Assaymax corticosterone elisa kit

Manufactured by Assaypro

Sourced in United States

The AssayMax Corticosterone ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of corticosterone levels in biological samples. The kit utilizes a specific antibody coated on a 96-well plate and a detection antibody conjugated to horseradish peroxidase to enable the colorimetric detection and quantification of corticosterone.

Automatically generated - may contain errors

Lab products found in correlation

Multiskan fc, by Thermo Fisher Scientific (2 mentions) Mouse ige elisa kit, by R&D Systems (1 mentions) Nembutal, by Ceva (1 mentions) Fluostar omega, by BMG Labtech (1 mentions) Nefa c test wako, by Fujifilm (1 mentions) Glucose cii test wako, by Fujifilm (1 mentions) Mouse rat igf 1 quantikine elisa kit, by R&D Systems (1 mentions)

20 protocols using assaymax corticosterone elisa kit

Serum Corticosterone ELISA Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Serum corticosterone was assayed using the AssayMax Corticosterone ELISA kit (Assaypro, Catalog no. EC3001-1; http://www.assaypro.com/), according to the instructions of the manufacturer. This kit employs a quantitative competitive enzyme immunoassay technique. A standard and/or serum sample (25 μL) was added to each well of a 96-well microplate precoated with a corticosterone-specific polyclonal antibody, followed by the addition of 25 μL of biotinylated corticosterone. After 2 h of incubation, the wells were washed five times with the wash buffer. Streptavidin-peroxidase (50 μL) was then added to each well, and the mixtures were incubated for 30 min. After washing five times with wash buffer, 50 μL of the chromogen substrate was added per well. The reaction mixtures were incubated until the optimal blue color density was observed. After adding 50 μL of the stop solution, the absorbance was read immediately on a microplate reader at a wavelength of 450 nm. Finally, the mean value of the triplicate readings for each standard and serum sample was calculated, and the unknown sample concentration was determined from the standard curve.

Chen L., Dai J., Wang Z., Zhang H., Huang Y, & Zhao Y. (2014). Ginseng Total Saponins Reverse Corticosterone-Induced Changes in Depression-Like Behavior and Hippocampal Plasticity-Related Proteins by Interfering with GSK-3β-CREB Signaling Pathway. Evidence-based Complementary and Alternative Medicine : eCAM, 2014, 506735.

+ Open protocol

+ Expand

Urinary Corticosterone Measurement in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Urine samples from mice were collected in the morning on day 7 of the 28-day restraint regimen. Concentrations of urinary corticosterone in the samples were determined using the AssayMax Corticosterone ELISA kit (Assaypro, St. Charles, MO) according to the manufactures protocol.

Katsube T., Wang B., Tanaka K., Ninomiya Y., Hirakawa H., Liu C., Maruyama K., Vares G., Liu Q., Kito S., Nakajima T., Fujimori A, & Nenoi M. (2021). Synergistic Effects of Chronic Restraint-Induced Stress and Low-Dose 56Fe-particle Irradiation on Induction of Chromosomal Aberrations in Trp53-Heterozygous Mice. Radiation research, 196(1).

+ Open protocol

+ Expand

Serum Corticosterone Measurement in Mice

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mice were transported from the animal colony to the laboratory space and allowed to acclimate for 1 h. Trunk blood was collected by rapid decapitation. To eliminate potential cohort effects, a minimum of at least 2 different litters were used for each developmental time point. Serum corticosterone levels were measured using a competition-based ELISA (AssayMax, Corticosterone ELISA Kit, AssayPro, St. Charles, MO) using the manufacturers protocol. This kit reports a sensitivity of up to 0.3 ng/mL, with a 5–7% intra-assay reliability, and <2% cross reactivity with steroid and stress-related hormones. With this assay, we have observed an intra-assay reliability of 5.5%.

Bath K.G., Manzano-Nieves G, & Goodwill H. (2016). Early life stress accelerates behavioral and neural maturation of the hippocampus in male mice. Hormones and behavior, 82, 64-71.

+ Open protocol

+ Expand

Plasma Corticosterone Levels in Rats

Check if the same lab product or an alternative is used in the 5 most similar protocols

Plasma corticosterone (CORT) was determined before and after predator odor exposure. Three days before treatment, blood from the saphenous vein was drawn to measure basal levels of CORT and measured again on control and stressed rats on the day of euthanasia (Day 6). After pentobarbital injection, blood was drawn from cardiac puncture, collected in 1 mg/mL EDTA- containing microtubes, centrifuged at 3000×g for 10 min, then plasma was separated, and stored at −80 °C. CORT concentration was measured using the AssayMax Corticosterone ELISA kit (Assaypro EC3001-1) following manufacturer’s instructions. Results are expressed as ng of CORT per mL of plasma.

Mejia-Carmona G.E., Gosselink K.L., Pérez-Ishiwara G, & Martínez-Martínez A. (2015). Oxidant/antioxidant effects of chronic exposure to predator odor in prefrontal cortex, amygdala, and hypothalamus. Molecular and Cellular Biochemistry, 406(1-2), 121-129.

