resistivity of 18.2 MΩ cm or greater. All chemicals used were analytical grade and used without further purification. Reagents employed for the pumpkin flower foam-mat elaboration; maltodextrin, hydroxyethyl cellulose, and egg albumin (EA) were purchased from Food Technologies Trading (Estado de Mexico, Mexico), Tween-80 was purchased from J.T. Baker (Phillipsburg, NJ, USA).
Reagents employed for the antioxidant activity assays; 2,2-Diphenyl-1-picrylhydrazyl (DPPH), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), potassium persulfate, 2,4,6-tris-2-piridil-s-triazine (TPTZ), hydrochloric acid, acetic acid, Folin–Ciocalteu reagent, gallic acid, thiobarbituric acid (TBA), 1,1,3,3-tetraethoxypropane (TEP), and methanol (MeOH) were purchased from Sigma Aldrich (St. Louis, MO, USA). ABTS was purchased from Roche Diagnostics (Indianapolis, IN, USA). Trichloroacetic acid (TCA) was purchased from Meyer (Estado de Mexico, Mexico). Iron (II) sulfate, sodium carbonate, and sodium acetate were obtained from J.T. Baker (Phillipsburg, NJ, USA). Ferric (III) chloride was purchased from Merck (Darmstadt, Hesse, Germany).