+ Open protocol

+ Expand

Serum IgE and Corticosterone Measurement

Check if the same lab product or an alternative is used in the 5 most similar protocols

Serum levels of total IgE and corticosterone were determined by using the mouse IgE ELISA kit (R&D, Minneapolis, MN, USA, or Morinaga Institute of Biological Science, Kanagawa, Japan) or AssayMax Corticosterone ELISA kit (AssayPro, Charles, MO, USA), respectively.

Nakamura Y., Ishimaru K., Tahara Y., Shibata S, & Nakao A. (2014). Disruption of the Suprachiasmatic Nucleus Blunts a Time of Day-Dependent Variation in Systemic Anaphylactic Reaction in Mice. Journal of Immunology Research, 2014, 474217.

+ Open protocol

+ Expand

Urine Corticosterone Measurement Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Urine samples were collected after the AD induction phase in the 3^rd week and the stress loading phase during the 1^st and 2^nd weeks. Urine samples were collected 2 h after the last stress loading, and were then stored in 2-ml tubes at −80 °C until analysed. A volume of 1.5 μl was used and diluted at a ratio of 1:20 into a diluent for the analysis. Urine corticosterone levels were measured using the AssayMax™ Corticosterone ELISA kit (Assaypro, MO, USA) according to the manufacturer’s instructions.

ZHAO Q., TOMINAGA M., TOYAMA S., KOMIYA E., TOBITA T., MORITA M., ZUO Y., HONDA K., KAMATA Y, & TAKAMORI K. (2024). Effects of Psychological Stress on Spontaneous Itch and Mechanical Alloknesis of Atopic Dermatitis. Acta Dermato-Venereologica, 104, 18685.

+ Open protocol

+ Expand

Corticosterone ELISA Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Serum corticosterone levels were quantified using the AssayMax Corticosterone ELISA Kit (Assay Pro, MO, USA) according to the manufacturer’s protocol.

Du Y., Kusama K., Hama K., Chen X., Tahara Y., Kajiwara S., Shibata S, & Orihara K. (2024). Protective Effects of Inulin on Stress-Recurrent Inflammatory Bowel Disease. International Journal of Molecular Sciences, 25(5), 2494.

+ Open protocol

+ Expand

Serum Corticosterone Measurement

Check if the same lab product or an alternative is used in the 5 most similar protocols

To measure serum corticosterone levels, we anesthetized the animals and collected blood samples by removing an eyeball at two time points (post-AT and post-2W). Blood samples were always collected at a specific time (4–6 PM). Blood samples were allowed to stand for 60 min at room temperature, and then centrifuged at 5000 rpm for 20 min at 4°C. Serum was then collected and stored at −80°C to await corticosterone assay. The serum corticosterone level was determined by an AssayMax corticosterone ELISA kit (AssayPro, St. Charles, United States).

Li P., Bing D., Wang S., Chen J., Du Z., Sun Y., Qi F., Zhang Y, & Chu H. (2019). Sleep Deprivation Modifies Noise-Induced Cochlear Injury Related to the Stress Hormone and Autophagy in Female Mice. Frontiers in Neuroscience, 13, 1297.

+ Open protocol

+ Expand

Corticosterone Levels After Fear Training

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mice were randomly divided into five groups: no training, training 90 min, training + stress (60–90 min), training + stress (90–120 min), and immobilization stress only. Ninety minutes after exposure the training chamber without footshock, the no training mice were sacrificed to collect the blood samples. Training 90 min mice were sacrificed to collect the blood 90 min after the training. Training + stress (60–90 min) and training + stress (90–120 min) mice were sacrificed to collect the blood immediately after the immobilization stress following training. Immobilization stress only mice were sacrificed to collect the blood sample immediately after the immobilization stress. The blood samples were used for corticosterone measurement (Fig. 4a).Fig. 4

Plasma corticosterone levels (Experiment 5). a Experimental protocol for blood sample collection. b Comparison of plasma corticosterone levels (n = 5). Data are expressed as mean ± SEM. *P < 0.05 compared with no training group. ^#P < 0.05 compared with training group. ^@P < 0.05 compared with training + stress group (90–120 min)

Blood samples were collected after contextual fear training and immobilization stress. Plasma corticosterone levels were quantified by ELISA according to the instructions of the supplier (AssayMax Corticosterone ELISA Kit, AssayPro LLC, USA).

Uwaya A., Lee H., Park J., Lee H., Muto J., Nakajima S., Ohta S, & Mikami T. (2016). Acute immobilization stress following contextual fear conditioning reduces fear memory: timing is essential. Behavioral and Brain Functions : BBF, 12, 8.

+ Open protocol

+ Expand

Quantitative Corticosterone ELISA Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Plasma corticosterone was assayed using the AssayMax Corticosterone ELISA kit that employs a quantitative competitive enzyme immunoassay technique (Assaypro, Catalog No. EC3001–1) following the manufacturer’s instructions. The unknown sample concentration was determined from the standard curve. The mean value of the triplicate readings for each sample was calculated.

Zhao Y., Zhang Q., Shao X., Ouyang L., Wang X., Zhu K, & Chen L. (2017). Decreased Glycogen Content Might Contribute to Chronic Stress-Induced Atrophy of Hippocampal Astrocyte volume and Depression-like Behavior in Rats. Scientific Reports, 7, 43192.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!